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OfflineHoopersCaps
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Registered: 07/04/20
Posts: 22
Last seen: 3 years, 5 months
First Timer Spawn-Bulk Questions
    #26803982 - 07/04/20 05:19 PM (3 years, 7 months ago)

Hello! I’m new to all this, and I just got everything in place for grain spawn. Im using the north spore 3 pound grain spawn. For the first bag, I sterilized everything with rubbing alcohol, the bag, injection port, the syringe. I inserted about 3cc and I moved it all around and then put away. Is it going to be a problem since I shakes up that bag after I injected the syringe? I know people usually wait Until it’s about 30% colonized.  I then plan on putting that into a 66qt monotub. Is there any recommendations for beginners as far as substrate and a casing layer?  Is there any major benefits from using dung/manure with a coir casing? Also, what’s the best way to find the right ration for colonized grain spawn-fruiting chamber/substrate?


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: First Timer Spawn-Bulk Questions [Re: HoopersCaps]
    #26804011 - 07/04/20 05:35 PM (3 years, 7 months ago)

Inoculating and shaking is normal.
Did you use a spore syringe or a liquid culture syringe?
For substrate I recommend just using coir or maybe coir and verm.


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Offlinemetaphoric
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Registered: 05/18/15
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Re: First Timer Spawn-Bulk Questions [Re: Strainsfordaze]
    #26804043 - 07/04/20 05:53 PM (3 years, 7 months ago)

Normally you don't shake right away when you're using spores. You need spores close together so they can touch each other and germinate.

You shake right away when you're using LC, because LC has already been germinated.

In your case though I'm sure you'll be fine because you used so many CCs of spores. Next time you only need to use .5 CCs and don't shake.

Also, flame sterilize your needle instead of alcohol.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26804066 - 07/04/20 06:09 PM (3 years, 7 months ago)

Good catch metaphoric.


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OfflineHoopersCaps
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Registered: 07/04/20
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26804067 - 07/04/20 06:10 PM (3 years, 7 months ago)

Okay. It was a Spore syringe, and I did use a flame right before I injected into the grain spawn. I used Rubbing alcohol on everything including the syringe then used a flame on the needle. You recommend that little of the syringe for a 3 pound bag? I used about 3cc on the other two spawn bags. So right now I have one 3 pound with about 2.5 mixed up, and the other two 3 pound bags have about 3-3.5cc but not mixed. Is having to much effect the spawn? Will it be open to more contamination because of the overdoing and moisture when it colonizes?


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: Strainsfordaze]
    #26804082 - 07/04/20 06:19 PM (3 years, 7 months ago)

It was a spore syringe. (Golden Teacher). Okay I’m now thinking i might have jumped to far in for my first time.  What’s the best way to determine the ratio for colonized spawn-substrate?  Is there a good way to decide how big of a tub and how much substrate based on the spawn?  I have three 3pound bags right now.  Thank you for the response!


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OfflineSpirit-Crusher
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Registered: 04/24/19
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Re: First Timer Spawn-Bulk Questions [Re: HoopersCaps]
    #26804089 - 07/04/20 06:25 PM (3 years, 7 months ago)

Get ready for your spawn bags to most likely contaminate. Taking spores from a syringe directly to a spawn bag is a recipe for disaster. The problem probably wont be the bag because it seems like you bought pre-sterilized ones, the problem is syringes are rarely ever sterile.

Hate to be the bearer of bad news, but if you are going to use straight spores to grain, you'll have better luck using jars.


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: Spirit-Crusher]
    #26804098 - 07/04/20 06:33 PM (3 years, 7 months ago)

Even with sterilizing the syringe entirely with ISO and flame before injecting? If it’s coming from a reputable source?(Spore Depot)What would be the main difference between doing the north spore grain spawn bags, vs jars?


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Offlinemetaphoric
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Re: First Timer Spawn-Bulk Questions [Re: HoopersCaps]
    #26804223 - 07/04/20 08:18 PM (3 years, 7 months ago)

.5 CCs is equally effective as 3 CCs and you can make 6x more bags.

