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KuroNeko
Mycelium Tycoon


Registered: 05/19/20
Posts: 150
Loc: North Korea
Last seen: 7 months, 3 days
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Please help me with my first agar plates. Have some issues and need advice.
#26804880 - 07/05/20 09:30 AM (3 years, 6 months ago) |
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Hello again. Those are my first plates. I will describe each of them individually. Can you please help me to know what's going on with each of them and tell me which plates/sections I should transfer to new plates?
Plate A: Tissue taken from inside of mushroom, this was clean from the beginning.
 
Plate B: Tissue taken from inside of mushroom, this was clean but new growth appeared after couple of days, scary at first but I think it's satellite mycelium from when tissue sample tumbled a bit inside of plate before I forced it to stick in the center.
 
Plate C: Same as plate B.
 
Plate D: Tissue taken from mycelium, this had some bacterial spots around tissue sample but it looks like mycelium outrun bacteria and is eating it. Spots became smaller with time if looking from the bottom.
 
Plate E: Same as plate D
 
To me plate A and E look best but I'm concerned about bacterial spots that were present before they were covered with mycelium. I should start practicing cleaning anyway. So please tell me which are best to take transfers from. I have 10 plates left.
Also, can I let any of those plates that were clean grow and inoculate jars or is second clean transfer mandatory?
-------------------- English not my native language, please excuse grammar.
 
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redhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
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Re: Please help me with my first agar plates. Have some issues and need advice. [Re: KuroNeko]
#26804893 - 07/05/20 09:43 AM (3 years, 6 months ago) |
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Dude all your plates look good, well done. So in terms of where you choose to transfer: you want to take it from the edge that has come furthest along from the center. Preferably spots that look clean and organized with rhizo growth.
For example plate E you can take from 10, 1 and 7 o'clock. Plate D from 2 and maybe 6-8 o'clock. But these plates look so good you could virtually take form any edge that is stretching outwards naturally. So my suggestions are just that: suggestions.
Plate B I agree that that may be satelite myc but we growers dont like it when things are growing where they are not supposed to. Plate A is a really nice plate.
So I think for you its more or less choose what you want. But make sure to take very small transfers from the very edge for our T1s, and then transfer to T2 after just a few days of growth so you dont give any possible contams the chance to grow with the myc Well done OP!
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Please help me with my first agar plates. Have some issues and need advice. [Re: redhandmat]
#26804913 - 07/05/20 09:54 AM (3 years, 6 months ago) |
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Congratulations.
First timers plates rarely look like these.
Very good job!
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KuroNeko
Mycelium Tycoon


Registered: 05/19/20
Posts: 150
Loc: North Korea
Last seen: 7 months, 3 days
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Re: Please help me with my first agar plates. Have some issues and need advice. [Re: redhandmat]
#26804921 - 07/05/20 10:00 AM (3 years, 6 months ago) |
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Thank you guys, those are 5 out of 10. Remaining 5 were obviously bad with bacteria and mold with no hope of clean transfers. Are we sure that B and C is satellite mycelium and not some other species of wispy mold?
I think I will leave B and C because they don't look organised and aggressive. Will watch what will happen when two fronts of myc meet. I'm not interested in huge yields. I do it for fun, learning and challenge. I'm going to take nicest bits from E and D to learn cleaning from contaminated plates and also some from A.
If T1 will be next transfer do we call plates such as mine T0?
Would you ever put plate A straight to grain if it grows clean to the edges or you never inoculate with T0?
I made many jars with different rye prep methods some time ago to see if they will contaminate. They are fine, can't wait to inoculate something for first time.
-------------------- English not my native language, please excuse grammar.
 
Edited by KuroNeko (07/05/20 10:11 AM)
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Re: Please help me with my first agar plates. Have some issues and need advice. [Re: KuroNeko]
#26804954 - 07/05/20 10:16 AM (3 years, 6 months ago) |
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It could be the lighting, but C looks like it has mold at the top of the culture. I like A best too. Also if you ever see mold or bacteria and then later you don't, it's still in there. That's why it's recommended to transfer early from the cleanest edge. Looking good though, nice work.
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redhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
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Re: Please help me with my first agar plates. Have some issues and need advice. [Re: LadysKnight]
#26804968 - 07/05/20 10:26 AM (3 years, 6 months ago) |
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Quote:
KuroNeko said: If T1 will be next transfer do we call plates such as mine T0?
Would you ever put plate A straight to grain if it grows clean to the edges or you never inoculate with T0?
We call them germination plates because we let the spores germinate on them. I dont know if that changes for clones, such as for your tissue plates. T stands for transfer, so first transfer is T1, second T2, etc...
I have put germination plates that looked good to grains, but first I took transfers from them so that I would have a backup. The reason was that I really wanted to have fruits fast and was willing to take the risk of losing the jar before the T2s could grow out. But I really wouldn't recommend it.
Also, even if your plate is entirely clean, I don't know if that a good idea when the plate has tissue on it though? I have never done it so not sure if the mushroom piece will rot inside the grains jar or not.
Quote:
LadysKnight said: It could be the lighting, but C looks like it has mold at the top of the culture. I like A best too. Also if you ever see mold or bacteria and then later you don't, it's still in there. That's why it's recommended to transfer early from the cleanest edge. Looking good though, nice work.
Also what LK said, if you see contams in there at a stage and they later seem to have miraculously disappeared, they haven't. So its best to have a couple of clean transfers between that plate and the one you put to grains. Dont wait too long between transfers either You win both in terms of time and in terms of getting ahead of contams.
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