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polaritymind
relaxed attention


Registered: 10/10/16
Posts: 994
Loc: Germany
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: A.k.a]
#26801035 - 07/03/20 08:35 AM (3 years, 7 months ago) |
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Quote:
A.k.a said: lol hell yeah!
I confess I didn’t set my SAB station up right the other day and kept catching myself resting my wrists/forearm on the armholes.
So dumb. It’s looking like around 70% contam rate on the plates so far lol. Gonna have to redo them. The slightest bump of the SAB will knock all kinds of stuff into the air.
Wait why is that a problem, dont really pay attention to it but might have been doing this always and that doesnt change my 95% success rate.
-------------------- "to affirm life is to also affirm death" -Albert hofmann
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A.k.a
Stranger



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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: polaritymind]
#26801042 - 07/03/20 08:39 AM (3 years, 7 months ago) |
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My SAB stays where it is so I end up not cleaning it very often. That and running it dry in an extremely dirty room mean if I don’t do things perfectly they get contaminated. If I mist the walls and floor first it’s not a huge deal but when I’m just doing plates I normally take zero precautions past flaming the blade.
Anyway when it hasn’t been cleaned and it’s dry any contact at all with the SAB seems to kick up clouds of spores.
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LAGM2020     
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doc9151]
#26801111 - 07/03/20 09:21 AM (3 years, 7 months ago) |
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I'm so confused as to why you would do this Doc? There's so much consensus, advice given and dogma about sterile technique involving flaming tools red hot, and sanitising vs sterilising, and I dont see what the benefit to using Iso instead of flaming could be. It even seems like it would be more effort, require 2 hands instead of 1, would take longer to clean and would cost the same (ethanol for my lamp is unreasonably expensive) . The "Willy Myco" flame thread gave that dude shit for flaming then wiping with iso. But at least the iso is hitting hot metal and potentially boiling before touching the sample. Thats why your post confuses me.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: ManifoldPrime]
#26801285 - 07/03/20 11:17 AM (3 years, 7 months ago) |
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Sloppy technique can work just fine, until it doesn’t. I’ve struggled with more than my fair share of contams so if a practice is going to give me a 0.5% better chance of avoiding a vector I’m gonna take it. It all adds up in my experience.
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oculodextro

Registered: 04/10/13
Posts: 1,205
Loc: Space is the Place
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
#26801295 - 07/03/20 11:21 AM (3 years, 7 months ago) |
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My technique is: Give the hands a good wash. Spray the SAB with soapy water. ISO my gloves, always redhot blade between different samples.
Can't remember the last time I actually lysoled or cleaned the box. I know exactly when I'm getting a contam from being stupid or just having dickfingers.
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead] 1
#26801309 - 07/03/20 11:28 AM (3 years, 7 months ago) |
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Quote:
TedsDead said: No problems with iso dripping on ur plates and retarding growth? That always happens to me
Not at all, I don't keep the towel dripping wet, only wet enough to be able to squeeze out a little iso when wanted. I will bunch up the paper towel where the blade and handle meets so I can squeeze a little alcohol into the area, but I still have a corner of the paper towel wrapped around the bottom of the handle to catch any extra runoff, I also lay a dry paper towel on the bottom of my sab and work over that area. My Sab is made from the largest tote I could find (3ft long × 2ft w x 1/1/2 ft H) so I have plenty of room for a decent run without being crowded to much.
Someone said something about having concerns about the air flow when putting their hands in and out of the s.a.b., don't worry about it, be more concerned about the placement of your s.a.b. near vents or walls were the air flow is bouncing off the wall and in through the hand holes. I find keeping the hand holes parallel to the direction a vent is blowing and the wall that air will deflect off of across the room will make the biggest difference along with technique inside the s.a.b.
Remember, never allow your hands or arms to to be over top of any sterile areas such as agar plates and grain jars, never allow anything to touch the sides of plates or jars when transferring or innoculating. Having good technique is the most crucial factor in my opinion along with proper sterilization. Mastering technique and sterilization will make all the difference when it comes to contaminants.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
Edited by Doc9151 (07/03/20 11:29 AM)
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bare.whiterabbit
Lapin Blanc



