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Offlineshadowpuppets
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Last Chance with Spores. Need Help!
    #26819583 - 07/12/20 02:33 PM (3 years, 7 months ago)

Here is the thing:



I have failed many agar transfers and lost many grain jars. I never got a clean culture on any agar plate and never got a clean spawn. It could be the main source of culture, I don’t know. But now, I am going to go for plan B, which is the spore vial.

I have 1 cubensis spore vial and many syringes. My plan is to stretch spores and make many syringes, because this spore vial is my last chance to grow mushrooms. I don’t have access to spores where I live.



Here is my plan:

  • I will aspirate half of spores in a syringe (just because I might fail). I will inject some spores to a jar with sterilized water, and fill other syringes through the SHIP.
  • I will inoculate couple grain jars with the original syringe.
  • I will inoculate some agar dishes with couple drops (I didn’t say I am giving up on agar!)

As you can tell, I am trying to make the most out of the spore vial! Please let me know if you have any additional beneficial idea.


Here are my questions:



  • As you see, my syringe is incomplete and I have to assemble it. I think I have to be very careful, because the air might go inside the syringe and fuck things up. I will do it in SAB, but still, I will sterilize this empty syringe before aspirating any spores. But how do I sterilize empty syringe? Will PC cycle do the job? I read couple teks, but still not sure about sterilizing an empty syringe.
  • Since the plan is to stretch spores, I need sterilized water in a jar. How do I sterilize a jar with water in it? I mean, how long should it take to sterilize water in PC?
  • Should I use tap water or distilled water?


And here comes the most crucial question: What would you do if you were me, considering that this spores are your only chance for your growing career? I really would love to hear your opinion, especially from TCs. I am going to post to make this thread active, because I really do want to grow shrooms, as I like them, I invested for them, and I have done many research and I don't want them to be such a waste. Should I have more questions, I will post them. So please help!

Am I overthinking?


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OfflineTrichodingus
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Re: Last Chance with Spores. Need Help! [Re: shadowpuppets] * 1
    #26819612 - 07/12/20 02:41 PM (3 years, 7 months ago)

Would you walk me through your SAB procedure regarding agar transfers?

Once you have myc on a plate you should be able to get clean cultures even if it was contaminated at some point.

I'd personally ditch the idea of trying to inoculate jars. It's basically roulette.


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Offlineshadowpuppets
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Re: Last Chance with Spores. Need Help! [Re: Trichodingus]
    #26819886 - 07/12/20 05:15 PM (3 years, 7 months ago)

Inoculating the jars wouldn't be a waste for me. I'm ready to lose couple grains. I'll give it a try anyway.

I can walk you through my SAB procedure, but it's the same as Spitball's, or Bod's. Spraying SAB with ISO and soapy water, towel, gloves, mask etc. I was even rubbing every single agar plate with ISO soaked paper, which no one does.

Believe me, my sterile procedure is very tight. I failed because I made many many tranfers that I believe that my current culture is not doing well because of senescence. So I'm going for plan B. So I need help for plan B.


Edited by shadowpuppets (07/12/20 05:19 PM)


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OfflineTrichodingus
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Re: Last Chance with Spores. Need Help! [Re: shadowpuppets]
    #26819908 - 07/12/20 05:33 PM (3 years, 7 months ago)

Do what you want, but if that's all the spores you have access to, spores to grains are the riskiest way to go except maybe spores -> LC -> grains.

If you only have spores and want to skip agar for now, PF Tek is the way to go.

At least you'd be able to get some prints, a few fruits and be able to try grains with spores in reserve.


Edited by Trichodingus (07/12/20 05:51 PM)


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OfflineA.k.aM
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Re: Last Chance with Spores. Need Help! [Re: Trichodingus]
    #26819940 - 07/12/20 05:53 PM (3 years, 7 months ago)

Have you tried a hot pour?

Agar is made to clean up cultures there’s no reason you shouldn’t be able to do it.

If the contam is meshed in a hot pour could solve the problem.

Just pour a layer of agar on top of the dirty plate, like the one in the picture, and wait for the myc to grow to the surface. Then immediately transfer it.

Also syringes come sterile, you won’t need to do anything.


--------------------
LAGM2020


Edited by A.k.a (07/12/20 06:13 PM)


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Offlinemetaphoric
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Re: Last Chance with Spores. Need Help! [Re: Trichodingus] * 2
    #26819944 - 07/12/20 05:55 PM (3 years, 7 months ago)

My 2 cents:

You are 1000% not experiencing senescence.

If you are going to do spores to grain,  wait until it fully colonized, then open it in a SAB and transfer a few grains to a few agar plates before you spawn the rest to your bulk sub.
This is simply a backup in case the grains go south and you're out of spores. A grain on agar is a great way to begin cleaning up your culture.


