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OfflineLeglocklarry
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Registered: 07/06/20
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Inoculating and incubating agar plates
    #26806314 - 07/06/20 07:09 AM (3 years, 8 months ago)

Following the BOD's useful links page and other resources I did my first agar plate attempt. I've read the ideal temp is something like 28c - 32c? Which is what I've been keeping them at - Most in a sandwich bag, sitting on upside down seedling tray above a warming mat in a grow tent.

They're about 7 days since inoculation - are they colonizing slow?

One pic is an obvious contam. I think I hit 11 out of 12 correct.


Edited by Leglocklarry (07/06/20 07:13 AM)

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OfflineKmacmo
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26806335 - 07/06/20 07:29 AM (3 years, 8 months ago)

Looks good, for 7 days there pretty fast.
25-28 is the ideal temp, 32c is abit high and heat mats can cause problems better off without it


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OfflineLeglocklarry
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Re: Inoculating and incubating agar plates [Re: Kmacmo]
    #26822640 - 07/14/20 09:54 AM (3 years, 8 months ago)

Thanks. I can't really remove the mat because its winter down here and generally it sits at a comfy 28c. In saying that I've got one that has overnight developed a bit of a liquid problem. The rest seem kind of slow in developing after 2.5 weeks but maybe I'm comparing my spore based plates to fruit body plates?

Only a few seem to have some nice, thick white growth.

What should I do with them now? The obvious being transfer pieces to grain, but, should I make more plates from these plates? Is where and how I take pieces of agar important? I saw a video of a bloke going out of his way to cut where two areas were bridging together. Yeah I dunno, kind of lost other than to start making grain.

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OfflineLeglocklarry
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26822646 - 07/14/20 09:58 AM (3 years, 8 months ago)

Seems like some took to the streaking I did. Others developed a single circle, some two circles and at least one is just random looking.

Sorry I don't know correct terminology. Anyone know what the sudden liquid build up is in that first pic and is it still good to use for grain? The mycelium and the agar seem to be fine?


Edited by Leglocklarry (07/14/20 10:00 AM)

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OfflineKmacmo
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26822974 - 07/14/20 12:42 PM (3 years, 8 months ago)

Plate 2,3,6 all look good. (in order of pictures left to right)
You let them grow out too much but they can still be used.
I highly recommend you don't use any of them for grains, instead transfer pieces to a new clean petri plate, If that grows out clean then you can use it on grains.

I can't usefully describe to you where to take transfers from, watch some videos and search around on the shroomery!


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Edited by Kmacmo (07/14/20 01:01 PM)

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OfflineLeglocklarry
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Re: Inoculating and incubating agar plates [Re: Kmacmo]
    #26824215 - 07/15/20 12:39 AM (3 years, 8 months ago)

Thanks man I appreciate your feedback. I only have a couple of plates left so I will try some A2A, be back with a noob question or two in a few days.

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OfflineKmacmo
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26825024 - 07/15/20 11:52 AM (3 years, 8 months ago)

What happened to them? Agar to agar great idea do that for clean mycelium :smile:


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InvisibleRoger Clemency
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Re: Inoculating and incubating agar plates [Re: Kmacmo]
    #26825043 - 07/15/20 11:58 AM (3 years, 8 months ago)

Yeah 2,3,6 look like normal germination plates. You don’t want to use them straight away though you want at least one transfer away from germ. Usually 2 and sometimes more of it’s troublesome.

Those other plates look like a thin mold but hard to tell. You might wanna order some new plates too cuz you can use them up real quick lol.


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OfflineLeglocklarry
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26830114 - 07/18/20 02:58 AM (3 years, 8 months ago)

Ok thanks for the tips. I had four remaining plates already poured and did a transfer (from the second picture I last posted, the zig zag'ed plate). Hopefully these look like buttholes in a week, if not I've already got more plates on the way for more transferring. After that, I'll probably go straight to making LC which I've finally wrapped my head around. Keen to get a magnetic stirrer.


Edited by Leglocklarry (07/18/20 03:11 AM)

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Offlinew00tmycelium
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26830174 - 07/18/20 04:50 AM (3 years, 8 months ago)

Quote:

Leglocklarry said:
Ok thanks for the tips. I had four remaining plates already poured and did a transfer (from the second picture I last posted, the zig zag'ed plate). Hopefully these look like buttholes in a week, if not I've already got more plates on the way for more transferring. After that, I'll probably go straight to making LC which I've finally wrapped my head around. Keen to get a magnetic stirrer.





You are definitely on the right track... looks like a good size transfer and growth outside of those cultures.  I wouldn't worry at all at this point, you can turn those into infinite amounts if you keep them clean.

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OfflineLeglocklarry
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Re: Inoculating and incubating agar plates [Re: w00tmycelium]
    #26837761 - 07/22/20 09:08 AM (3 years, 8 months ago)

How are these looking? I think #1 is ok? Number 4 looks like a contam got in?


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OfflineKmacmo
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Re: Inoculating and incubating agar plates [Re: Leglocklarry]
    #26837985 - 07/22/20 10:51 AM (3 years, 8 months ago)

Looks kinda weak and iffy, does look like mycelium though.
Personally I wouldn't use it, I'd go back a step.
I'd go back to the spore plates take more smaller sized transfers. (from different places)

Maybe it's just the pictures, try get some better pics
Could just use it on spawn and it will be like multispore, giving you a wide variety of genetics to print/clone etc.


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Edited by Kmacmo (07/22/20 11:22 AM)

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