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Offlinemysticalshroom975
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Registered: 07/03/20
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Thoughts on my agar recipe?
    #26807048 - 07/06/20 01:48 PM (3 years, 6 months ago)

I'd like to get your thoughts on my agar recipe.  I didn't have dextrose, so I couldn't go for the well established recipes and had to get creative.  I'm a little concerned I didn't add enough sugars because I couldn't taste any sweet when I tasted it.  The ratios:

20g potato starch
25g maple syrup
20g agar
1L distilled water

Also, does anyone know how long it takes to be able to see growth?  A few drops from a spore syringe (with no spores visible) were used.  Temp is set to 85F.


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Offlineroarkell
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Re: Thoughts on my agar recipe? [Re: mysticalshroom975]
    #26807146 - 07/06/20 02:23 PM (3 years, 6 months ago)

That seems like a lot of sugars to me... roughly 2% potato and 2.5% maple syrup... my standard recipe is 1.5% nutes total... I'm sure you'll get growth but I'd bet it's super slow... also; a single drop from a syringe is enough and 85 is too hot... go 75ish and you'll have much better luck


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Invisiblesandman420
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Re: Thoughts on my agar recipe? [Re: roarkell]
    #26807309 - 07/06/20 03:23 PM (3 years, 6 months ago)

I usually see potato flakes being used at 5 grams per liter.


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Offlinemysticalshroom975
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Re: Thoughts on my agar recipe? [Re: roarkell]
    #26807887 - 07/06/20 08:38 PM (3 years, 6 months ago)

Quote:

roarkell said:
That seems like a lot of sugars to me... roughly 2% potato and 2.5% maple syrup... my standard recipe is 1.5% nutes total... I'm sure you'll get growth but I'd bet it's super slow... also; a single drop from a syringe is enough and 85 is too hot... go 75ish and you'll have much better luck



How long do you think it should take after inoculating with a spore syringe unitl I start seeing mycilium?  And at what rate should it grow?  Then I'll know if it's going too slow.


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Offlineroarkell
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Re: Thoughts on my agar recipe? [Re: mysticalshroom975]
    #26807925 - 07/06/20 09:01 PM (3 years, 6 months ago)

Usually within 3 days I see growth and I keep everything very close to 75 at all times. Some genetics are slower than others, if you haven't seen anything after 7 days or so I'd nocc up some more plates... side note: I used to set the syringe up in a pint jar, needle down, overnight and then drip a few drops on a sterile swab and use that swab to streak plates. Doing it that way I was far more successful with syringe > agar.


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