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Offlinekgq
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Registered: 05/19/20
Posts: 17
Last seen: 3 years, 4 months
What am I doing wrong?
    #26803610 - 07/04/20 01:40 PM (3 years, 6 months ago)

So I am at my third Tub now and things don't go as planned. I followed Bods Unmodified tek as closely as I could (I just swapped the Oats he used for Rye).

I'm on day 14 now and by now I should see some pins, but I see nothing, just white everywhere.. no knots, no hyphae, no pins, but no visible contamination either I think. I'm ready to give this tub up too, but I am unsure what to do differently next.

I hope you can tell something from these pictures:




My tub has 4 open holes which I taped over, leaving just a slit for FAE.

My inoculant is a LC I made myself with super clean agar slices from an MS Syringe. The LC itself showed clean growth on agar, just a few metabolites, but nothing major.

There is just one thing that is making me anxious: I took a sample from one of these thick white mycelium balls on the side of my tub and put it on agar and it came back bacterial:



Should I be worried? Should I give up on this tub and focus on the next one? But what should I do differently this time?


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Offlinelefty24
Stranger
Registered: 06/02/20
Posts: 72
Last seen: 3 years, 6 months
Re: What am I doing wrong? [Re: kgq]
    #26803619 - 07/04/20 01:49 PM (3 years, 6 months ago)

I have brf to bulk 2:5, 1:3, and 1:4 brf spawn to coir and my tubs are pinning anywhere from 14 days to 30 days. About half the time I pin on or by day 14.

It's def not a lost cause. Be patient.


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,809
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Re: What am I doing wrong? [Re: lefty24]
    #26803626 - 07/04/20 01:53 PM (3 years, 6 months ago)

I see knots forming in there. Pins will be in a few days.


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