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineLogicaL ChaosM
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Re: Last Chance with Spores. Need Help! [Re: shadowpuppets]
    #26820012 - 07/12/20 06:53 PM (3 years, 7 months ago)

Try add a drop of Lysol or soap to your spore syringe then shake well (use a petri dish) It should fix your issue.


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Offlineshadowpuppets
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Re: Last Chance with Spores. Need Help! [Re: metaphoric]
    #26821106 - 07/13/20 01:16 PM (3 years, 7 months ago)

Quote:

Trichodingus said:
Do what you want, but if that's all the spores you have access to, spores to grains are the riskiest way to go except maybe spores -> LC -> grains.

If you only have spores and want to skip agar for now, PF Tek is the way to go.

At least you'd be able to get some prints, a few fruits and be able to try grains with spores in reserve.




Man, inoculating grains is only one of my plans. I will inoculate with only some drops, not even 1 cc. And I've never said I am skipping agar, actually I said I will inoculate agar dishes as well. Please re-read my post. My main goal is to stretch spores with sterilized water. But well said on PF Tek! I can prepare some PF jars and inoculated them with my other (stretched) syringes.

Quote:

A.k.a said:
Have you tried a hot pour?

Agar is made to clean up cultures there’s no reason you shouldn’t be able to do it.

If the contam is meshed in a hot pour could solve the problem.

Just pour a layer of agar on top of the dirty plate, like the one in the picture, and wait for the myc to grow to the surface. Then immediately transfer it.

Also syringes come sterile, you won’t need to do anything.




What do you mean by hot pour? I pour my agar plates and transfer, but almost all of them went to thrash. But I've never poured agar on top of a dirty plate, what is this tek?

I really don't know what I am doing wrong, but I always fail while doing agar transfers. Here are my best looking agar plates currently and I don't think I'll get a clean culture from those plates:



Yes syringes are sterile, but mine is not assembled, so it will be contaminated when I try to assemble it. So that's why I need to sterilize them. And I dont know how to sterilize empty syringes. You have any ideas?


Quote:

metaphoric said:
My 2 cents:

You are 1000% not experiencing senescence.

If you are going to do spores to grain,  wait until it fully colonized, then open it in a SAB and transfer a few grains to a few agar plates before you spawn the rest to your bulk sub.
This is simply a backup in case the grains go south and you're out of spores. A grain on agar is a great way to begin cleaning up your culture.




Yes, this whole culture is actually from grain to agar! I had a growkit where I inoculated all 5 cc spores to grain (I wasn unaware of shroomery), and after reading a lot, I came up with the idea of grain to agar which is not very common. But as I said, I am not going to use all my spores inoculating grain jars.

Summary:

My main goal is to stretch spores like bodhisatta said (see below), and make the most out of my spore vial. That being said, I need to a jar with sterilize water (tap or distilled?) and sterilize empty stringes. But how do I sterilize them? After I have 5-10 syringes, I will try every, literally every method!


https://www.shroomery.org/forums/showflat.php/Number/19132583

Quote:

bodhisatta said:
Quote:

FrankHorrigan said:
Quote:

bodhisatta said:
1 syringe has enough spores to inoculate 1000s of jars




Would love to see you try inoculating 1000 1/2 pints with a 10mL syringe :rofl:



Easy. Take one syringe and inject it into 300-500mL of water. Then aspirate it back into syringes. Youll have 30-50 of em enough to do 1000 half pints no problem. The syringe has enough spores. Didn't say i could do it with just the one syringe though. Have to do some diluting first.

One syringe has enough to do 100,000 or more. But i think you would run into the problem of some drops not having spores at all at that dilution




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InvisibleRoger Clemency
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Re: Last Chance with Spores. Need Help! [Re: shadowpuppets]
    #26821115 - 07/13/20 01:22 PM (3 years, 7 months ago)

Those plates look okay to me, most of them. Not fully clean any of them but there is myc to be transferred.

I just spray my SAB with water and work deliberately, careful not to bump the walls. You may be doing something wrong in there if you keep getting bad stuff along with your transfers.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends


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Offlinemetaphoric
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Re: Last Chance with Spores. Need Help! [Re: Roger Clemency]
    #26821134 - 07/13/20 01:35 PM (3 years, 7 months ago)

I don't understand why you need to stretch your spores if you're working with agar.


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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Offlineshadowpuppets
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Re: Last Chance with Spores. Need Help! [Re: metaphoric]
    #26825503 - 07/15/20 03:57 PM (3 years, 6 months ago)

Quote:

Roger Clemency said:
Those plates look okay to me, most of them. Not fully clean any of them but there is myc to be transferred.

I just spray my SAB with water and work deliberately, careful not to bump the walls. You may be doing something wrong in there if you keep getting bad stuff along with your transfers.




The mycelium is not vigorous. That's why I don't think next transfers will be fine to inoculate grains.

Quote:

metaphoric said:
I don't understand why you need to stretch your spores if you're working with agar.




It is because I keep failing agar transfers. I will try every possible way to grow mushrooms, so I need more (stretched) spore solution.


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