|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 2 hours
|
This is why you you need to put spore syringes to agar for grain spawn. 1
#26810200 - 07/08/20 02:32 AM (3 years, 7 months ago) |
|
|
 I usually use prints but I have a Ps. Galindoi ATL-7 spore syringe from a trusted source.
It's always hard to get the first drop out. I ended up squirting a couple drops on my first plate, and even though I tried to swipe it with a loop it still rolled around a bit.
You can see up around 12 - 1 o'clock I marked where the mold is. The circled spot with the arrow is where I took my transfer, it was grown over by the time I took the photo this morning.
It's dark now because the mold sporulated but it started off as a white dot, pretty indistinguishable from the myc germination . This also illustrates why swiping with a loop is so important - if I was able to swipe properly my germination would be in the zig zag pattern and I would avoid anything outside of it because it might be a mold colony. Bacteria looks like this:

Since the mold hadn't sporulated yet I used the Flow Hood and turned the plate so that the 12 o'clock spot was downwind in the flow from where I was making my transfer from. I think I caught it early enough to make a transfer. I'll update with a photo of the next plate once it fills out more, so far so good. If the mold had sporulated already and I wanted to try for a transfer, I would have had to do it in a still air box. Too risky for a FH. Had I injected this into my grain jars, even with just those 3 or 4 drops it would have been contaminated with mold.
This is the beauty of agar, it allows us 2-D surface to identify clean mycelium and transfer it away to a reciever plate.
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (07/08/20 04:49 AM)
|
LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,597
Loc: The Inexpressible...
Last seen: 1 hour, 47 minutes
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: mushpunx]
#26810248 - 07/08/20 03:45 AM (3 years, 7 months ago) |
|
|
An excellent demostration of why Agar should always be used when working with spores and grain
|
Forevergreen
Stranger
Registered: 06/23/20
Posts: 173
Last seen: 3 years, 5 months
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: LogicaL Chaos]
#26810276 - 07/08/20 04:13 AM (3 years, 7 months ago) |
|
|
So ive had plenty of myc and not mold. Wtf this hobby is fucking mindblowingly difficult. I cant tell what is mold or myc. Ive nocced over 100 dishes with spores and just did like 40 jars of oats.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 2 hours
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: LogicaL Chaos] 1
#26810291 - 07/08/20 04:31 AM (3 years, 7 months ago) |
|
|
Thanks LC!
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 2 hours
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: Forevergreen]
#26810300 - 07/08/20 04:44 AM (3 years, 7 months ago) |
|
|
Quote:
Forevergreen said: So ive had plenty of myc and not mold. Wtf this hobby is fucking mindblowingly difficult. I cant tell what is mold or myc. Ive nocced over 100 dishes with spores and just did like 40 jars of oats.
What's the problem you are having? Do you use a loop when you inoculate with spores? It makes it easier to identify clean myc - bacteria is easy to spot (unless it's thin film, then it can be tricky) but mold colonies can be especially hard to pick out - when you streak, at least you can avoid anything outside the zig zag pattern.
I usually just inoculate two plates when I am starting from spores. Like in the case of this Galindoi here, there is the plate I posted above where I'm reasonably sure the two transfers I took from will be clean. It germinated pretty quickly so I waited a little longer than usual to take my transfers. The second plate I inoculated finally just germinated yesterday and I'm taking a transfer today.
--------------------
 Amateur Mycologists United AMU Q&A
|
Forevergreen
Stranger
Registered: 06/23/20
Posts: 173
Last seen: 3 years, 5 months
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: mushpunx]
#26810305 - 07/08/20 04:51 AM (3 years, 7 months ago) |
|
|
Look in my link. I keep thinking i have cobweb mold then people tell me its myceliu but not cubes.
I opened this one and smudged it with my finger because people said yes to myc and mold. I think it was myc. It was fluffy af
Edited by Forevergreen (07/08/20 04:53 AM)
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 2 hours
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: Forevergreen]
#26810324 - 07/08/20 05:08 AM (3 years, 7 months ago) |
|
|
Quote:
Forevergreen said: Look in my link. I keep thinking i have cobweb mold then people tell me its myceliu but not cubes.
I opened this one and smudged it with my finger because people said yes to myc and mold. I think it was myc. It was fluffy af

How are you wrapping your plates, can I see a photo of one of yours wrapped?
I've never seen cobweb mold on a plate. I've only seen it once ever actually - it super thin and whispy, like an actual cobweb, has a greyish look to it.
--------------------
 Amateur Mycologists United AMU Q&A
|
Forevergreen
Stranger
Registered: 06/23/20
Posts: 173
Last seen: 3 years, 5 months
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: mushpunx] 1
#26810328 - 07/08/20 05:13 AM (3 years, 7 months ago) |
|
|

Im pretty convinced I not only wasted that plate but have myc. A lot of it is wispy, but its all white.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 2 hours
|
Re: This is why you you need to put spore syringes to agar for grain spawn. [Re: Forevergreen] 1
#26811126 - 07/08/20 02:53 PM (3 years, 7 months ago) |
|
|
Here is a photo of this plate a few days after it germinated. I haven't worked with Galindoi in a couple years so I was unsure of it at first :

But now that it's grown out a bit after I took transfers, I can see that it was definitely clean myc (first pic below). The mold on the other side looked almost identical at first and now you can see how green those spots are:

Now here is the other plate I inoculated from that same syringe , it finally showed some growth yesterday morning. The Circled spot is *possibly* Galindoi myc but it's really suspect. The two spots to the side of it looked identical at first and now it is fairly obvious they are mold. I had placed a single drop of solution on the plate and swiped with a loop. There is no evidence of the swipe, it seems I might not have gotten any myc germination on this plate at all, just the mold.

Just goes to show how easy it is to mistake mushroom + mold myc,and how both mold, mushroom spores + bacteria can all be in the same "clean" syringe. Not the end of the world though, because we have agar. The two transfers I took from the first plate look clean so far.
--------------------
 Amateur Mycologists United AMU Q&A
|
|