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OfflineDabrit
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Stalling : Starting to wonder if I have allowed for adequate Gas Exchange
    #26806636 - 07/06/20 10:38 AM (3 years, 6 months ago)

So I inoculated some Rye jars that I had sterilized @15 PSI for 2 hours on June 27th with a clean culture I had grown on agar.

So far this is all the growth I'm seeing :



I'm starting to wonder if the holes I have in the lids are adequate enough for GE. I watched a RR video which I'm pretty sure said to use 3 x 1mm holes in the lids as should be more than enough for gas exchange so that's what I went with. I am using synthetic filter discs as my filter. But so far growth seems to have completely slowed down. Shouldn't I be seeing more growth than this by now?

After a couple of days of inoculation I did shake up the jars a little thinking this would help speed up growth but not much is happening?


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OnlineLogicaL ChaosM
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit]
    #26806643 - 07/06/20 10:40 AM (3 years, 6 months ago)

Its not your filter, its your grain. Its too dried out. Need to make more grain jars and try again. Hopefully u have more colonized agar.


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OfflineJustweed
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit] * 1
    #26806648 - 07/06/20 10:40 AM (3 years, 6 months ago)

Show us the holes on the lids? The jars look too dry which will definitely keep the mycelium from growing much.


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Keep 'em high and tight guys....


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OfflineDabrit
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: LogicaL Chaos]
    #26806660 - 07/06/20 10:44 AM (3 years, 6 months ago)

Quote:

LogicaL Chaos said:
Its not your filter, its your grain. Its too dried out. Need to make more grain jars and try again. Hopefully u have more colonized agar.




But how though? When the grains came out of the pressure cooker there was tons of condensation inside the jars? Is two hours maybe too long to sterilize for? When I prepped the grain I just shook them in their strainers to steam dry and waited until there was no wet spot when doing the toilet paper test .


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OfflineDabrit
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Justweed]
    #26806683 - 07/06/20 10:51 AM (3 years, 6 months ago)

Quote:

Justweed said:
Show us the holes on the lids? The jars look too dry which will definitely keep the mycelium from growing much.




Here is a pic of the size of holes in the lids:



I don't know how my grains could be so dry though as I only allowed the grain to be dry enough to pass the toilet paper test. And just steam dried them in a strainer? I did get a bunch of burst grains though from boiling for 10 minutes at full boil could this perhaps explain things?


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OnlineLogicaL ChaosM
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit]
    #26806686 - 07/06/20 10:52 AM (3 years, 6 months ago)

Two hours is a long time. Im not total certain on how grains dry out like that, but i know they are no good for growing mycelium.


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OfflineJustweed
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: LogicaL Chaos] * 1
    #26806721 - 07/06/20 11:08 AM (3 years, 6 months ago)

2 hours PC'd is a long time. It's possible that you cooked off the moisture of the grains in the jars and what was left in the jars was just moisture clinging to the inside. Kinda like how you can boil chicken for 15 minutes, you cooked it in water but once you take it out it's dry as hell.

Do your jars for 90-95 minutes @15psi. Past that you're just wasting time and cooking the grains more.


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Keep 'em high and tight guys....


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OfflineDabrit
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Justweed]
    #26806730 - 07/06/20 11:11 AM (3 years, 6 months ago)

Quote:

Justweed said:
2 hours PC'd is a long time. It's possible that you cooked off the moisture of the grains in the jars and what was left in the jars was just moisture clinging to the inside. Kinda like how you can boil chicken for 15 minutes, you cooked it in water but once you take it out it's dry as hell.

Do your jars for 90-95 minutes @15psi. Past that you're just wasting time and cooking the grains more.




How do the holes look for the lids?

Ok thanks usually I do 90 minutes but was trying others methods this time around.


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OnlineLogicaL ChaosM
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit]
    #26806735 - 07/06/20 11:13 AM (3 years, 6 months ago)

I typically do 70mins at around 12psi and it works great for me :shrug:


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OfflineJustweed
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit]
    #26807739 - 07/06/20 07:06 PM (3 years, 6 months ago)

Quote:

Dabrit said:
Quote:

Justweed said:
2 hours PC'd is a long time. It's possible that you cooked off the moisture of the grains in the jars and what was left in the jars was just moisture clinging to the inside. Kinda like how you can boil chicken for 15 minutes, you cooked it in water but once you take it out it's dry as hell.

