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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Green with frustration (updated with deeper mystery!)
    #26814932 - 07/10/20 11:33 AM (3 years, 7 months ago)

So I've recently had to dump 4 tubs before they even pinned due to trich.  Before that I got trich in 2 tubs the day before I harvested the first flush.  Yesterday I did harvest a tub of Avery's Albino, and so far it's still okay, we'll see...  I'm just getting so damn frustrated over this.  I know it's supposed to come from the spawn but I have seen some people blame the coir, oats, and the growing area.

I start on malt extract agar, transfer until clean, and then make a few plates of each strain.  I then do A2G, all of this so far happens inside the SAB.  My lids are just the normal 2 part mason jar lids, 1/4 inch hole drilled, full SFD on top of the metal lid, under the ring.  I use oats as my grain, switched from WBS due to ease of prep.  I put a pinch of gypsum in the boil for each jar.  Boil oats for about 35-40 mins, drain for 2 hours probably.  Pressure cook 10 quart jars at 15-18psi for 1 hour 45, but since having all these problems I switched to 2 hours yesterday. 

I use the bucket tek exactly as written and sometimes I'll do a double batch in a cooler.  I have been adding some gypsum to the substrate for the last month or so, but I feel these problems have been worse since adding the gypsum, so I will no longer be doing that, however I do want to keep it in the oat jars because it helps with the separation of the grains. 

I spawn in open air inside my home, into bus tubs that fit inside of Bod's unmodified tubs.  I've been keeping the lids on, not upside down, but with the gasket's removed because in the past I had issues with the sub drying out.  Now that I think about it though, with the bus tubs in there now, it might not dry out as the bus tub has a little room all around it inside the unmodded tub.  The drying issue was always around the edges before... Hmm. 

I am willing to changed anything to get this to work properly.  I even ordered some pasteurized compost online just to see if that would go differently.  My jars always look and smell ok before I spawn, if they don't, I don't chance it.  The agar plates I use for A2G are always pretty too.  My agar plates rarely contam, unless it's completely hidden and this is my problem, so I don't see why my jars that are A2G'd in the same SAB would give me so much bad spawn.  Also, would the fact that so many tubs went green in the same area be a problem?  I tried to clean as best as I could, I sprayed a shit ton of alcohol on the whole area and I clean out the tubs with bleach and alcohol too.  But I do put the new tubs back into this same area.....


Someone help me please.


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"It says number one son on the license plate. That's me. I'm the number one son."
"What about me, Mom?"
"You're number one son too"


Edited by X-Ray Cat (08/12/20 10:10 AM)


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,750
Re: Green with frustration [Re: X-Ray Cat] * 1
    #26814948 - 07/10/20 11:37 AM (3 years, 7 months ago)

Got plate pics?


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: LadysKnight] * 1
    #26815002 - 07/10/20 11:57 AM (3 years, 7 months ago)

Hey LK, thanks for reading all of that.  I was going to A2G these today.



--------------------

"It says number one son on the license plate. That's me. I'm the number one son."
"What about me, Mom?"
"You're number one son too"


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Re: Green with frustration [Re: X-Ray Cat]
    #26815006 - 07/10/20 12:00 PM (3 years, 7 months ago)

Did you have the same issues with WBS?


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My Magnum Opus:Thai Variety Comparison

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OfflineSFS96
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Registered: 12/09/18
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Re: Green with frustration [Re: ManifoldPrime] * 1
    #26815022 - 07/10/20 12:09 PM (3 years, 7 months ago)

Those plates look good. It could be your A2G technique or maybe something is up with your grains. I personally don’t like using SFDs with 2 piece lids because u have to grab the filter and the jar lid to open for A2G. I prefer using a SFD under a plastic lid or a piece of SFD silicone too the top of a plastic lid. I really like the ball leak proof lids, they hold up to pressure cooking really  well.


