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shroomshine
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Syringe dry after PC run
#26813561 - 07/09/20 07:07 PM (3 years, 6 months ago) |
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good evening mushroom folks!
today I PC'ed three syringes (filled with water, needle on, cap on and wrapped in tin foil) together with my "LC to be" for 30ish min @ 15 psi. I let steam out for about 7 minutes before putting the weight on and waited all the way before opening it. there was still plenty of water in the PC. two syringes turned out almost completely dry and the third one was missing half of the water. It is all good since I still had more than enough to proceed. however I would like to avoid this in the future. any tips, tricks, comments?
thanks
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ROTIFIER
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Re: Syringe dry after PC run [Re: shroomshine]
#26813567 - 07/09/20 07:13 PM (3 years, 6 months ago) |
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Yo shroomshine are you trying to prep spore/spawn syringes?
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Crackatoa
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Re: Syringe dry after PC run [Re: ROTIFIER] 1
#26813569 - 07/09/20 07:15 PM (3 years, 6 months ago) |
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I'm assuming it was the quick pressure change when you removed the weight before it was ready.
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shroomshine
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Re: Syringe dry after PC run [Re: Crackatoa]
#26813575 - 07/09/20 07:17 PM (3 years, 6 months ago) |
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No, I am poking agar plates to inoculate LC.
I didn't remove the weight until the pressure was 0 for over 30 min or so
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Pastywhyte
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Re: Syringe dry after PC run [Re: shroomshine] 1
#26813580 - 07/09/20 07:20 PM (3 years, 6 months ago) |
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You need to put the syringes in a jar of water if you don’t want them to boil off in the PC.
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Crackatoa
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Re: Syringe dry after PC run [Re: shroomshine] 1
#26813583 - 07/09/20 07:20 PM (3 years, 6 months ago) |
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Sorry I read that wrong, my fault. You can try the boiling water method of making clean syringes.
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shroomshine
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Re: Syringe dry after PC run [Re: Pastywhyte]
#26813594 - 07/09/20 07:26 PM (3 years, 6 months ago) |
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there you go! thanks pasty! saving my day as always
@crack: I think I am too paranoid to do so I feel the need to flush it with alcohol than boiling water than fill with distilled water than PC
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LogicaL Chaos
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Re: Syringe dry after PC run [Re: shroomshine]
#26813600 - 07/09/20 07:28 PM (3 years, 6 months ago) |
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PCing syringes is overkill. Its much simplier to do the stovetop boiling water technique and uptake-eject boiling water into/out of the syringe 5-6 times then let it cool. Then its ready for use.
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shroomshine
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after dealing with all sorts of contam this past months I am all about the over killing (for now) lol thanks for the advise tho
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ItsaMeShroomio
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If it boiled empty, the problem is probably that you didn't have it needle-side-up. Put them in an a cup inside the PC with the needle side pointing up, and you won't lose it all. Or, wrap the needle separately and cap the syringe.
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shroomshine
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you are right, I just threw them all in there. good tip! thanks!
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MH5109
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Quote:
LogicaL Chaos said: PCing syringes is overkill. Its much simplier to do the stovetop boiling water technique and uptake-eject boiling water into/out of the syringe 5-6 times then let it cool. Then its ready for use.
Meh, I pc my syringes when im doing lc or agar so then I got two or three of them in my half gallon jar. When I want to make transfers pull out one of the sterile packs and open the syringe up. Not saying that way wouldn't work I'm really sketchy on using syringes for spores.
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LogicaL Chaos
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Re: Syringe dry after PC run [Re: MH5109]
#26813654 - 07/09/20 07:49 PM (3 years, 6 months ago) |
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Quote:
shroomshine said: after dealing with all sorts of contam this past months I am all about the over killing (for now) lol thanks for the advise tho
I bet its your spores. Are u using agar? Hows your SAB sterile technique?
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shroomshine
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you nailed it! it is/was my spores I thought the spore syringes I order will be for sure cleaner than everybody else .... hmmm NOPE! lol I did start out with agar a month or so ago and it works great for me! now I want to start with LC so I can produce more spring fresh spawn <3 sterile technique is on point tho! used to work in a lab, that comes in handy now
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LogicaL Chaos
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Re: Syringe dry after PC run [Re: shroomshine]
#26813703 - 07/09/20 08:16 PM (3 years, 6 months ago) |
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Right on! Also try a drop of Lysol in your syringe (use a petri dish to mix). Ive had good sucess with it when i had contaminated spore prints.
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shroomshine
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nice! I will have to try this! thx!
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panne cyanne
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Re: Syringe dry after PC run [Re: shroomshine]
#26813760 - 07/09/20 08:40 PM (3 years, 6 months ago) |
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i use industrial glass syringes separated and loaded in jars with loose lids, and then also a stand alone water water flask with a hole in the rubber cork . i often use an instapot for this. assemble, suck water , ready to go.
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