Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
OfflineSt.Mushrick
Stranger
Registered: 07/07/20
Posts: 4
Last seen: 10 months, 17 days
Rye spawn contamination mystery
    #26809364 - 07/07/20 03:55 PM (3 years, 6 months ago)

Hi guys,
I have a kind of locked room murder mystery type of contamination story for ye. I ordered Cambodian cubensis spore solution with a bag of hydrated and sterilised rye. I inoculated the bag using aseptic techniques. The bag was stored in a hot press (room with the water heater where towels are kept, I don't know what ye call them in other countries. Apologies for being Irish) Monitored with an indoor thermometer between 26 and 29°c at times.
The spawn seemed to colonise fine and now it's showing signs of what I think could either be trichoderma contamination or bruising. Where could the contamination have come from? Should I notify the supplier of the rye or is that even a thing? Forgive me if this is posted in the wrong section I only set up this account to make this post. Huge mycology enthusiast. 

I'll add the screenshots of the video I took if I can.



Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 16 hours
Re: Rye spawn contamination mystery [Re: St.Mushrick] * 1
    #26809402 - 07/07/20 04:15 PM (3 years, 6 months ago)

3 things:

1. Syringes are notorious for being dirty/contaminated. This why most here use agar to clean it before inoculating.
2. The temp is too high. Room temp is perfect for all stages of growth. Warmer temps seem to give contams an advantage over the mycelium.

3. Could be that the grains weren't sterilized properly. This is why we recommend doing it all yourself. If you want something done right, you gotta do it yourself.


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Rye spawn contamination mystery [Re: One of Us]
    #26809420 - 07/07/20 04:27 PM (3 years, 6 months ago)

I accept your apologies for being Irish, it's OK nobodies perfect.

This looks like it was contaminated from either the open top during inoculation or when the guys made the bags, or from incorrect sterilization. Or just simply the seal on the top is not correct. I can see the good mycelium in some chunks so I think the spores were clean enough, but who can really tell just saying what my gut tells me on these.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (07/07/20 04:30 PM)


Extras: Filter Print Post Top
OfflineSt.Mushrick
Stranger
Registered: 07/07/20
Posts: 4
Last seen: 10 months, 17 days
Re: Rye spawn contamination mystery [Re: sandman420]
    #26809515 - 07/07/20 05:20 PM (3 years, 6 months ago)

Thanks guys.
Any suggestions on where to find a guide to prepare my own spawn bags?


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Rye spawn contamination mystery [Re: St.Mushrick]
    #26809528 - 07/07/20 05:27 PM (3 years, 6 months ago)

Yea sure right here

Buy some millet
Put 3lbs of millet in the bag
Put 2lbs water in the bag
Shake the bag around to mix the water in
Let sit 24 hours
Mix around again a bit
Fold top of bag down neatly so that the bag sits on its own top and the gussets are nice and neat
PC for 3-4 hours (you will need to modify your weight to not release steam at 15 psi by taping a few quarters to it. This will make it not lose water)
Let cool down about 3 or 4 hours before opening pc and grabbing the bag by the gussets in a way that doesnt unfurl the bag and set on a shelf to finish cooling 6+ hours
After totally cool you can move to your SAB or hood for inoc


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
OnlineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,489
Loc: Zone 5
Last seen: 27 seconds
Trusted Cultivator
Re: Rye spawn contamination mystery [Re: sandman420]
    #26809703 - 07/07/20 07:02 PM (3 years, 6 months ago)

It is very rare that any spore print is ever 100% free from other organisms (contams). It is not impossible and has been done many times successfully, but putting any spores on grain directly is a crap shoot. It "may" work really well but more often it ends in a nasty colored possibly slimy and stinky fate. Chucked in the compost bin.

Agar work is not really that hard or expensive. You can get 500 sterile plates for 50-80 bucks and that is usually enough for the average hobbyist for a year or so.

Good luck and keep on truckin' on. Eventually you'll find what works for you.

Plants love Trichoderma. My garden is looking great!


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Extras: Filter Print Post Top
OfflineSt.Mushrick
Stranger
Registered: 07/07/20
Posts: 4
Last seen: 10 months, 17 days
Re: Rye spawn contamination mystery [Re: Nichrome] * 1
    #26810297 - 07/08/20 04:39 AM (3 years, 6 months ago)

I'd have no problem working with agar plates. I've a background in science but I haven't heard of any other methods of inoculation that are used. Could you link me a guide? Thanks for all your help!


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 1 hour, 57 minutes
Trusted Cultivator
Re: Rye spawn contamination mystery [Re: St.Mushrick]
    #26810363 - 07/08/20 05:40 AM (3 years, 6 months ago)

As a general rule, never put spores straight to grain. They are too dirty for that, hence the green mold you are seeing.

Search "No-Pour Agar" and you should find some good links. Or u can order pre-made agar plates from various Shroomery sponsors as well.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* rye spawn mistake advice? midas 1,993 5 03/22/18 03:07 AM
by elasticaltiger
* Log of my first time using rye spawn (NEW PICS)
( 1 2 3 all )
jebus2000 10,467 43 07/04/12 04:38 PM
by tomoceusz
* PsC, rye spawn, coco coir... amyloid 1,637 2 05/10/03 05:31 AM
by Demiurge
* Rye Spawn cookiewhore 1,537 5 02/16/02 10:17 AM
by Papa_Bear
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,087 21 04/30/02 03:27 AM
by Zebedee
* Re: ---Making rye spawn in a bag???--- Anonymous 2,342 7 01/28/00 08:01 AM
by Anonymous
* Rye Spawn cookiewhore 1,209 7 02/07/02 04:52 PM
by durban_poison
* Quick rye spawn question. Techniques 1,440 3 06/21/02 07:23 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
141 topic views. 22 members, 162 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.