Spirit Crusher is right but also exaggerating a bit. I use MS syringes straight to grain jars and I have about 5% failure rate. I do think you're better off with jars because they'll finish quicker and shaking is easier but I think you've got decent a chance at success rn.

Keep us updated!


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26804240 - 07/04/20 08:28 PM (3 years, 7 months ago)

I see. Does adding more CC’s just speed up the colonization? Seems like I wasted a decent amount. I’m going to try Jars next time, but for now I’m just gonna wait and see how it turns out. Thinking about mixing it around 15% instead of 30 to see if that helps or makes a different. Right now they are just in a box wrapped in plastic to help seal it off better. Thank you for the advice, I will update this thread as soon as I start to see some results!


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OfflineSpirit-Crusher
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Re: First Timer Spawn-Bulk Questions [Re: HoopersCaps]
    #26804472 - 07/05/20 12:24 AM (3 years, 7 months ago)

The problem is the contamination is riding along in the syringe. Think about how there made. Prints are used to scrape spores into sterilized water. Very rarely are the spore prints taken from fruits that were grown in 100% sterile environment.

You'll have better luck with jars because they colonize quicker and the mycelium has a better chance of beating out the bacteria or mold that rode along with the spores. I tried it a bunch. Trust me, yes you'll get a few that will allow you to flush before they go bad, but I about 3/4 of the bags I made contaminated before they were even fully colonized. I know how much time goes into prepping spawn bags and pressure cooking them, those are success rates that just don't sit well with me. But you do you boo. You can read and take others advice so much, but ultimately you'll figure it out after you've wasted countless hours and money failing with them. I wish you the best of luck though. You might get lucky this time, but next time maybe not. I like consistency and predictability.


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26807518 - 07/06/20 05:14 PM (3 years, 7 months ago)

http://


This is one of the bags out of three. It’s been 2 days. I’m pretty sure it’s moisture. Any suggestions?


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Offlinemetaphoric
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Registered: 05/18/15
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Re: First Timer Spawn-Bulk Questions [Re: HoopersCaps]
    #26807544 - 07/06/20 05:23 PM (3 years, 7 months ago)

You're not going to see anything after 2 days. Try to put it away and don't look at it for a week.


Also your grains are over hydrated, see how they're all burst? It shouldn't affect your myc though.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineMrSolo
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Registered: 11/11/11
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26807620 - 07/06/20 06:09 PM (3 years, 7 months ago)

Turn the bag upside down to help with the moisture.


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OfflineMrSolo
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Re: First Timer Spawn-Bulk Questions [Re: MrSolo]
    #26807623 - 07/06/20 06:13 PM (3 years, 7 months ago)

Hi all, I'm having bags (rye berries) stall out at 80-90% growth. Now some are showing that orange/yellow "piss". Is it too late to save these guys? Should I break them up and hope they fully colonize? Let them keep sitting and hope they fully colonize? it's been about 50 days in the closet.


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Offlinemetaphoric
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Re: First Timer Spawn-Bulk Questions [Re: MrSolo]
    #26807643 - 07/06/20 06:28 PM (3 years, 7 months ago)

They're most likely contaminated. Did you go syringe to bag? That's tough, jars are less likely to contam ime.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineMrSolo
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26807655 - 07/06/20 06:33 PM (3 years, 7 months ago)

Yep, syringe to bag. Bags were all sterilized, same with syringes. Sucks...


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26809527 - 07/07/20 05:27 PM (3 years, 7 months ago)

Yeah. I flipped that one bag over. I wasn’t expecting to see anything. I just happened to look and saw that. Will continue to update!! Thanks for the advise.


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OfflineHoopersCaps
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Re: First Timer Spawn-Bulk Questions [Re: MrSolo]
    #26810898 - 07/08/20 12:02 PM (3 years, 7 months ago)






This is after flipping upside down.


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OfflineHoopersCaps
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Last seen: 3 years, 5 months
Re: First Timer Spawn-Bulk Questions [Re: metaphoric]
    #26900300 - 08/26/20 10:54 AM (3 years, 5 months ago)

Update! Have a 32qt tub going into FAE as of yesterday. Let me know what y’all think and if this looks healthy or could be cobweb mold. Could be overthinking. If so is it to late? Hydrogen peroxide?







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