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OfflineMr.Christmas
Who?
I'm a teapot


Registered: 04/08/20
Posts: 117
Loc: Colombia
Last seen: 12 days, 22 hours
The Healthiest BRF Cakes
    #26813196 - 07/09/20 03:45 PM (3 years, 6 months ago)



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Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.


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InvisibleROTIFIER
Invisible(ish)
Other


Registered: 12/09/19
Posts: 58
Re: The Healthiest BRF Cakes [Re: Mr.Christmas]
    #26813464 - 07/09/20 06:16 PM (3 years, 6 months ago)

Sucio, your link no workio?


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OfflineMr.Christmas
Who?
I'm a teapot


Registered: 04/08/20
Posts: 117
Loc: Colombia
Last seen: 12 days, 22 hours
Re: The Healthiest BRF Cakes [Re: ROTIFIER]
    #26813610 - 07/09/20 07:31 PM (3 years, 6 months ago)



What about this


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Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.


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InvisibleROTIFIER
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Registered: 12/09/19
Posts: 58
Re: The Healthiest BRF Cakes [Re: Mr.Christmas]
    #26813649 - 07/09/20 07:46 PM (3 years, 6 months ago)

Not good dude.. I think you got Trich.. :eek:


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OfflineCrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 29 days
Re: The Healthiest BRF Cakes [Re: ROTIFIER]
    #26813655 - 07/09/20 07:50 PM (3 years, 6 months ago)

Those look green


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:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:



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InvisibleROTIFIER
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Registered: 12/09/19
Posts: 58
Re: The Healthiest BRF Cakes [Re: Mr.Christmas]
    #26813681 - 07/09/20 08:05 PM (3 years, 6 months ago)

From reading your other posts your not having much luck by the look of it. You may have to look over your sterile procedures and clean your environment.

You need to isolate and clean your spore to agar transfers a few more times then take a tiny rice grain sample with sterile procedure to the centre of  a few more dishes and let them grow out before your final check for contams and method of transfer to cakes

Hope that helps keep at it man you'll get their


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InvisibleThe Fresh PrintsS
Smell ya later
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Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: The Healthiest BRF Cakes [Re: ROTIFIER]
    #26813693 - 07/09/20 08:11 PM (3 years, 6 months ago)

He's doing BRF cakes. You don't need to do all that for successful cakes.


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Edited by The Fresh Prints (07/09/20 08:12 PM)


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InvisibleROTIFIER
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Registered: 12/09/19
Posts: 58
Re: The Healthiest BRF Cakes [Re: The Fresh Prints]
    #26813709 - 07/09/20 08:18 PM (3 years, 6 months ago)

True I unerstand ya! but keeping clean as possible makes life a lot easier no?

If you read over all his previous posts you can see he keeps getting contams

What you reckon?


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InvisibleROTIFIER
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Posts: 58
Re: The Healthiest BRF Cakes [Re: Mr.Christmas] * 1
    #26813756 - 07/09/20 08:37 PM (3 years, 6 months ago)

Sucio are you able to take and upload some close up pics at all?


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OfflineMr.Christmas
Who?
I'm a teapot


Registered: 04/08/20
Posts: 117
Loc: Colombia
Last seen: 12 days, 22 hours
Re: The Healthiest BRF Cakes [Re: ROTIFIER]
    #26814198 - 07/10/20 03:06 AM (3 years, 6 months ago)

These have been out for a while without any moisture or light. Was supposed to Chuck em out but I forgot for a while, came back to them today and they looked like this. Thought it was funny

But this was before I started doing agar, this was my 4th round of cakes. After the second round I only had half bad batch and it was because of a contaminated syringe. I just switched to agar because it seemed more fun.


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Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.


Edited by Mr.Christmas (07/10/20 03:08 AM)


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Offlinew00tmycelium
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Registered: 11/28/19
Posts: 159
Last seen: 3 years, 4 months
Re: The Healthiest BRF Cakes [Re: Mr.Christmas]
    #26814206 - 07/10/20 03:21 AM (3 years, 6 months ago)

Quote:

Sucio said:
These have been out for a while without any moisture or light. Was supposed to Chuck em out but I forgot for a while, came back to them today and they looked like this. Thought it was funny

But this was before I started doing agar, this was my 4th round of cakes. After the second round I only had half bad batch and it was because of a contaminated syringe. I just switched to agar because it seemed more fun.




I've had some really horrible syringes in the past, but those cakes look horrendous lol.  That's why I switched to agar.  I swear the quality these days has gone way down on vendor but either way, it's cheaper/quicker to figure out which syringe is legit or not by starting on agar.

Even worst case if you don't feel comfortable with A2G transfer, you can at least test the syringe on agar with a few drops/spores and if it seems like the real thing you can just do the normal process and throw the agar away if you want.  (Or toss in the fridge for later culture/transfer.)


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OfflineMr.Christmas
Who?
I'm a teapot


Registered: 04/08/20
Posts: 117
Loc: Colombia
Last seen: 12 days, 22 hours
Re: The Healthiest BRF Cakes [Re: w00tmycelium]
    #26815050 - 07/10/20 12:20 PM (3 years, 6 months ago)

Quote:

w00tmycelium said:
Quote:

Sucio said:
These have been out for a while without any moisture or light. Was supposed to Chuck em out but I forgot for a while, came back to them today and they looked like this. Thought it was funny

But this was before I started doing agar, this was my 4th round of cakes. After the second round I only had half bad batch and it was because of a contaminated syringe. I just switched to agar because it seemed more fun.




I've had some really horrible syringes in the past, but those cakes look horrendous lol.  That's why I switched to agar.  I swear the quality these days has gone way down on vendor but either way, it's cheaper/quicker to figure out which syringe is legit or not by starting on agar.

Even worst case if you don't feel comfortable with A2G transfer, you can at least test the syringe on agar with a few drops/spores and if it seems like the real thing you can just do the normal process and throw the agar away if you want.  (Or toss in the fridge for later culture/transfer.)




I have a lab at work and recently they've been cleaning up useless machinery and letting the workers bid on it to take it home. There's this sick glove box that's needs a little love but works perfectly. Gonna try convincing them they don't need it anymore lol.


--------------------


Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.


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InvisibleSunny Skies
Cluster Head
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Registered: 05/03/17
Posts: 421
Loc: my house
Re: The Healthiest BRF Cakes [Re: Mr.Christmas]
    #26815178 - 07/10/20 01:19 PM (3 years, 6 months ago)

Keep it simple boss..just use a SAB..GB have a bad rep round here.


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OfflineMr.Christmas
Who?
I'm a teapot


Registered: 04/08/20
Posts: 117
Loc: Colombia
Last seen: 12 days, 22 hours
Re: The Healthiest BRF Cakes [Re: Sunny Skies]
    #26815290 - 07/10/20 02:02 PM (3 years, 6 months ago)

Quote:

Sunny Skies said:
Keep it simple boss..just use a SAB..GB have a bad rep round here.



Quote:

Sunny Skies said:
Keep it simple boss..just use a SAB..GB have a bad rep round here.





I've heard hahahaha. I blame my glove box for a few jack-ups I've done but this is a lab grade glove box like such



I think it'd be rad to at least give it a try.


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Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.


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