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: ManifoldPrime] * 1
    #26815032 - 07/10/20 12:13 PM (3 years, 7 months ago)

With WBS I would get usually 2 flushes before trich.  But the 2 tubs that contamed right before first flush harvest were WBS.  And lots of people love oats so I don't see why they could be that much worse.

Here are some jars that are just about done, just need to consolidate for a few days.



The camera made them look sort of weird, they're whiter in actuality.

Edit, this pic is a bit more realistic.


I will check into those lids SFD, sounds better than throwing away rusty ones all of the time.  Although I don't find them too troublesome in the SAB, I just use the thread of the ring to pop the seal on the metal lid and kind of lean the whole assembly to the side while I drop the agar in real quick, then back down quickly with the lid assembly.


Edited by X-Ray Cat (07/10/20 12:26 PM)


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InvisibleLadysKnight
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Registered: 10/09/15
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Re: Green with frustration [Re: X-Ray Cat] * 1
    #26815247 - 07/10/20 01:46 PM (3 years, 7 months ago)

Plate pics look good, but not enough surface light to be sure.
Jars look a little off. How does everything smell?


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OfflineElViajero
Traveler


Registered: 08/16/18
Posts: 295
Last seen: 2 years, 10 months
Re: Green with frustration [Re: LadysKnight]
    #26815249 - 07/10/20 01:47 PM (3 years, 7 months ago)

yeah, jars look off. maybe take a pic of how jars look like before inoculating.


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: ElViajero] * 1
    #26815330 - 07/10/20 02:24 PM (3 years, 7 months ago)

Hmm I shook those jars maybe 4 or five days ago so all of those little points of mycelium are growing together into one.  The pics make them look very weird, there's not that much contrast in real life, brighter white too.  I took a close up of one here...

If that still looks weird could you guys kindly point out the areas that look bad?

Here are some jars that aren't as far along.


Here's an uninnoculated jar that was made yesterday.


Thank you guys so much for helping me get to the bottom of this.  Oh, and as far as smells go everything just smells mushroomy.


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Re: Green with frustration [Re: X-Ray Cat]
    #26815357 - 07/10/20 02:39 PM (3 years, 7 months ago)

I cant quite tell if theres anything actually wrong with those jars, I'm new to oats myself. the Pic with 2 jars, on the bottom left, theres a spot that looks a bit hazy, but that could just be lighting. I'd be happy using those jars if they were mine however. I would just make sure they survived a final shake at 100%

Onto the next thing, your coco coir could also be at fault. some Coco is actually treated with mold spores, especially cannabis growing coir from hydroponics stores, from what I've read here.
Maybe sterilise your coir for some of your next grows(Mushboy's shoebox thread OP has details on sterilising coir. I've done it many times. Just be sanitary when spawning)
Also, clean your misting/spraying bottle, tubs, and everything else you use when spawning thoroughly.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


Edited by ManifoldPrime (07/10/20 02:40 PM)


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InvisibleLadysKnight
Hello Ladies
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Registered: 10/09/15
Posts: 1,750
Re: Green with frustration [Re: ManifoldPrime] * 1
    #26815398 - 07/10/20 02:57 PM (3 years, 7 months ago)

Yeah, the new pics look a lot better, the others looked a little grey and thin. As MP said, definitely could be the coir. But I'd still like another look at the plates with better lighting.


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: LadysKnight]
    #26815401 - 07/10/20 02:59 PM (3 years, 7 months ago)

That hazy part is a reflection from the window behind me, and I'm using eco earth for the coir.


