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Offlinepitriot
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Making Liquid Cuture?
    #26818234 - 07/11/20 08:40 PM (3 years, 7 months ago)

When using honey/water to make LC using either spores or from liquid culture how much spores or LC should be injected into 150 ml honey/water ?


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InvisiblebodhisattaMDiscordReddit
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Re: Making Liquid Cuture? [Re: pitriot]
    #26818240 - 07/11/20 08:41 PM (3 years, 7 months ago)

None.


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Offlinepitriot
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Re: Making Liquid Cuture? [Re: bodhisatta]
    #26818288 - 07/11/20 09:03 PM (3 years, 7 months ago)

Can you explain


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OfflineA.k.aM
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Re: Making Liquid Cuture? [Re: pitriot]
    #26818301 - 07/11/20 09:18 PM (3 years, 7 months ago)

Contams will explode in lc, it’s a perfect environment. Spores are rarely clean enough to make lc with, you need to use agar first.


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Invisiblemicelio
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Re: Making Liquid Cuture? [Re: pitriot]
    #26818307 - 07/11/20 09:22 PM (3 years, 7 months ago)

Have you tried agar...?

If not, It would be a good starting point.

In my opinion I wouldn't even consider Lc's until you learn working with agar.

You don't use spores to noc. up Lc jars.


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Invisiblemicelio
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Re: Making Liquid Cuture? [Re: micelio]
    #26818322 - 07/11/20 09:36 PM (3 years, 7 months ago)

Josex' Poke: A different way of doing LC.

https://www.shroomery.org/forums/showflat.php/Number/24740168


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Offlinepesa
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Re: Making Liquid Cuture? [Re: pitriot]
    #26818403 - 07/11/20 10:43 PM (3 years, 7 months ago)

1. LC of any kind its 0.4% of any type of sugar you add.

2. Honey has melting point of 50 °C/122 °F while dextrose/ glucose 146c which is better. but if you are going with honey use a tea spoon. mainly honey clouds the solution and you can not see much what is growing.

3. PC for 15 minutes or put it in boiling water from hobo tek

4. does not matter how much spore you add throw 10ml or 2ml does not make a difference.

5. starting a LC with spore is very difficult mainly keeping solution of lc sterilize during inoculation.

6. people made lc with last 1ml of spore solution got success and fail too.

7. there is a old hobo tek from 14 years does it work sort of you have 100% chance or zero depends on how sterilize you can go.

https://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=5238137&fpart=1&PHPSESSID=

8. lc now days is made with clean agar. if you can start a culture on agar and there is no contams you can transfer that to lc but experimenting with spores does not hurt.

9. you spore print contains million of spores and when you scrap off can not really tell how many you are putting so does not make difference.


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OfflineA.k.aM
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Re: Making Liquid Cuture? [Re: pesa]
    #26818855 - 07/12/20 07:46 AM (3 years, 7 months ago)

If you’re going to use spores it’s best to use as little as possible to minimize the chance of adding contamination with the spores.


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InvisiblebodhisattaMDiscordReddit
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Re: Making Liquid Cuture? [Re: A.k.a]
    #26818944 - 07/12/20 08:57 AM (3 years, 7 months ago)

You don't use spores to start LC. That's a waste of spores. An added step, which is an added chance of contamination.


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Offlinepitriot
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Re: Making Liquid Cuture? [Re: bodhisatta]
    #26819061 - 07/12/20 09:59 AM (3 years, 7 months ago)

Why is it an added step, if I put it first to agar it would be same amount of steps.
If I inject spores directly into grain it takes much longer to colonize.


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OfflineItsaMeShroomio
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Re: Making Liquid Cuture? [Re: pitriot]
    #26819106 - 07/12/20 10:19 AM (3 years, 7 months ago)

You can clean a contaminated culture on agar, you can't clean an LC. The agar step reduces chances of contamination, if done correctly.

Going spores to LC to grain will colonize faster, but will also contaminate faster and with a higher likelihood. An LC, especially with occasional swirling, is an ideal environment for bacteria to proliferate. Myc generally spreads faster than bacteria on a still media like grain or agar, but in liquid it will be everywhere.

If you really want to do LC, I second the recommendation of starting on agar and using the Josex poke to inoculate the LC.


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InvisiblebodhisattaMDiscordReddit
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Re: Making Liquid Cuture? [Re: ItsaMeShroomio]
    #26819109 - 07/12/20 10:20 AM (3 years, 7 months ago)

If you're willing to do the extra steps amd do an assembly line rather than a grow  here and there then start spores on agar not LC. Otherwise skip all that extra step shit all together and uses spores directly to cakes


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Offlinepitriot
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Re: Making Liquid Cuture? [Re: A.k.a]
    #26819121 - 07/12/20 10:29 AM (3 years, 7 months ago)

why is LC so prone to contamination? And is there away to tell if your LC is contaminated or not?


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OfflineItsaMeShroomio
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Re: Making Liquid Cuture? [Re: pitriot]
    #26819164 - 07/12/20 10:51 AM (3 years, 7 months ago)

Because it's liquid and constantly moving, bacteria which do not move or spread fast on their own will spread to all parts quickly and form many expanding colonies. You can't see contamination in an LC unless it's extreme.


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OfflineDoyledozo
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Re: Making Liquid Cuture? [Re: ItsaMeShroomio]
    #26819216 - 07/12/20 11:14 AM (3 years, 7 months ago)

Spores to agar... Expand and transfer until clean. Small sample to lme broth. Test on agar. Trust me. I've made all the mistakes and grown far too many moldy grain jars. Agar is your friend and only seems like an extra step until you see how much time and material you waste halfassing an LC. Pasty has an LC Tek that you can use right in a no pour agar container.


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OfflineDoyledozo
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Re: Making Liquid Cuture? [Re: Doyledozo]
    #26819222 - 07/12/20 11:20 AM (3 years, 7 months ago)



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Invisiblesandman420
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Re: Making Liquid Cuture? [Re: pitriot]
    #26819404 - 07/12/20 01:06 PM (3 years, 7 months ago)

Quote:

pitriot said:
why is LC so prone to contamination? And is there away to tell if your LC is contaminated or not?




The main way to tell visually, LC is cloudy it is probably contaminated. It is normal to be cloudy during/after shaking it though, for LME based LCs during the beginning.

All these folks saying don't put spore on LC because they done did it and ruined 50 jars once or 6 times I'd listen to em.


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OfflineDoyledozo
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Re: Making Liquid Cuture? [Re: sandman420]
    #26819481 - 07/12/20 01:53 PM (3 years, 7 months ago)

My last LC was pure mold. Looked awesome but colonized super fast. That was my first clue. Tested on agar... Straight up green


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Offlinepitriot
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Re: Making Liquid Cuture? [Re: Doyledozo]
    #26820250 - 07/12/20 10:47 PM (3 years, 7 months ago)

How long does it take for a test on agar? I have 4 jars of LC and I am going to need to test them for contam.


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OfflineA.k.aM
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Re: Making Liquid Cuture? [Re: pitriot]
    #26820587 - 07/13/20 07:26 AM (3 years, 7 months ago)

3-5 days when I did it.

For myc at least, idk might be faster if it’s contaminated.


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