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Offlinemcnugget
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Registered: 06/22/13
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Bacteria? Competitor mold? A chance to get away?
    #26806465 - 07/06/20 09:06 AM (3 years, 6 months ago)

My Burma culture seems to have been contaminated with something.  I've been trying to transfer away for it for a month with no luck.  While tossing old cultures I noticed this plate.  On the edges it looks like some of the myc outran whatever was holding it back.  If I transfer just those tips I'm hoping I can save it.

Does it look like bacteria or a competitor mold riding along with it?  Whatever it is I think this is my last chance before I go back to spore so wish me luck.





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Offlineredhandmat
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Registered: 05/09/19
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Re: Bacteria? Competitor mold? A chance to get away? [Re: mcnugget]
    #26806504 - 07/06/20 09:32 AM (3 years, 6 months ago)

How many transfers is this? Looks like a T1 or T2 to me... I mean it looks normal, just not far from germination plate. I have seen plates like this and asfaik it wasnt contaminated. just partly rhizo and partly tomentose.


Edited by redhandmat (07/06/20 09:34 AM)


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Offlinemcnugget
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Re: Bacteria? Competitor mold? A chance to get away? [Re: redhandmat]
    #26806534 - 07/06/20 09:48 AM (3 years, 6 months ago)

Quote:

redhandmat said:
How many transfers is this? Looks like a T1 or T2 to me... I mean it looks normal, just not far from germination plate. I have seen plates like this and asfaik it wasnt contaminated. just partly rhizo and partly tomentose.





This is T3 from a plate pin that came from a tissue culture.  The growth from all my transfers starts out looking ideal, then a few days after growth establishes itself it gets extremely splotchy starting from the center out.  The leading edge of growth also gets a greasy looking ring around it which is not present where the tips of the mycelium have outran the surrounding growth


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OfflineLogicaL ChaosM
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Re: Bacteria? Competitor mold? A chance to get away? [Re: mcnugget]
    #26806595 - 07/06/20 10:22 AM (3 years, 6 months ago)

It looks OK to me :shrug: Probably just Rhizomorphic mycelium in my opinion (a good thing).


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