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Shop: Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate, Cultivation Supplies

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Offlinejajajshshdj132
Stranger
Registered: 05/21/19
Posts: 3
Last seen: 3 years, 6 months
Multiple failure with spawn production. Seeking advice.
    #26810995 - 07/08/20 01:33 PM (3 years, 6 months ago)

Hi guys.

I do Agar. My Agar dishes fruit successfully, and my contam rate is below 10% regarding Agar.

My spawn on the other hand - is not doing so well. So i really seek your advice!!

So!

I'm doing spawn bags T3 - approx 2,5kg
I use Organic Rye
Boil until saturated
Sterilize for 3 hours in Presto 23
inoculate with 100% clean agar, or LC.

I've done experiments with using bottled water, and water from different locations as i thought this might be it. No.

I've done 100+ bags, with very little success.

My contam rate with spawn bags are ... +90%


Edited by jajajshshdj132 (07/08/20 01:34 PM)


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OfflineSpirit-Crusher
DOOM HIPPIE


Registered: 04/24/19
Posts: 358
Loc: PNW
Last seen: 15 days, 13 hours
Re: Multiple failure with spawn production. Seeking advice. [Re: jajajshshdj132]
    #26811014 - 07/08/20 01:39 PM (3 years, 6 months ago)

Are you packing your presto full? I think you can squeeze 6 bags in there with approx 5lbs in each. I had much better luck when I sized down to 4 bags per pressure cooker.

Bags are a bitch.


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Offlinejajajshshdj132
Stranger
Registered: 05/21/19
Posts: 3
Last seen: 3 years, 6 months
Re: Multiple failure with spawn production. Seeking advice. [Re: Spirit-Crusher]
    #26811020 - 07/08/20 01:41 PM (3 years, 6 months ago)

Thank you so much for your input!
Ill even try one fucking bag at a time just if it brings me some success!


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OfflineBikerfool
Your Local Edgelord
Male

Registered: 11/21/05
Posts: 1,577
Last seen: 5 months, 12 days
Re: Multiple failure with spawn production. Seeking advice. [Re: jajajshshdj132]
    #26811043 - 07/08/20 01:56 PM (3 years, 6 months ago)

Contams can easily pass through the filter on bags if it's wet. We would need a lot more information to figure out where the problems is. Bags are more challenging to work with. I would try jars with a synthetic filter disc.

You say you don't think it's the agar. Contams can hide in a plate that looks "good." And if 10% of the plates are dirty it's possible you're missing some.

There's so many other ways for a bag to contam. Are you inoculating in front of a flow hood? I wouldn't use bags without one.


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Just an angsty teen contributing to the pubs decline with contentless posts.


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Offlinejajajshshdj132
Stranger
Registered: 05/21/19
Posts: 3
Last seen: 3 years, 6 months
Re: Multiple failure with spawn production. Seeking advice. [Re: Bikerfool]
    #26811058 - 07/08/20 02:03 PM (3 years, 6 months ago)

Thank you for your response.

What other information do you need?

My filters are never wet and grains never touch the filter.

Even if 10% of the agar dishes were carrying a contam - it would be hard to make 90% fail based on 10% contaminated dishes.

I am not inoculating in front of a flow hood - i use a large SAB, but as stated, for every 100 dishes i make 10% or less fail.

I do have a flow hood, but actually found the SAB was better.


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OfflineTweeq
Tweeq of Nature
Male

Registered: 06/07/18
Posts: 2,043
Loc: Netherlands Flag
Last seen: 6 hours, 43 minutes
Trusted Cultivator
Re: Multiple failure with spawn production. Seeking advice. [Re: jajajshshdj132]
    #26811089 - 07/08/20 02:26 PM (3 years, 6 months ago)

Why don't you try jars too? if all the bags go south..


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Multiple failure with spawn production. Seeking advice. [Re: Tweeq]
    #26811105 - 07/08/20 02:36 PM (3 years, 6 months ago)

Jars are a much more forgiving way to start to get into grain. I would recommend finding some success with them first before trying to move up to bags.


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Offlinemetaphoric
Mr. Bater
Male User Gallery


Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
Re: Multiple failure with spawn production. Seeking advice. [Re: Pastywhyte]
    #26811154 - 07/08/20 03:08 PM (3 years, 6 months ago)

Jars for sure. They are much more forgiving as pasty puts it


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OfflineMycobro420
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Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Multiple failure with spawn production. Seeking advice. [Re: Pastywhyte]
    #26811182 - 07/08/20 03:27 PM (3 years, 6 months ago)

I had a problem with bags.and I also have a 23qt presto I did 4 bags with 5qts of rye did a bunch of them g2g with a qt of spawn each they all fully colinized the bags. Never shook them (if I did I would have know they were bad). Put spawn to 66qt monotub some fully colinized some only 50% got flushes off most but all got trick.i figured since I stuffed PC and wraped bags in sheets the cores of the bag didn't get fully sterlized.so since then I have Ben running 4qt bags 4 at a time and they are doing fine.so I think under sterlized


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