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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
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Questions about using NO Verm layer with SHIP
#26817154 - 07/11/20 11:51 AM (3 years, 7 months ago) |
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So here is a picture of the lids I decided to make, Thought it was a pretty good idea for a noob like myself. Credit to Shea25 for the idea I will be using BRF recipe with these jars as I do not find myself advanced enough to try anything different at the moment. Eventually though 

I know that with SHIP you don't have to use a dry verm layer as the jar will not be opened and has my installed SFD. However, I would like to know a few things.
1) Since no verm layer will be used, how much should I fill the jar with my BRF mix? Almost to the top? Or should I still leave 1/2-1 inch space at the top?
2) Should spores be injected directly into the substrate and pressed down against the glass? Or would it be better to have no contact with the substrate and just drip it on top?
3) I know shaking the jar with BRF is a no go so I won't be doing that. But I intend to put foil over them for PC and once inoculated, Would it be a good idea to put it back over the jar or just leave it off since I have a SFD?
4) (Semi-related) But once I feel comfortable enough with sterile technique, What should be my next big step after I have used this setup 3-4 times? Grains? And obviously I would need to make different lids. I have read about 50 different lid teks and would like to know which ones you guys recommend?
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,680
Loc: Central hemisphere
Last seen: 12 minutes, 1 second
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Re: Questions about using NO Verm layer with SHIP [Re: lookintolearn]
#26817167 - 07/11/20 12:02 PM (3 years, 7 months ago) |
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Leave a little space like 1/2 an inch atleast so the mycelium doesn't touch the lid and cover the holes. If your flaming your needle and iso wipe the ship ports, then just inject it down so in can spread rather than just squirt it on top.
No need for the foil after the pc, if you like you can add dust caps over each jar like a coffee filter and elastic band but it's not necessary.
I'd recommend your next step when your comfortable with this, to get a SAB and delve into agar work, maybe even start with cloning a mushroom from one of your Brf grows
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 2 months, 6 days
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Re: Questions about using NO Verm layer with SHIP [Re: Kmacmo]
#26817184 - 07/11/20 12:09 PM (3 years, 7 months ago) |
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Thanks for the quick reply mate I figured that leaving space would be best I just like to have confirmation from someone more knowledgeable.
On the topic of flaming the needle, it's best to flame in between each jar, correct? Or could I get away with maybe two jars (8 ports) between flaming? And so injecting into the BRF would be best you say, That doesn't cause any vector for contams because of the flamed needle correct?
And glad to hear no re-foiling because that just sounds tedious 
I do have a SAB I intend to do this work in but yes agar does sound like the next logical step. Pasty's agar tek seems easy enough. Although with cloning something do you mean taking spore print and making LC/syringes? Or?
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,680
Loc: Central hemisphere
Last seen: 12 minutes, 1 second
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Re: Questions about using NO Verm layer with SHIP [Re: lookintolearn]
#26817369 - 07/11/20 01:23 PM (3 years, 7 months ago) |
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Great, if your using the SAB could just flame between jars or if not using the SAB I'd flame between every injection. Your correct flaming the needle steralizes it and safe to stick in.
Cloning would be taking a mushroom and putting a piece of its flesh onto an agar plate and it will grow out mycelium. Now when you inoculate it's all the same genetics. Spore printing is like collecting seeds, all different genetics. Pastys agar tek will work great there are many other methods also.
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