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OfflineYoyoma
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No-pour agar PC question.
    #26803954 - 07/04/20 04:54 PM (3 years, 7 months ago)

So I'm planning to do a version of no-pour agar with the below container, a half pint mason jar. I'll mix the agar, pour it in the jars then PC.



My question is, do I need modified lids? Do I need to loosen them or can I leave as is?
Then can I just take the cooled jars straight to the SAB to inoculate?


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OfflineGan
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26803961 - 07/04/20 05:01 PM (3 years, 7 months ago)

Yeah I'd recommend modifying the lids. With plastic lids, I'm sure you could go the unmodified route and loosen the lid a bit. But I've always been iffy about the loose lids route


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OfflineYoyoma
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Re: No-pour agar PC question. [Re: Gan]
    #26803980 - 07/04/20 05:17 PM (3 years, 7 months ago)

I can't just modify the metal ones? I already bought extra lol.


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InvisibleThe Fresh PrintsS
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804073 - 07/04/20 06:13 PM (3 years, 7 months ago)

Just a GE hole with micropore tape will work


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OfflineYoyoma
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Re: No-pour agar PC question. [Re: The Fresh Prints]
    #26804133 - 07/04/20 06:58 PM (3 years, 7 months ago)

Should I replace the lid after inoculation with no hole or do I just keep the same lid?


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Invisiblepablokabute
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804141 - 07/04/20 07:04 PM (3 years, 7 months ago)

Just drill or punch a small hole, a small one, maybe 1/8-1/4 of an inch to be covered with half inch micropore. I do a couple layers 2 or maybe 3 layers. Yes, that thing would work for agar, in fact, I personally prefer glass jars over pp5 plastics.

You dont replace the lid, its the same lid throughout its life. You dont even have to replace the micropore for your succeeding batches with the same jar.

Good luck with your agar venture. Its really fun and enjoyable.


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OfflineEntheoGod
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Re: No-pour agar PC question. [Re: pablokabute]
    #26804168 - 07/04/20 07:32 PM (3 years, 7 months ago)

Good luck with the agar. Im currently getting my technique with it down as well. :smile: its gonna feel good when I finally put something healthy to grain. I wish you the best of luck and look forward to updates if you decide to post em! I use the pp5 pastyplates tek to make my agar and agar plates. I think the glass would feel much better imo though.


Edited by EntheoGod (07/04/20 07:32 PM)


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OfflineYoyoma
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Re: No-pour agar PC question. [Re: EntheoGod]
    #26804191 - 07/04/20 07:48 PM (3 years, 7 months ago)

Thanks!
Yeah I'm using these small glass jars for agar then I plan to transfer them to Qt. pp5 plastic grain bottles for the remainder of the grow using the Bottle Tek


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InvisibleTheDoobskerS
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Re: No-pour agar PC question. [Re: EntheoGod]
    #26804196 - 07/04/20 07:53 PM (3 years, 7 months ago)

Personally, I also like glass.

I'm pretty new to mycology, but have some cell biology lab experience. Science research labs are so wasteful it's insane. Some of it is most definitely justified, but not all of it. Every time I throw away a pp5 I have a vision of landfills flash in my mind.
I read a post from RR weighing the difference in waste-fullness of plastic dishes compared to all the added waste of cleaning and sterilizing glass ones. He states that glass dishes are more harmful to the environment. Very valid points that he made, but it's really hard to compare the two.

I use borosilicate glass petri dishes which are more difficult to PC than half pint jars. On the contrary, with mason jars you lose the important factor of being able to view your dishes from directly above your growth which will be a lot more annoying than it may sound.


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OfflineYoyoma
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Re: No-pour agar PC question. [Re: TheDoobsker]
    #26804217 - 07/04/20 08:13 PM (3 years, 7 months ago)

How often do you have to throw away PP5 plastics? Are they not reusable?
How do you clean your glass ones?


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Invisiblepablokabute
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804235 - 07/04/20 08:24 PM (3 years, 7 months ago)

You color your agar and that visibility issue disappears.. coupled with a high wattage lighting above your SAB, its almost non-issue now. Whats the explanation behind short glass jars and single use petri? Ive used pp5 pastyplates for many years and each time, problems pop up here and there. When I used glass jars, I finally got comftable with agar.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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InvisibleTheDoobskerS
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804308 - 07/04/20 09:10 PM (3 years, 7 months ago)

Quote:

Yoyoma said:
How often do you have to throw away PP5 plastics? Are they not reusable?
How do you clean your glass ones?




Oh dude, every single day, multiple times a day. Petri dishes are used for everything. Whether you're studying/growing bacteria, fungi, or even mammalian cells, you use petri dishes. I've never even heard of an actual lab using glass ones, it's always plastic.
Reason being, they come pre-sterilized and 100% clean from the package, so you don't have to spend any extra time worrying about also making those sterile on top of all of your other worries of sterilization.

Theoretically, they are re-usable multiple times, but you'd have to sterilize them. They're pretty cheap, so it's not worth the effort. Also, they'll melt in a PC, so that poses issues too.

I sterilize my glass ones in the oven. Wrap them in foil and bake for 2 hours at 320F; seems to work just fine. I do this around the same time that I PC my agar media bottle. If it works out correctly, the dishes are cooled at the same time that my agar reaches 120F and then I pour them in my SAB.

I should add though, this method almost always results in at least a few specs of junk in your agar. It's sterile, so it won't cause any issues, but it is slightly annoying. It's nice to just open your bag of pp5 petris and be certain that you have nothing but plastic in your hand to pour into.


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InvisibleTheDoobskerS
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Re: No-pour agar PC question. [Re: TheDoobsker]
    #26804311 - 07/04/20 09:12 PM (3 years, 7 months ago)

Quote:

TheDoobsker said:
Oh dude, every single day, multiple times a day.




Okay, this was a bit of an exaggeration when talking about an individual in a lab. You won't always have agar work to do every day. But if you pick out any bio lab at any university, they will have multiple petri dishes in the garbage every day.


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OfflineGan
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804391 - 07/04/20 10:48 PM (3 years, 7 months ago)

Quote:

Yoyoma said:
I can't just modify the metal ones? I already bought extra lol.




Yeah of course you can modify the metal ones. I was just saying IF you wanted to go the unmodified route, plastic lids are best


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OfflineYoyoma
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Re: No-pour agar PC question. [Re: TheDoobsker]
    #26804571 - 07/05/20 03:01 AM (3 years, 7 months ago)

Those petris are not PP5 then if they melt. That's what PP5 means, that it will be heat resistant.


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InvisibleSunny Skies
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26804592 - 07/05/20 04:05 AM (3 years, 7 months ago)

the 5 after PP is the recycle code


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InvisibleTheDoobskerS
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Re: No-pour agar PC question. [Re: Yoyoma]
    #26805140 - 07/05/20 12:08 PM (3 years, 7 months ago)

Quote:

Yoyoma said:
Those petris are not PP5 then if they melt. That's what PP5 means, that it will be heat resistant.




Hmm maybe I'm wrong then. I've never tried to PC/autoclave them, but I thought I had seen a post on here showing the aftermath of someone who did. I guess they weren't pp5


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InvisibleSunny Skies
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Re: No-pour agar PC question. [Re: TheDoobsker]
    #26805162 - 07/05/20 12:23 PM (3 years, 7 months ago)

I use 8oz deli dishes for my no pour.cheap and cheerful and the same size as a 90mm petri. I drill a hole in the side cover with tape, wrap them loosely in foil then PC for 40min.


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