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OfflineMikefalopio
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Unidentified black dots contamination on my first monotub
    #26809584 - 07/07/20 06:01 PM (3 years, 6 months ago)

Sorry if my English is not so good, here is the thing: I started this whole process like 45 days ago. I received an agar plate from a friend, which has what i later learned to be tomentose growth; anyways, i inoculated 4 pints and 4 brf cakes with it. I think the colonization was good because i didn't notice any kind of contamination during it. The problem came when i put them to fruit, first the cakes, which i had put into a SGFC, started to show black pins until they got covered on them. I said, "screw it, i fucked up these" so i tossed them away. Then a couple of days later i spawned a monotub with the jars, I used 3 jars of 500cc each. The colonization went smoothly but when i put them to fruit, again i had these black pins. My question is: could the agar be my source of contamination even if the jars didn't show anything strange during colonization? Or does this mean that the contamination happened at another stage? I've done some research and i can't find anything that looks like these black dots.

Here are some pics of the agar I used and the contam itself:



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OfflineA.k.aM
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Re: Unidentified black dots contamination on my first monotub [Re: Mikefalopio]
    #26809596 - 07/07/20 06:09 PM (3 years, 6 months ago)

That plate is off, definitely bacterial. Im surprised the cakes colonized so well.


The growth on the agar should be smooth and fluffy, with the little hairs visible.



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OfflineMikefalopio
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Re: Unidentified black dots contamination on my first monotub [Re: A.k.a]
    #26809614 - 07/07/20 06:19 PM (3 years, 6 months ago)

Yeah, that's what i suspected, i thought it could be this tomentose growing, but considering is the same contamination in both the cakes and the monotub makes sense that the agar is off.


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OfflineMikefalopio
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Re: Unidentified black dots contamination on my first monotub [Re: A.k.a]
    #26809623 - 07/07/20 06:24 PM (3 years, 6 months ago)

Do you think i can get some shroom out of the tub to try and clone it or is it ruined?


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Invisiblesandman420
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Re: Unidentified black dots contamination on my first monotub [Re: Mikefalopio]
    #26809665 - 07/07/20 06:44 PM (3 years, 6 months ago)

This is not bacteria, some kind of white mold mycoparasite or viral disease. Bacteria looks like grease on agar not mushroom type mycelium, and you will be able to see colonies. Maybe mycogone wet bubble, it causes pins to rot. But you would usually see a lot of amber droplets and blobs with that.

The original agar plate is contaminated. You would be better off throwing these thing away asap and cleaning your room and destroy all your cultures.


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Edited by sandman420 (07/07/20 06:49 PM)


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Offlinepolaritymind
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Re: Unidentified black dots contamination on my first monotub [Re: Mikefalopio]
    #26809669 - 07/07/20 06:47 PM (3 years, 6 months ago)

Is that cubensis we're talking about here? That mycelium on the plate looks a little unsual, more like a gourmet or something. Those colonized tubs look normal though.
Not sure what those are, at first I thought aborts but they're kinda too small for that. Its not clear to me that its a contam or which, I'd wait it out until it becomes more pronounced so you can tell easier. Might be smal specks of black mold or fungus gnat eggs but dont put too much weight into these, neither I have ever seen and these are just crazy theories.
Let's wait for one of the experts to chime in :wink:
But yeah another guess is you were scammed or a mistake happened and this mycelium from some other mushroom, which just happens to grow well on the same substrates?? Nah that doesnt add up. It cant be all mold mycelium that woulda sporulated long ago. So yeah, maybe some strange hidden bacterial infection or a white mold mycoparasite as was said before? Sounds plausible, never heard of that before though, will admit that


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OfflineMikefalopio
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Re: Unidentified black dots contamination on my first monotub [Re: sandman420]
    #26809675 - 07/07/20 06:51 PM (3 years, 6 months ago)

Actually, i have those amber droplets on my tub! I think i misidentified them as metabolites. Thanks for the info


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Invisiblesandman420
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Re: Unidentified black dots contamination on my first monotub [Re: polaritymind]
    #26809679 - 07/07/20 06:52 PM (3 years, 6 months ago)

I assumed this was cubensis. It could possibly be something else that is a good theory too.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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Invisiblesandman420
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Re: Unidentified black dots contamination on my first monotub [Re: sandman420]
    #26809682 - 07/07/20 06:53 PM (3 years, 6 months ago)

Mycogone perniciosa aka wet bubble then is a solid bet. If the amber droplets are truly oddly only droplet sized, not yellow pooling.

And i do see what could be considered some bubbles in the tub too in there.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (07/07/20 06:55 PM)


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OfflineMikefalopio
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Re: Unidentified black dots contamination on my first monotub [Re: sandman420]
    #26809693 - 07/07/20 06:57 PM (3 years, 6 months ago)

It's supposed to be GT, but at this point idk lol. I was ready to star all over again with a transfer from the same agar, thank god I hesitated.


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OfflineKizzle
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Re: Unidentified black dots contamination on my first monotub [Re: Mikefalopio]
    #26809963 - 07/07/20 09:47 PM (3 years, 6 months ago)

Tomentose mycelium can just be from genetics and the nutrient mix can also affect how it grows but sometimes it harbors contaminants so it's generally a good idea try to get linear growth on agar to use.


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