Do your jars for 90-95 minutes @15psi. Past that you're just wasting time and cooking the grains more.




How do the holes look for the lids?

Ok thanks usually I do 90 minutes but was trying others methods this time around.



Not good honestly, I've never seen lids like that...Kinda like that for PF cakes I guess but I dunno where you saw to do that. Typically I do 1 3/8th in hole in the middle of my lids personally. I know others do something different, 2 holes sometimes seems to be another norm.

Yea I personally do 95 minutes @15psi for my corn jars. Only time I get contams is when I forget to put my mask on during G2G.


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Keep 'em high and tight guys....


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InvisibleBsdgaou

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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Justweed]
    #26808098 - 07/06/20 11:26 PM (3 years, 6 months ago)

I am doing 8 jars at the bottom and two bags at the top. 17 PSI, 120 min. They're not dry, mycelium is growing fine on them.


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InvisibleGoatrider
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: LogicaL Chaos]
    #26808138 - 07/07/20 12:05 AM (3 years, 6 months ago)

Perhaps you disturbed the myc by shaking too early.

Also the lid holes won`t be the issue for dry grains.,
That won`t be enough GE for quarts.

There isn`t any condensation on the jars,
so it`s due to grain prep for sure.


Quote:

LogicaL Chaos said:
I typically do 70mins at around 12psi and it works great for me :shrug:




You mean with rye?
Interesting, need to try that.
Never thought that would be enough.

                          :elmo:


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OfflineFuddyduddy
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Goatrider]
    #26808175 - 07/07/20 01:08 AM (3 years, 6 months ago)

I had a similar issue with stalled jars using the same lids as you. When I added extra layers of micropore tape they took off and colonized in days after that.

Worth trying before giving up at least


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OnlineA.k.aM
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Fuddyduddy]
    #26808337 - 07/07/20 06:14 AM (3 years, 6 months ago)

I pc for two hours a lot of times too, you just gotta adjust your grain soak time. Although I use wbs and I’ve heard some grains just turn to mush.


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OfflineDabrit
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Justweed]
    #26817405 - 07/11/20 01:41 PM (3 years, 6 months ago)

Quote:

Justweed said:
Quote:

Dabrit said:
Quote:

Justweed said:
2 hours PC'd is a long time. It's possible that you cooked off the moisture of the grains in the jars and what was left in the jars was just moisture clinging to the inside. Kinda like how you can boil chicken for 15 minutes, you cooked it in water but once you take it out it's dry as hell.

Do your jars for 90-95 minutes @15psi. Past that you're just wasting time and cooking the grains more.




How do the holes look for the lids?

Ok thanks usually I do 90 minutes but was trying others methods this time around.



Not good honestly, I've never seen lids like that...Kinda like that for PF cakes I guess but I dunno where you saw to do that. Typically I do 1 3/8th in hole in the middle of my lids personally. I know others do something different, 2 holes sometimes seems to be another norm.

Yea I personally do 95 minutes @15psi for my corn jars. Only time I get contams is when I forget to put my mask on during G2G.




Roger Rabbit himself made a video saying to only use 3 x 1mm(1/16th) holes in the metal lids when using a synthetic filter disk as a filter and using the metal lid metal side down to the grains. It's in his how to grow mushrooms video collection in the video named "Let's Grow Mushrooms! 1 3 5 Steps to Perfect Grain Spawn":



I mean he's probably the most valued TC on this site so figured he would know better than anyone.

Funny thing is later this week my jars started to grow again and some are now close to 30% colonized. The start of summer has been rather rainy and cool so that might also explain things, I'm also thinking I may have had too many burst kernels from over boiling which means they won't retain as much moisture. But looks like things may pan out and may just take a little longer. I'll G2G with a fresh set of jars once the first one is ready just to make sure I get a full set of jars to grow with.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineDabrit
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Re: Stalling : Starting to wonder if I have allowed for adequate Gas Exchange [Re: Dabrit]
    #26817425 - 07/11/20 01:50 PM (3 years, 6 months ago)

Just took a picture of the jars from today as you can see quite a bit more growth now so who knows why they went dormant the way they did. I may be good now :





--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




Edited by Dabrit (07/11/20 01:55 PM)


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