I have prepared 2.25 liters of beer which was microwaves for 15 minutes. That is 3 x 750 ml beers.
I have diluted it with 4.5 liters of water boiled in a kettle, in a large plastic water container.
The beer and water was poured into the large container using an old funnel, in open air in decidedly very moldy house.
I have poured 500 ml into each of the bottles pictures in the crate: most of the bottles are old 2L soda bottles. There are two smaller bottles, but all the bottles contain 500 ml of this 1 beer: 2 water ratio. I have done this on the partially enclosed patio during a large storm this day, where there was a molded bowl of oats I had neglected to eat several weeks ago
I have inoculated 4 bottles and marked the (bottle) caps X, with a liquid culture which was created many months ago and stored in the fridge, from a few stem flakes from very dry mushrooms. I am not sure how many mushrooms these flakes come from as they where broken in the baggy I got from the dealer..., so it may not be 1 clone, but a mix of a few clones (like say, 5 or 6, I'm not sure.... This first "master liquid culture" was 1 part beer, and another 4 parts water, and is very pale, far paler than the new 500 ml bottles as seen in the crate. Yesterday I noticed perfect small "blobular" matrices of floating mycelia like tiny jelly fish without tentacles, which as per my experience, denotes that indeed the dry mushroom mycelium, as is mentioned by Roger Rabbit elsewhere in these forums, has been resuscitated by being rehydrated. My dealer had given me some bits of mushroom stems, and I had summarily tossed it in a little jar of water that was boiled (in open air this was put in), which I proceeded to put in the fridge since last November. It seems that the culture, even if contaminated, is still viable, not only after drying, but also after being returned to water and months in the fridge, after which it has also stood now for over a month outside the fridge in autumn and winter at room temperature. I do not have a high def. camera to take a pic.
The other four bottles' caps are marked by a single - stroke, denoting spores from a sporesyringe which had been scraped off a spore print and had been placed in "sterile water" many months ago and kept at room temperature, i.e. transluscent water with tiny bits of black spores or clumps of spores, floating around.
The other four bottles in the middle: two are now inoculated with a 1 part beer 4 part beer LC which was inoculated with same aforementioned sporewater. It is marked by three linear marks, III. It [the LC uced to inoculate the bottles] colonized over a month ago, and a small patch of trichoderma had formed on the top. However a week or two later this trichoderma disappeared, without my shaking it, and it APPEARS (I know, I know), that it is not contaminated, as if the cubensis has achieved some type of species dominance (prolly not but the trich-patch that floated on top is gone, it was about the size of the tip of a pinky).
The other two bottles in the middle, the bottle caps are marked III with a line crossing over the three III, as if crossed out. I have inoculated them with some liquid from the mushroom flakes that have been suspended in water for many months, first in the fridge, and now over a month or so, at room temperature in autumn. These mushroom flakes are not suspended in a beer dilution, but only water. Currently these two bottles have been inoculated in open air by simply pouring the liquid on the patio, on the very sample moldy table (the wood is moldy) where the molded bowl of oats had stood for several weeks.
Remember the first four bottles on the side, with the bottle caps marked I only, where inoculated from another culture in a lighter beer dilute from this water + dry mushroom flake jar, from this very same jar. I have a bunch of bottles of also 1 part beer, 4 parts water diluted and inoculated from this rescucitated mushroom stem flakes in water sample, and they are all colonizing, albeit very little by little, I am not sure if there is not enough nutrients due to the low 1 beer: 4 water ratio, or the cool room temperatures due to winter... I will take a pic of them in a week or two if they seem to be more promising. At first I could see the filaments of mushroom stem and it appeared for a week or so nothing had grown, and I thought I'd kept it the no longer dry mushroom flakes in water without nutes and in the fridge also, too long, and after for another month at room temp., but now again the ephemeral floaty little blobs of mycelium suspended in the solution suggest that it indeed has worked, but not sure if the cold and low beer:water ratio is holding it back. Letting nature be natural (no temp control)...
I will get back in a week or two to take pics of how these cultures look, as the beer is quite transparent and a yellow-orangy gold in colour (again, 1 part beer, 2 parts water, for a total of 3 parts.
I will upload the pics in a few moments but first want to inoculate the last four bottles, and post this before something happens on my computer.
I am not sure if everyone here is familiar with the TV blender tek, but it has a grow rate of complete colonization after 50-72 hours, here is a link from Mycotopia in a pdf on shroomery:
https://files.shroomery.org/attachments/7893959-blenderTek_TVGuide.pdf
I suggest you may want to download this file for convenient referncing.
However, I will detail the nature of my improvisation on this method as I continue my experiment. I will not elaborate now, but am only writing down my sloven procedures. I am not being overly sterile as you have heard, it has been done in open air in a dirty house filled with contams. I have my reason for doing this which I will clarify later on.
Refer here for the pics. Sorry for the low quality.
Again, there is a specific reason I am being so neglectful of sterile procedure, it is in relation to the idea behind the TV Blender tek which I linked to earlier (the pdf on files.shroomery)...
I will elaborate as we approach the next step as the liquid cultures have become sufficiently colonized in my opinion.
A link to the pictures. https://imgur.com/a/9fnN0xC
Edited by PeterPumpkinEater (07/09/20 05:24 AM)
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I am intentionally doing it this way. I understand what you are saying. Did you look at the link I provided to the PDF?
I realise that it is not working.
But my goal you ask?
I want to be able to use bulk liquid culture as, instead of the PF tek recipe of:
2 parts verm 1 part water 1 part BRF
I want to use
2 parts verm 1 part thoroughly colonized LC 1 part BRF.
That is my goal
IN AN OPEN, FILTHY AIRED ENVIRONMENT. I know it will probably fail, but that is specifically what this tek is setting out to prove, the possible (I know I know lol) success of the combination of those two variables.
I will keep on making variations and logging my findings though. I think the beer was too caramelized possibly and is too dilute, I am uncertain. But previously when I made beer LC I had giant globular masses of cubensis mycelium floating about. It is not present here, and although 2 of the bottles have contammed, the others do not yet appear to have.
Literally 10 days minus 1 hour ago.
They're not being incubated either but they're jammed into my burglar bars to get some warm sunshine... for now in the otherwise cold winter here, unincubated otherwise. And by cold I mean around 10 Celsius as "very cold".
And, it is BASED on the TV-Blender tek's principles, which is detailed in the PDF, which was a MAJOR success. However what is important for me are other variables' concomittant syncreticism (asterile, sloven procedures).
However sloven I may be, the parameters are meticulously noted, odd as that may seem. I am just trying to achieve some type of species dominance.
Please take a gander at the actual TV-tek and share your opinion. But yes, this is NOT a tek, but is just referencing one that worked quite marvelously, by its very own supposed account.
Again for our perusal: https://files.shroomery.org/attachments/7893959-blenderTek_TVGuide.pdf
Edited by PeterPumpkinEater (07/19/20 04:25 AM)
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