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OfflineStarBoyAni
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Registered: 05/03/20
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Last seen: 3 years, 6 months
Agar with Calf Brain and Beef Heart Infusion
    #26810071 - 07/07/20 11:32 PM (3 years, 6 months ago)

So I got a bit excited and ordered 20 sterile pre-made nutrient agar petri dishes online, without checking what exactly was in them.

Turns out they have calf brains and "beef heart" infusion, as well as peptone, dextrose, sodium chloride and disodium phosphate.

Will mycelium grow in this agar?

Just my luck too - I went into the local asian mart today and found telephone brand agar-agar powder. Will definitely be making my own BRF agar.

Thanks guys! :grin::mushroom2:


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OfflineJus
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Registered: 09/27/19
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Re: Agar with Calf Brain and Beef Heart Infusion [Re: StarBoyAni]
    #26810187 - 07/08/20 02:17 AM (3 years, 6 months ago)

Check this thread out!

Some interesting recipes in there, including beef broth.
In general, as long as it's got nutrients and water, mycelium will grow there, baring any other obvious factors like anti-fungal ingredients.


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Offlinemushpunx
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Registered: 04/20/14
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Re: Agar with Calf Brain and Beef Heart Infusion [Re: StarBoyAni]
    #26810188 - 07/08/20 02:18 AM (3 years, 6 months ago)

The Telephone agar is great man, it's what I use.

It might possibly grow because of the peptine and dextrose. Is there any way to return them? If you could I'd return them and just get some plates since you can pour your own now.

BRF agar is fine. I'd suggest MEA or PDA tho. Light malt extract is cheap, just a few bucks for a pound that will last a long time. You just weigh out your agar and LME and boom , done.
You can get LME online or at any brewing store. If you can't get that just use instant mashed potato flakes. For PDA you wanna add in some dextrose, you can use a spoonful of Karo syrup too.


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Amateur Mycologists United
AMU Q&A


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