|
Mr.Christmas
Who?



Registered: 04/08/20
Posts: 119
Loc: USA
Last seen: 13 hours, 59 minutes
|
Agar Transfer Question [Video]
#26815253 - 07/10/20 01:49 PM (3 years, 7 months ago) |
|
|
Hi y'all. Hope everyone's having a rad day.
--------------------
Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.
|
polaritymind
relaxed attention


Registered: 10/10/16
Posts: 994
Loc: Germany
Last seen: 3 months, 27 days
|
Re: Agar Transfer Question [Video] [Re: Mr.Christmas]
#26815316 - 07/10/20 02:15 PM (3 years, 7 months ago) |
|
|
Strange format You dont take from the center but more from the rim, the part that has grown the farthest, from what I understand.
-------------------- "to affirm life is to also affirm death" -Albert hofmann
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 24 days
|
Re: Agar Transfer Question [Video] [Re: Mr.Christmas]
#26815390 - 07/10/20 02:52 PM (3 years, 7 months ago) |
|
|
You want to take from the very outer edge of those colonies, which are all the circles you see. So look and find what you think is the best nice cleanest looking one, and then cut a tiny piece from the edge of it. You can take several pieces from different circles and colonies. You're then going to take that tiny piece of edge you cut and put it on a whole new blank agar plate to let it grow out.
--------------------
|
Mr.Christmas
Who?



Registered: 04/08/20
Posts: 119
Loc: USA
Last seen: 13 hours, 59 minutes
|
|
Awesome! Thank y'all!
--------------------
Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.
|
ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 2 months
|
Re: Agar Transfer Question [Video] [Re: Mr.Christmas]
#26815544 - 07/10/20 04:19 PM (3 years, 7 months ago) |
|
|
I like the video. If i knew where you lived I would steal your roll of parafilm. I have to order it by the meter Also we use the same ziploc baggies :
With a plate like that, its pretty overgrown already, but pick a bigger colony and take a tiny (rice grain sized) wedge and drop it to a fresh plate. If a plate looks like that, then contaminates that are present (you dont know at this stage) might be hidden anywhere under that myc. so a good batch of transfers is in order. Once you see those grown out with around 2-3cm radial growth, post some pics and do leading edge transfers of those. going more than 2-3 transfers is only truly necessary for cleaning up a suspect culture or isolating further. I usually end up doing at least 4 transfers before hitting grains.
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
|
Mr.Christmas
Who?



Registered: 04/08/20
Posts: 119
Loc: USA
Last seen: 13 hours, 59 minutes
|
|
Quote:
ManifoldPrime said: I like the video. If i knew where you lived I would steal your roll of parafilm. I have to order it by the meter Also we use the same ziploc baggies :
With a plate like that, its pretty overgrown already, but pick a bigger colony and take a tiny (rice grain sized) wedge and drop it to a fresh plate. If a plate looks like that, then contaminates that are present (you dont know at this stage) might be hidden anywhere under that myc. so a good batch of transfers is in order. Once you see those grown out with around 2-3cm radial growth, post some pics and do leading edge transfers of those. going more than 2-3 transfers is only truly necessary for cleaning up a suspect culture or isolating further. I usually end up doing at least 4 transfers before hitting grains.
Hahah thanks! I'm going to start using parafilm but I had messed up and not used anything to seal my dishes. I lost 5 of em, but these survived so I'm babying em. Yeah, I'm going to probably end up doing 4 transfers so I have a real solid strain. Do you not recommend me throw these in grain once the agar is fully Inocculated? I don't want to have any waste.
--------------------
Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.
|
ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 2 months
|
Re: Agar Transfer Question [Video] [Re: Mr.Christmas]
#26816435 - 07/11/20 03:16 AM (3 years, 7 months ago) |
|
|
If you dont want any waste, dont waste grain by putting inoculate you arent 100% sure of and turning it into neon soup.
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
|
Mr.Christmas
Who?



Registered: 04/08/20
Posts: 119
Loc: USA
Last seen: 13 hours, 59 minutes
|
|
Quote:
ManifoldPrime said: If you dont want any waste, dont waste grain by putting inoculate you arent 100% sure of and turning it into neon soup.
Lol neon soup. Well that makes sense hahahah.
--------------------
Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 113 topic views. 22 members, 195 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|