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Offlineaerow.thefox
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spore hydration
    #26816958 - 07/11/20 10:21 AM (3 years, 6 months ago)

so im trying to start an old print. i was suggested to create a spore syringe. i have done so but wondering how long to leave it to hydrate. figured a week would be fine right? moving to starting on agar after hydration to clean it up and prep it for use.


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InvisibleRoger Clemency
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Re: spore hydration [Re: aerow.thefox]
    #26816972 - 07/11/20 10:29 AM (3 years, 6 months ago)

A week sounds good. I'd also try swiping that print if anything is left. Even if it's old it might do well and I always have trouble with spore syringe to agar.


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Offlineaerow.thefox
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Re: spore hydration [Re: Roger Clemency]
    #26816982 - 07/11/20 10:34 AM (3 years, 6 months ago)

i have tried swabbing straight to agar. with wetter and heavier nutrient mixes too. more than 6 weeks and nothing. re-hydrating in a syringe is the best hope i have right now.  :/


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OfflineKmacmo
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Re: spore hydration [Re: aerow.thefox]
    #26817158 - 07/11/20 11:53 AM (3 years, 6 months ago)

What is your agar recipe?
I recommend trying, 7g agar +7g LME + 500ml water for germination plates. It's what I use and had old spores and fresh spores do well with it.
Just seems easyer to put the spores to agar rather than into a syringe to wait a week then to agar.

I would swipe some to a bunch of plates and make a couple syringes, depending how much spores there is might even have leftovers :laugh:


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InvisibleBro_exotic
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Re: spore hydration [Re: Kmacmo]
    #26817174 - 07/11/20 12:06 PM (3 years, 6 months ago)

I had a 3 year old LGT print, and I bloody destroyed my agar trying to transfer it. Tore it to shit, and it took a couple weeks to do anything, but in the end it came out clean. I made an LC with it and now I've got 7 oat jars colonizing with it. It's possible I just got lucky, but it is possible to use really old prints on agar and have success.


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InvisibleRoger Clemency
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Re: spore hydration [Re: aerow.thefox]
    #26817213 - 07/11/20 12:20 PM (3 years, 6 months ago)

Quote:

aerow.thefox said:
i have tried swabbing straight to agar. with wetter and heavier nutrient mixes too. more than 6 weeks and nothing. re-hydrating in a syringe is the best hope i have right now.  :/




Roger that. I hope it be blowing up soon. If not I can send you a print once I have some more, unless you're specifically trying to grow whatever variety it is.

All my PE6 were sporeless or just didn't really drop much but I have some TOC that should be pinning soon.


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Offlineaerow.thefox
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Re: spore hydration [Re: Roger Clemency]
    #26817604 - 07/11/20 03:22 PM (3 years, 6 months ago)

i have a burma culture going right now. spore syringe to agar, 3 xfers then tic-tac-toed into a jar. that was G2G to 6 more. now im about ready to spawn in a few days. and i have a decent collection tho they all have some age.  and yeah these are redboys. trying to get these specifically to start.

also my current agar recipe is just karo. im just getting back into things after years away and dont have all my supplies yet. alsoholding off while catching up since techniques have advanced while i was away. :tongue:


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