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OfflineForevergreen
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Registered: 06/23/20
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Last seen: 3 years, 5 months
No inoculation loop. How to use scapel prints to agar
    #26814019 - 07/09/20 11:50 PM (3 years, 6 months ago)

Just heat the scalpel, scoop spores and try to streak? On agar


Edited by Forevergreen (07/09/20 11:52 PM)


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OfflineEntheoGod
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Re: No inoculation loop. How to use scapel prints to agar [Re: Forevergreen]
    #26814023 - 07/09/20 11:54 PM (3 years, 6 months ago)

I’ve read of people dipping the scalpel in clean agar then swiping a bit of spores onto the sticky scalpel blade but that worries me because you could germinate spores that are left on the print and not used because of the leftover agar on the print after you swipe it.

I’d say you’d be fine flaming the scalpel then giving it time to cool and doing what you said if that’s your only option. Could you pressure cook some cotton swabs to use ?


Edited by EntheoGod (07/09/20 11:55 PM)


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Offlineredhandmat
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Registered: 05/09/19
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Re: No inoculation loop. How to use scapel [Re: Forevergreen]
    #26814027 - 07/09/20 11:55 PM (3 years, 6 months ago)

Quote:

Forevergreen said:
Just heat the scalpel, scoop spores and try to streak?




Well you can flame scalpel, cool it, then scrap some spores and streak it. But to make an loop you just need a little bit of wire and a pin or something to attach it to (to hold). There is no way you can't make that. Like you'll find wire anywhere (old cables, pens, ...)


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OfflineMH5109
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Re: No inoculation loop. How to use scapel [Re: redhandmat]
    #26814034 - 07/09/20 11:57 PM (3 years, 6 months ago)

I used a fork with all the prongs cut off except one.


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OfflineForevergreen
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Registered: 06/23/20
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Re: No inoculation loop. How to use scapel [Re: redhandmat]
    #26814045 - 07/10/20 12:05 AM (3 years, 6 months ago)

Quote:

redhandmat said:

Well you can flame scalpel, cool it, then scrap some spores and streak it. But to make an loop you just need a little bit of wire and a pin or something to attach it to (to hold). There is no way you can't make that. Like you'll find wire anywhere (old cables, pens, ...)




Sorry man I don't have anything to make a loop out of besides guitar strings.

Quote:

MH5109 said:
I used a fork with all the prongs cut off except one.




Okay so my scalpel will be fine. Thanks


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Invisiblebudmanman
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Posts: 17,975
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Re: No inoculation loop. How to use scapel [Re: MH5109]
    #26814046 - 07/10/20 12:06 AM (3 years, 6 months ago)

I use a wet pressure cooked q tip lol


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Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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OfflineForevergreen
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Registered: 06/23/20
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Re: No inoculation loop. How to use scapel prints to agar [Re: EntheoGod]
    #26814047 - 07/10/20 12:07 AM (3 years, 6 months ago)

Quote:

EntheoGod said:
I’ve read of people dipping the scalpel in clean agar then swiping a bit of spores onto the sticky scalpel blade but that worries me because you could germinate spores that are left on the print and not used because of the leftover agar on the print after you swipe it.

I’d say you’d be fine flaming the scalpel then giving it time to cool and doing what you said if that’s your only option. Could you pressure cook some cotton swabs to use ?




I didn't think of cotton swabs. I'll try those next time. Thanks homie!


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OfflineForevergreen
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Registered: 06/23/20
Posts: 173
Last seen: 3 years, 5 months
Re: No inoculation loop. How to use scapel [Re: budmanman]
    #26814049 - 07/10/20 12:08 AM (3 years, 6 months ago)

Quote:

budmanman said:
I use a wet pressure cooked q tip lol




I'll look up the process. How do you do it? Wet q tip, pressure cook then swipe?


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Invisiblebudmanman
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Re: No inoculation loop. How to use scapel [Re: Forevergreen]
    #26814054 - 07/10/20 12:12 AM (3 years, 6 months ago)

Yeah I wet the q tip, put itnin a quart jar and pressure cookie for 30 minutes and swipe it over the spores to pick them up and then swipe it on the agar. It always worked for me.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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Invisiblebudmanman
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Registered: 02/07/07
Posts: 17,975
Loc: PNW
Re: No inoculation loop. How to use scapel [Re: budmanman]
    #26814058 - 07/10/20 12:13 AM (3 years, 6 months ago)

You miight not even need to wet it. I just always did.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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OfflineEntheoGod
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Registered: 03/06/15
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Re: No inoculation loop. How to use scapel [Re: Forevergreen]
    #26814079 - 07/10/20 12:35 AM (3 years, 6 months ago)

Not a problem. Glad I could help! :smile:

budmanman's idea sounds great, especially if you dont want to store the swab afterwards. I just read recently you could cut the tip off a spored swab with a sterile pair of scissors or your scalpel and dab it around in the agar with a pair of sterile tweezers. (Flame the tip of the tweezers)


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Offlinesangreen
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Re: No inoculation loop. How to use scapel [Re: budmanman]
    #26814081 - 07/10/20 12:35 AM (3 years, 6 months ago)

For my recent run, I used an 18ga needle (sterile out of the package, opened and uncapped in the SAB, one per print, two plates per print)- swiped the side of the point across the print a few times, then gently wiped it across the surface of the agar- no streaking, I'm trying to gently embed the spores in the agar to give them maybe a better chance to germinate (they've been stored at room temp for a bit over 2 years).


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I figured it out, what life is about
It crawled into my brain and it's starting to sprout


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