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OfflineMishra288
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First timer question
    #26805662 - 07/05/20 05:20 PM (3 years, 6 months ago)

Hey everyone! Hoping for a little feedback here mostly for reassurance. I got some GT spores, sterilized, and injected into a 5-grain bag. Injection was roughly 5ccs spread out. I inoculated over a month ago. Saw my first signs of mycelium nearly three weeks later. A few weeks after, I gave the bag a "break and shake" and saw lots of new growth pretty quickly. Said new growth has slowed to a crawl now but im not near 100% colonized. First pic is june 5th before the shake up, second is july 5th-today. My question here really is do I keep waiting, or do I take what I have and case them now?

https://i.ibb.co/yBXJTsP/IMG-5471.jpg

https://i.ibb.co/1v0MbFp/IMG-6047.png


Edited by Mishra288 (07/05/20 05:23 PM)


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Offlineroarkell
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Re: First timer question [Re: Mishra288]
    #26805680 - 07/05/20 05:34 PM (3 years, 6 months ago)

You dont want to spawn uncolonized grains.  Very good way to contam before first flush. Also, the second pic looks like the water content is way to high... could also be bacterial...


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OfflineMishra288
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Re: First timer question [Re: roarkell]
    #26805691 - 07/05/20 05:44 PM (3 years, 6 months ago)

Maybe move them out to a dryer location to reduce the water? What would you suggest?


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Offlineroarkell
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Re: First timer question [Re: Mishra288]
    #26805720 - 07/05/20 06:06 PM (3 years, 6 months ago)

I'm not as familiar with bags but if you can increase FAE you'd be in better shape... as for the slow colonization, it could be any number of things...


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OfflineMishra288
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Re: First timer question [Re: roarkell]
    #26805791 - 07/05/20 07:03 PM (3 years, 6 months ago)

Well, they're sealed, with a filter patch for air exchange. I moved them to what I hope are drier conditions with better air flow but opening the bag in my apt would definitely invite contams. I'd hate to lose two months of work. Moving forward I may try jars and see if that works out better.


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Offlinemarvins
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Re: First timer question [Re: Mishra288]
    #26805867 - 07/05/20 07:45 PM (3 years, 6 months ago)

If you read a bit in the forum you will notice that to inoculate grains with spores is not the best option and will probably fail.

You have to clean the spores in agar before inoculation.


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Offlinemarvins
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Re: First timer question [Re: marvins]
    #26805873 - 07/05/20 07:47 PM (3 years, 6 months ago)

Btw agar is not that difficult and really fun if you like mushrooms.


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