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OfflineEnkidu
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Put these plates to grain?
    #26810456 - 07/08/20 06:54 AM (3 years, 6 months ago)


Hard to see in the pic but there are a few spots on the plates that look like they might be contam

When i hold it to the light i can see them, but not sure its contam or air bubble. I think some are contam

Considering cutting out the clean portions and putting to grain

I got 20 plates i just made transfers to hoping to get some clean ones, but theres def aome clean myc on here i could cut out and put to grain instead of waiting

What would you do?


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InvisibleLadysKnight
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Re: Put these plates to grain? [Re: Enkidu]
    #26810476 - 07/08/20 07:05 AM (3 years, 6 months ago)

Quote:

Enkidu said:

not sure its contam or air bubble. I think some are contam


What would you do?




I'd keep transferring clean myc til you're sure you have clean cultures.


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OfflineSoupyGeorge
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Re: Put these plates to grain? [Re: Enkidu]
    #26810494 - 07/08/20 07:17 AM (3 years, 6 months ago)

:whathesaid: 


Quote:

Enkidu said:
What would you do?




I have an extremely limited experience in this hobby as I have only been working with agar long enough to make two transfers, but my educated opinion would be to let them grow out more. The amount of mycelium on they plate would just take a while to colonize. Even just 3 more days on the agar and it will grow out a good bit more giving a significant difference in your jars colonization time.

Day of transfer 6/30/20:


3 days of growth:


7 days of growth:


These are going to grain tonight. I only posted pictures so you can see how fast it could cover your agar and how it would give your grains more inoculation points once you shake up the jar with the introduced agar. I believe patience now will pay off in your colonization time for the jar.


Edited by SoupyGeorge (07/08/20 07:19 AM)


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OfflineEnkidu
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Re: Put these plates to grain? [Re: SoupyGeorge]
    #26810566 - 07/08/20 08:18 AM (3 years, 6 months ago)

Guess ill wait for these new plates to grow out


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InvisibleLadysKnight
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Re: Put these plates to grain? [Re: SoupyGeorge]
    #26812398 - 07/09/20 09:09 AM (3 years, 6 months ago)

Quote:

SoupyGeorge said:
:whathesaid: 


Quote:

Enkidu said:
What would you do?




I have an extremely limited experience in this hobby as I have only been working with agar long enough to make two transfers, but my educated opinion would be to let them grow out more. The amount of mycelium on they plate would just take a while to colonize. Even just 3 more days on the agar and it will grow out a good bit more giving a significant difference in your jars colonization time.

Day of transfer 6/30/20:


3 days of growth:


7 days of growth:


These are going to grain tonight. I only posted pictures so you can see how fast it could cover your agar and how it would give your grains more inoculation points once you shake up the jar with the introduced agar. I believe patience now will pay off in your colonization time for the jar.




Hey bro, that last pic shows myc at 11 o'clock, the rest of it is mold.


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OfflineEnkidu
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Re: Put these plates to grain? [Re: LadysKnight]
    #26812423 - 07/09/20 09:24 AM (3 years, 6 months ago)

I didnt even pay attention to the plates and he said theyre going to grain

Nice catch


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InvisibleLadysKnight
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Re: Put these plates to grain? [Re: Enkidu]
    #26812437 - 07/09/20 09:34 AM (3 years, 6 months ago)

Would have helped to say something earlier, I know. But just saw his reply today, been busy.


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OfflineSoupyGeorge
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Re: Put these plates to grain? [Re: LadysKnight]
    #26812498 - 07/09/20 10:04 AM (3 years, 6 months ago)

Quote:

LadysKnight said:
Would have helped to say something earlier, I know. But just saw his reply today, been busy.




At this moment in time I disagree. This photo from my phone is quite blurry, I assume (dangerous I know) that you believe it is cobweb mold. I have had the fuzzy white mold get a few plates, IME it showed up on a different area of the plate and grew much faster than the mycelium. On this plate in question all originated from the center transfer piece (which could have been contaminated with the mold itself hence the mold originating from the center if you are correct) but it all grew at the same steady pace and shows no separation between the mycelium and the mold. I've seen clean plates where the rizomorphic growth is just stronger in some sectors and that is what I believe is the case with this plate. All of this comes from a hope in my heart and little to no knowledge. You may be 100% correct, but I hope not. Either way I will still have two jars to use for G2G so all hope is not lost.


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I look like everyone else so that i don't stand out to the man.
We are not the same.


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InvisibleLadysKnight
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Re: Put these plates to grain? [Re: SoupyGeorge]
    #26812514 - 07/09/20 10:12 AM (3 years, 6 months ago)

I'm not advanced enough to say which mold it is, but I have seen mold come in all speeds. But you're right, it could be a blurry piic or the lighting or a variety I'm not familiar with, I have been wrong before.
Good luck sir!


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OfflineEnkidu
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Re: Put these plates to grain? [Re: LadysKnight]
    #26813271 - 07/09/20 04:37 PM (3 years, 6 months ago)

Looked pretty questionable to me when you mentioned it

Maybe Bod or someone else could jump in and ID if it is or not


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