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Anonymous

Re: Large fruits via multi-spore innoc
    #268085 - 03/08/01 09:05 PM (16 years, 7 months ago)

How exactly does weeding out jars at that early of a stage result in large fruits? Why weed out strains at all, at this stage? I don't use syringes much, unless I have to. But I try to isolate as many strains, variants, as possible. The key is to weed out contaminants, not mushroom strains. Some strains are slower, not worse. How do you judge fruiting potential at this early of a stage? This saves time, just like high inoculation rates do. This doesn't gaurantee large fruits.



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InvisibleMcMan
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Post deleted by users_request [Re: ]
    #268589 - 03/09/01 10:31 AM (16 years, 7 months ago)



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InvisibleMcMan
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Post deleted by users_request [Re: McMan]
    #268597 - 03/09/01 10:56 AM (16 years, 7 months ago)



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OfflineLizard King
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Re: Large fruits via multi-spore innoc [Re: McMan]
    #268624 - 03/09/01 01:27 PM (16 years, 7 months ago)

Great advice Mcman. I've been doing the same thing lately with grain to grain transfers. I hate busting out the agar because I have awful luck with it. The next best thing is to choose a jar out of a batch that was inoculated via multispore that has the best most rhizomorphic growth. Then take a piece of healthy myceluim from that jar and use it to inoculate your next batch of grain, this way you end up with a pure strain(or very close to it) in each of your grain jars. The result is an entire batch of jars(colonized sub) that will all uniformly produce the same results under optimum conditions, except you didn't have to bust out the agar and petris.


Even better yet, you can prepare your favorite grain, but only fill a few jars with about 1/2" of substrate, and drop a piece of mushroom tissue down in it. Allow to colonize, transfer to another jar to isolate from contaminants if needed, and use this to transfer to more grain. Now you have a pure strain that is a clone, which means every bit of mycelium in your jars is capable of fruiting. The results are wall to wall uniform flushes under optimum conditions.



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InvisibleMcMan
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Post deleted by users_request [Re: Lizard King]
    #268634 - 03/09/01 01:51 PM (16 years, 7 months ago)



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Offlinekingkc
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Re: Large fruits via multi-spore innoc [Re: McMan]
    #268656 - 03/09/01 03:01 PM (16 years, 7 months ago)

Very good advice. I've seen something similar in that wedges from a nice sector on a Petri are transferred to smalll amounts of bulk substrate. Those jars are then used as one might use grain to get more mass. This totally removes grain from the picture but it can be successful.



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Anonymous

Re: Large fruits via multi-spore innoc [Re: kingkc]
    #268690 - 03/09/01 04:01 PM (16 years, 7 months ago)

I understand what you are saying, I'm just uncertain that weeding out so many possible strains at an early stage is a good idea. Thick rhizomorphs is your determining factor for selecting the jars you use as your grain masters. This works to establish a viable strain, that grows well in your environmental parameters. It by no means ensures large fruits. This system is definetly better than injecting an entire syringe into one jar, but I'm not convinced your weeding out at this early of a phase is a great idea. I like variety, having as many isolates as possible to test. This insures that I obtain what I'm looking for. Good advice though for someone with limited funds, time , and space. This is a hobby for me. I enjoy the process of culture as much as I do enjoying the fruits of my labor. I certainly spend more time growing then ingesting. Variety is the spice of life.



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InvisibleMcMan
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Post deleted by users_request [Re: ]
    #268706 - 03/09/01 04:49 PM (16 years, 7 months ago)



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Anonymous

Re: Large fruits via multi-spore innoc [Re: McMan]
    #268880 - 03/09/01 10:38 PM (16 years, 7 months ago)

Sorry McMan, didn't mean to be a troll. It was'nt my intent.



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OfflineEddie Walker
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Re: Large fruits via multi-spore innoc [Re: McMan]
    #268912 - 03/10/01 12:08 AM (16 years, 7 months ago)

I had a huge post then I clicked back accidentaly and it dissapeared. Here is the mojoritiy of it rewritten.

I belive that the selection of faster colonizing already domesticated strains could benifit the off spring. However as Mcman, I and all of you are already aware of the only way to truely geneticly select is thru agar cultures. I do belive that McMan has a good idea. If one jar is colonizing faster then the rest it can really only be 2 things enviroment or genetics. In retrospect after I opened my Books 79.95$ up I learned it is more complicated. Sigh...

The Mushroom Cultivator (415 pages) 29.95$ Chapter 2, page 25
Ramifications of Multitispore Culture
In reply to:

Multispore culture is the least difficult method of obtaining a viable if not absolutely pure strain. In the germination of such a multitude of spores, one in fact creates many strains, some incompatible with others each potentially different in the manner and degree to which they fruit under artificial conditions. This mixture of strains can have a limiting effect on total yields, with the less productive strains inhibiting the activity of more productive ones. In general, strains created from spores have a high probability of resembling their parents. If those parents have been domesticated and fruit well under labrotory conditions, their progeny can be expected to behave similarly. In contrast, cultures from wild specimens may fruit poorly in an artificial environment. Just as with wild plants, strains of wild mushrooms must be selectively developed.




It goes on and on for 5 more paragraphs most of it does not apply but I will transcribe the rest if anyone requests. I wish I had a damn scanner. :-)

P.S. I am in no way affiliated with Fungi Perfecti. I am only a satisfied customer. They are an incredible rescource.

Mycology supplies available at Fungi Perfecti .


--------------------
Mycology Supplies[blue]Fungi Perfecti[/blue]


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InvisibleMcMan
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Post deleted by users_request [Re: Eddie Walker]
    #269054 - 03/10/01 10:26 AM (16 years, 7 months ago)



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InvisibleInsomnia
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Re: Large fruits via multi-spore innoc [Re: McMan]
    #269060 - 03/10/01 10:47 AM (16 years, 7 months ago)

In the same vein, I've just made two spore syringes from one humongous Cambo. When pins started forming, I made note of the most agressive and earliest mushroom. I then kept track of it and factored in the size. It finished off 4" tall, very dense, and with a cap almost as large as a silver dollar. An absolute monster from BRF/Verm cased 50/50 in a small tray.

So when I inoculate with these syringes, I'll be conservattive about how much I use in order to find the best performers.

Peace.

__________________________
I choked Linda Lovelace!


--------------------
"If you believe in things you do not understand, you will suffer." ? Stevie Wonder


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