--------------------

"It says number one son on the license plate. That's me. I'm the number one son."
"What about me, Mom?"
"You're number one son too"


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OfflineSFS96
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Registered: 12/09/18
Posts: 2,144
Loc: Valleys Of Neptune
Last seen: 21 hours, 26 minutes
Re: Green with frustration [Re: X-Ray Cat]
    #26815885 - 07/10/20 07:29 PM (3 years, 7 months ago)

Those jars definitely look off. I can’t really explain why lol once you know how there supposed to look you can normally tell when there off. The mycelium just doesn’t look right, there’s a hidden contamination for sure.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: SFS96]
    #26815941 - 07/10/20 07:54 PM (3 years, 7 months ago)

Ugh I feel what you're saying SFS, I guess I've just been lying to myself because everything is white in the jar.  The close-ups do look kinda weird though.  Just over this crap, so much work into the trash over and over again.  I will definitely be getting those plastic lids for the jars too, just did some more A2G with some brand new metal lids and they were a BITCH.  It's a sign lol.  I just don't understand where the contam is coming from, my plates always look nice.  I'm starting new genetics from spores right now, also doing the oats for 2 hours from now on.  I might break down and build a flow hood too... Actually yes, that's happening, I hate working in the SAB anyways.


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: X-Ray Cat]
    #26819447 - 07/12/20 01:36 PM (3 years, 7 months ago)

So you guys were totally right about those jars.  I was going to spawn them just for shits and I shook them all up.  When I went to spawn maybe 40 mins later I smelled them and they all smelled a little sweet and fruity, but like a bad kinda fruity.  I did spawn 3 of the same jars before that though, into some pasteurized compost I ordered online.  It needed to be used and all I had was the suspect spawn... Whatever, I don't have high hopes.

Do you guys think I would be more successful with a flow cabinet/hood?  I'm probably going to build one here soon as I hate working in the SAB anyways.

All of these suspect jars came from some old plates of APE, they were overgrown and touching the sides of the petri dish, do you think that could have been an issue?  I'm going to only use fresh plates from now on.

Thanks again for the help y'all.


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Invisibleretowen
Forest Dweller
Registered: 08/05/16
Posts: 51
Loc: Canada
Re: Green with frustration [Re: X-Ray Cat]
    #26819470 - 07/12/20 01:50 PM (3 years, 7 months ago)

I feel your pain


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: retowen]
    #26819499 - 07/12/20 02:01 PM (3 years, 7 months ago)

I was pissed the other day but now I view this as an opportunity for growth.  I think it's funny though, back in the day I didn't know any better and would just shoot spores right into grain jars and I had so much success!  Now I know better and I'm still struggling.  All part of the path to being a bomb cultivator I suppose.


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OfflineX-Ray Cat
Sees through wooden doors

Registered: 05/12/20
Posts: 134
Last seen: 7 months, 10 days
Re: Green with frustration [Re: X-Ray Cat]
    #26876652 - 08/12/20 10:24 AM (3 years, 6 months ago)

OK y'all back again here with more mysteries, or maybe not actually.  SO, the jars that we all agreed were bad, see above, were thrown away minus 3.  Those 3 were spawned to some pasteurized compost mix from shrooms supply.  This thing colonized and sat idle for 2 weeks or so, no problems.  They are APE, which I have no experience with.  I thought a pseudo-casing would be good enough for them, it wasn't.  I spawned the other jars pictured above, the ones with the nice growth that look like they had been shaken a few days before the pic.  At this time I used some of that coir to case the APE because it was just sitting there in quarantine doing nothing.

Now get this!

These guys popped up and that tub is still problem free, exactly a month later.

Meanwhile.... Those jars that looked good got spawned to unpasteurized coir and got some nasty shit in there right around the time they started pinning.

So does this count as a mark against coir and for proper pasteurization?


--------------------

"It says number one son on the license plate. That's me. I'm the number one son."
"What about me, Mom?"
"You're number one son too"


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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Green with frustration [Re: X-Ray Cat]
    #26876692 - 08/12/20 10:53 AM (3 years, 6 months ago)

Trich is a bitch. We are also having issues determining the contam factor. Many people say it cant be the coir and it must be the grain spawn, but i am starting to suspect it is. Tagged


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Green with frustration [Re: maxmush]
    #26876699 - 08/12/20 11:00 AM (3 years, 6 months ago)

Yes those jars really looked off. They kind of had this matted look and grew in this weird way that they should not have. So the contam could have been the jars definitely.


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