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Offlinedark-goblin
Stranger


Registered: 01/16/20
Posts: 13
Last seen: 1 year, 9 months
Resurrecting 6+y/o spores with fresh mycelial growth * 1
    #26811032 - 07/08/20 01:49 PM (3 years, 6 months ago)

Forgive the somewhat clickbaity title, but that's pretty much the thrust of what I'm getting at here. I couldn't find any info on this, and I'm interested in the thoughts of this community, especially those with advanced mycological knowledge.

Some quick context: I recently tried to germinate 6+ year old spores on agar, with no luck. The spores were sealed and well preserved, and I completed the procedure with decent sterile technique. That was back in Feb. Since then, the spores have just been sitting there, in the SAB, with absolutely no signs of growth (not even visible invasive molds).

I realise the common knowledge is that spores this old are exponentially harder to get going, but it was an experiment I was interested in carrying out.

So, after ~5 months I'm fairly certain that these spores are not going to germinate in this setup. Maybe they're completely hopeless - but again, it's an experiment.

Which brings me to my current question (TL;DR):

What would happen if I transplanted a small section of fresh, healthy, vital mycelium onto an old spore dish?

Some thoughts:
  • Would there be any chance of all of this new mycelium 'picking up' the spores, maybe activating some kind of latent germination potential?
  • Would the spores just be lost in the new growth, with no effect whatsoever?
  • Has anyone ever tried this in an experimental context/recorded any interesting results?


Thanks
Based on my mycological knowledge, I predict the following outcome:
You may choose only one


Votes accepted from (07/08/20 04:43 AM) to (No end specified)
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Invisiblenooneman
Male

Registered: 04/24/09
Posts: 14,555
Loc: Utah
Re: Resurrecting 6+y/o spores with fresh mycelial growth [Re: dark-goblin] * 1
    #26811065 - 07/08/20 02:06 PM (3 years, 6 months ago)

Spores have been known to occasionally survive in syringes for 10 years, but it probably depends heavily on how they were stored. You might try adding the old spores to a syringe with water, and waiting a day or two, then trying to use the syringe on agar or something. The spores might be dehydrated/need water. Alternatively, it might be possible that only a handful are still alive, so you might need to try using all the spores to find the couple that survived. Or they may all be dead.

The new mycelium is likely to grow regardless of if the spores are dead or alive, so there'll be no way to tell what effect if any the spores had on the mycelium.


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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 3 months
Re: Resurrecting 6+y/o spores with fresh mycelial growth [Re: nooneman] * 1
    #26811083 - 07/08/20 02:20 PM (3 years, 6 months ago)

I revived 8 year old spores doing that, put a drop on agar every couple days. Mine must've been very dry because it took 10 days of soaking to get them to grow. Resulting tubs were average or slightly above.


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OfflineThe shroomy 1
Luminous beings surround me
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Folding@home Statistics
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 4 days
Trusted Cultivator
Re: Resurrecting 6+y/o spores with fresh mycelial growth [Re: Rapjack] * 1
    #26816361 - 07/11/20 01:11 AM (3 years, 6 months ago)

I'm about to try and revive an 11 year old print .  I've had success with a 7 year old print..


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AMU Q&A thread.


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Offlinethe man
still masked
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Registered: 08/12/99
Posts: 6,681
Loc: C A N A D A
Last seen: 2 hours, 49 minutes
Re: Resurrecting 6+y/o spores with fresh mycelial growth [Re: The shroomy 1] * 1
    #26833527 - 07/20/20 02:20 AM (3 years, 6 months ago)

10 yo prints here took more time for hydration no sense adding spores to myc unless ur doing a cross with venom. by the time they germ the myc will have eaten all surface nutrients


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And Moses Said "Let my mushrooms grow!"


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