Registered: 11/29/09
Posts: 912
Loc: The Microwave
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doc9151]
#26801869 - 07/03/20 05:23 PM (3 years, 7 months ago) |
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Quote:
Doc9151 said:
Quote:
TedsDead said: No problems with iso dripping on ur plates and retarding growth? That always happens to me
Not at all, I don't keep the towel dripping wet, only wet enough to be able to squeeze out a little iso when wanted. I will bunch up the paper towel where the blade and handle meets so I can squeeze a little alcohol into the area, but I still have a corner of the paper towel wrapped around the bottom of the handle to catch any extra runoff, I also lay a dry paper towel on the bottom of my sab and work over that area. My Sab is made from the largest tote I could find (3ft long × 2ft w x 1/1/2 ft H) so I have plenty of room for a decent run without being crowded to much.
Someone said something about having concerns about the air flow when putting their hands in and out of the s.a.b., don't worry about it, be more concerned about the placement of your s.a.b. near vents or walls were the air flow is bouncing off the wall and in through the hand holes. I find keeping the hand holes parallel to the direction a vent is blowing and the wall that air will deflect off of across the room will make the biggest difference along with technique inside the s.a.b.
Remember, never allow your hands or arms to to be over top of any sterile areas such as agar plates and grain jars, never allow anything to touch the sides of plates or jars when transferring or innoculating. Having good technique is the most crucial factor in my opinion along with proper sterilization. Mastering technique and sterilization will make all the difference when it comes to contaminants.
I dig this advice, man. I was concerned with the whole in and out for flame, which has always been a concern of mine and also eventually shits the metal your flaming over and over sooner or later.
I too am using a 104L for my SAB now which should help with crowding and allow me to have everything I could need at hand. I'll give the iso towel trick a try this first round.
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Camera93
We got dicks like Jesus



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I confess: I poured LC over 6 pints, dropped APE clone wedges to 2 masters, and made several transfers last night....and a not a single pic was taken. Is that any kind of milestone lol, I used to take a pic of the same jar hours apart
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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A.k.a
Stranger



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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Camera93]
#26806296 - 07/06/20 06:46 AM (3 years, 7 months ago) |
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Hahah funny how that happens.
I used to wake up and check out all my jars religiously and now I’ve run enough grows and have so much going on that not only have I stopped monitoring them but I’m finding colonized jars I totally forgot about.
I confess I dropped a plate of this playa uvita clone I was all excited for to four jars the other day, all have green in them already. So somehow the plate looks great but has an aggressive mold in it.
Also I have like 15 jars in my garage that I’m scared to open. Been thinking about throwing them out but can’t do it. Maybe I’ll just get somebody off Craigslist to clean them for a dollar each or something lol.
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LAGM2020     
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: A.k.a]
#26806334 - 07/06/20 07:28 AM (3 years, 7 months ago) |
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Confession #1 Garage Jars... about 25, 10-18 months old, every color of the rainbow ... except white!

Confession #2 A couple of weeks ago I was inoculating spawn bags and I also had a couple half gallon jars in the PC. I went to shake one of those half gallons and it cracked open spilling grain and shards of broken glass all over the inside of my PC and the spawn bags. I kept going, carefully lifting spawn bags out, brushing off spilled grain and hoping there were no micro punctures. They’re looking pretty good. I finished pretty late. This week I prepped grain and started to load up the PC, opened the lid and... the smell!!! I found the two inches of water and two quarts of fermenting grain in the PC, right were I’d forgotten it more than a week ago!
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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3some
Haagse Harry



