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OfflineCanebrake
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Registered: 05/01/20
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WBS and PE doesn't look right.
    #26806151 - 07/06/20 02:57 AM (3 years, 6 months ago)

I usually use corn but decided to try WBS. I cleaned it well. Heated it up to close to boiling but not quite. Let it set for 24 hours. Let dry until it wasn't leaving any wet spots on tissue Etc.....

I used a PE multispore syringe to inoculate. 6-23 was when I did it and this is the result. The seed looks dry as hell and it is covered with what looks like a white powder. I've shaken it once. Usually with my corn I'm damn near fully colonized. I did 8 WBS jars with different strains and they all are not showing real good growth.

Before anyone goes into how horrible MS syringes are. I am aware. I have about 5 different strains and 40-50 agar dishes now that I'm transfering and working with.

Is it too dry? Can I save this but going ahead and adding it to substrate?

Any Ideas would be appreciated.




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InvisibleGoatrider
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Re: WBS and PE doesn't look right. [Re: Canebrake]
    #26806161 - 07/06/20 03:18 AM (3 years, 6 months ago)

2nd pic there`s a bad spot at the bottom,
guess you`ll get green.

As you said MS is russian roulette.

Don`t spawn this, you won`t have any benefits.

Sit it out and look what`s going on.

You have many agar dishes, so i wouldn`t mind that loss my friend :wink:


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OfflineCanebrake
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Re: WBS and PE doesn't look right. [Re: Goatrider]
    #26807353 - 07/06/20 03:45 PM (3 years, 6 months ago)

Quote:

Goatrider said:
2nd pic there`s a bad spot at the bottom,
guess you`ll get green.

As you said MS is russian roulette.

Don`t spawn this, you won`t have any benefits.

Sit it out and look what`s going on.

You have many agar dishes, so i wouldn`t mind that loss my friend :wink:




I went back and looked at my jars. I think that is just a glare or something. There isnt any green on it anywhere.

Any other thoughts?


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InvisibleGoatrider
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Re: WBS and PE doesn't look right. [Re: Canebrake]
    #26807422 - 07/06/20 04:19 PM (3 years, 6 months ago)

Ok, was reflection then.

I think you got a WBS brand that needs a bit more moisture.
Perhaps it should be a little bit more wet outside,
when you load the jars.

You shook the jars a few times after cooldown?


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InvisibleRoger Clemency
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Re: WBS and PE doesn't look right. [Re: Goatrider]
    #26807436 - 07/06/20 04:26 PM (3 years, 6 months ago)

Moisture looks fine to me. The seeds seem plump and I can see a few that are burst. It just looks like a jar that was shaken and isn't recovering because of contamination of some kind.


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Invisiblesandman420
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Re: WBS and PE doesn't look right. [Re: Roger Clemency]
    #26807463 - 07/06/20 04:43 PM (3 years, 6 months ago)

I'm pretty sure this is some kind of powdery mildew or yeast. I doubt he has shaken a jar shot with spores on 6/23 yet or to have that much "growth"


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InvisibleGoatrider
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Re: WBS and PE doesn't look right. [Re: sandman420]
    #26808103 - 07/06/20 11:39 PM (3 years, 6 months ago)

To OP:

You didn`t shake up right after inoculation, did you?
Not good to do with syringe.

As you mentioned you shook up once, how did it look before shaking?


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OfflineCanebrake
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Re: WBS and PE doesn't look right. [Re: Goatrider]
    #26808368 - 07/07/20 06:43 AM (3 years, 6 months ago)

Quote:

Goatrider said:
To OP:

You didn`t shake up right after inoculation, did you?
Not good to do with syringe.

As you mentioned you shook up once, how did it look before shaking?




I shoock it when I saw the hint of white. I didn't look correct to me so I shook it to see if it came back. I'd say around day 8. All I did when I injected was roll the seed to get the spores spread through out the jar.


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InvisibleRoger Clemency
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Re: WBS and PE doesn't look right. [Re: Canebrake]
    #26808391 - 07/07/20 07:13 AM (3 years, 6 months ago)

I might butcher the technical terms here but there’s like mono and dikaryotik mycelium. When spores first germinate they make monokaryotik myc and can’t form mushrooms until they hook up with another mono-d to make dikaryotik mycelium. If you shake them too early you will have those thinner gray looking strands all spread out so that they have to travel to find a partner.

I don’t know if that weakens the myc but it’s caused real long colonization times when I shook early. In this case though I think your myc already had some nasty with it and when you shook it the mold got spread around.


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OfflineJustweed
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Re: WBS and PE doesn't look right. [Re: Roger Clemency]
    #26808454 - 07/07/20 08:01 AM (3 years, 6 months ago)

Definitely looks like contam to me. 2 weeks old and next to no myc indicates a problem.


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OfflineCanebrake
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Re: WBS and PE doesn't look right. [Re: Roger Clemency]
    #26808546 - 07/07/20 08:57 AM (3 years, 6 months ago)

Quote:

Roger Clemency said:
I might butcher the technical terms here but there’s like mono and dikaryotik mycelium. When spores first germinate they make monokaryotik myc and can’t form mushrooms until they hook up with another mono-d to make dikaryotik mycelium. If you shake them too early you will have those thinner gray looking strands all spread out so that they have to travel to find a partner.

I don’t know if that weakens the myc but it’s caused real long colonization times when I shook early. In this case though I think your myc already had some nasty with it and when you shook it the mold got spread around.




One thing I noticed about the syringe was that it didn't have spores like every other syringe I've gotten. It had a jelly looking substance. I read the PE were difficult to get spores from so I thought maybe it was normal.

I put some to 3 plates of agar and was only able to get enough to transfer 2 sections. Of those 2 sections only one made a good enough section to transfer. Luckily it made 7 transfers. So its cleaning up well.


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InvisiblePastywhyteMDiscord
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Re: WBS and PE doesn't look right. [Re: Canebrake]
    #26808715 - 07/07/20 10:16 AM (3 years, 6 months ago)

That looks effed. Start over. Sometimes spores germinate in the syringe, it doesn’t mean the syringe isn’t viable. However it’s extremely difficult to get a truly axenic syringe and PE variants are even more difficult. I just cleaned a yeasty APE syringe myself. Agar is the only way if you want any control over the results.


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