Registered: 04/20/20
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
#26806350 - 07/06/20 07:43 AM (3 years, 7 months ago) |
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I left a jar of contaminated brown rice grains for a month and finally cleared it last night. My kitchen was literally smoked bombed with green spores when I pour it into the bin.
I have a feeling I have to leave this hobby until I move to a new house.
-------------------- Ask me about growing yeast, mold and bacteria. I'm very experienced. The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: 3some]
#26806360 - 07/06/20 07:55 AM (3 years, 7 months ago) |
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3some Plenty of folks have managed this hobby in old moldy houses. Work on your sterile technique and be mindful of your work spaces. Pro tip... if you have a jar full of spores like that. Pour a sink full of soapy water, agitate it so it has a layer of bubbles on top. Submerge the jar - lid still on, then slowly open the lid far enough so water will flood in. When the air bubbles stop coming up ... wait 5 minutes then pour that green soup in your trash. You’ve just avoided spreading thousands of potential contaminants in your house!
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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3some
Haagse Harry



Registered: 04/20/20
Posts: 278
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
#26806443 - 07/06/20 08:48 AM (3 years, 7 months ago) |
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Quote:
PsiloPsychIn said: 3some Plenty of folks have managed this hobby in old moldy houses. Work on your sterile technique and be mindful of your work spaces. Pro tip... if you have a jar full of spores like that. Pour a sink full of soapy water, agitate it so it has a layer of bubbles on top. Submerge the jar - lid still on, then slowly open the lid far enough so water will flood in. When the air bubbles stop coming up ... wait 5 minutes then pour that green soup in your trash. You’ve just avoided spreading thousands of potential contaminants in your house!
Never thought of that. Should have asked here before doing that. I sprayed the whole area with disinfectant. Wish me luck.
-------------------- Ask me about growing yeast, mold and bacteria. I'm very experienced. The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners
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sangreen
Dunce


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Re: TRUE CONFESSIONS - of an amateur mycologist. Contest Someday I Promise! :} [Re: 3some]
#26807215 - 07/06/20 02:49 PM (3 years, 7 months ago) |
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I confess I'm back. I can't leave this wonderful world forever.
-------------------- I figured it out, what life is about It crawled into my brain and it's starting to sprout
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Camera93] 1
#26807985 - 07/06/20 09:40 PM (3 years, 7 months ago) |
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Quote:
Camera93 said: I confess: I poured LC over 6 pints, dropped APE clone wedges to 2 masters, and made several transfers last night....and a not a single pic was taken. Is that any kind of milestone lol, I used to take a pic of the same jar hours apart
I always trip when I’m doing a growlog that I’m bumping it with too many pics and people gonna be sick of it.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
#26808646 - 07/07/20 09:30 AM (3 years, 7 months ago) |
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I confess, that 3rd RW chocolate WHOOPED MY ASS! Got what I needed from it though... definitely in the right place
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NaudiShroomer
Stranger
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Caps McGee]
#26808691 - 07/07/20 09:57 AM (3 years, 7 months ago) |
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I had 4 jars that that sat for over 3 weeks after inoculation with no growth, was first attempt at rye and it seemed way too dry. So I opened them up and put them under bathroom faucet to get some moisture in there then sealed em back up... 2 weeks later 3 of the 4 are fully colonized and seamiongly unscathed. Still have a long way to go but ive came this far!
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oculodextro

Registered: 04/10/13
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: NaudiShroomer]
#26808703 - 07/07/20 10:07 AM (3 years, 7 months ago) |
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You brave motherfucker! I would of PC'd some water and carefully re-wet in a SAB or under a flowhood.
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Kromazone
Higher than groceries


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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: oculodextro]
#26809485 - 07/07/20 05:08 PM (3 years, 7 months ago) |
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Wow, I just started but my wife is starting to get concerned about my obsession with wanting to inoculated every pp5 container in the dollar store, then the other day when we left Wally be world with 100$ worth Of monos and mason jars/lids she snapped. I don't think she'll ever get it, and she be even grows weed for a living but she still don't get it....
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polaritymind
relaxed attention


Registered: 10/10/16
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Loc: Germany
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kromazone]
#26809575 - 07/07/20 05:56 PM (3 years, 7 months ago) |
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Well does she take them and knows their value, or is she even against the substance. Many people unfortunately have some prejudice. Maybe grow some edibles too, but then again that would take even more equipment and space to not have to reduce you active production and serve them to her. Maybe she likes oysters, king oysters, shiitake etc?
-------------------- "to affirm life is to also affirm death" -Albert hofmann
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