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ledemonhacker
Stranger
Registered: 07/04/20
Posts: 10
Last seen: 3 years, 5 months
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question about sterilization process
#26803245 - 07/04/20 09:58 AM (3 years, 6 months ago) |
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Hi Guys,My first post here, Not sure if it's in the right place or not, So I got an autoclave that reaches 30 PSI,it looks like an electric all american autoclave, this is my first experience and i have been throwing a lot of jars.
I'm doubting it's maybe because I use the autoclave at 30PSI for 25 mins. Can that be the issue?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: question about sterilization process [Re: ledemonhacker]
#26803258 - 07/04/20 10:06 AM (3 years, 6 months ago) |
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Damn, 30psi. I would shoot for 15-19 psi for 90 mins for jars, and 180-210 mins for bags.
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
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Re: question about sterilization process [Re: ledemonhacker]
#26803266 - 07/04/20 10:09 AM (3 years, 6 months ago) |
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That is badass, congrats. However 25 minutes sounds way too short, even with the higher pressure
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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ledemonhacker
Stranger
Registered: 07/04/20
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Re: question about sterilization process [Re: Gastronomicus]
#26803274 - 07/04/20 10:13 AM (3 years, 6 months ago) |
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So leaving it at 30 psi for 40 mins(Jars) and 60 mins(Bags) would be ok?
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 14 hours
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Re: question about sterilization process [Re: ledemonhacker]
#26803277 - 07/04/20 10:14 AM (3 years, 6 months ago) |
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surprised the backup valve didn't purge
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: question about sterilization process [Re: AtmozFear]
#26803288 - 07/04/20 10:20 AM (3 years, 6 months ago) |
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Bags will melt, and your blowout valve most likely will blowout. Why do you want to run it that high? 15-19 is totally fine. Sometimes mine will wander up to 21-22 but I quicker lower it back down.
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ledemonhacker
Stranger
Registered: 07/04/20
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Last seen: 3 years, 5 months
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Re: question about sterilization process [Re: Strainsfordaze]
#26803300 - 07/04/20 10:23 AM (3 years, 6 months ago) |
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Surprisingly I can't control the heat, It came this way, So it's fixed for 30 PSI lol
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ledemonhacker
Stranger
Registered: 07/04/20
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Last seen: 3 years, 5 months
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Re: question about sterilization process [Re: ledemonhacker]
#26804754 - 07/05/20 07:30 AM (3 years, 6 months ago) |
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So i tried it yesterday on 30 PSI for 55 mins and no jars broke or anything, Yet the grains went a bit burned or something. I inoculated them just for testing. I'm guessing i'll buy a traditional pressure cooker.
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redhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
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Re: question about sterilization process [Re: ledemonhacker]
#26804805 - 07/05/20 08:18 AM (3 years, 6 months ago) |
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Quote:
SiliconGhost said:
Quote:
eve.kalika said: This autoautoclave actually operates at closer to 30 psi, so a shorter time can be used.
Ultimately, there's only one way to find out. I can leave a blank control to ensure it's not contaminated from inadequate sterilizing.
How long after sterilizing can I keep a grain jar?
15 is the minimum. I run 45 minutes cycles at 30 PSI and 121˚C. I've not had one contam on it's on after autoclaved. My contams are usually the result of me cracking the lids to inoculate. I now work in front of a flow hood in one of the other in vitro labs down the hall.
From this thread https://www.shroomery.org/forums/showflat.php/Number/20811011#20811011
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ledemonhacker
Stranger
Registered: 07/04/20
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Re: question about sterilization process [Re: redhandmat]
#26804994 - 07/05/20 10:43 AM (3 years, 6 months ago) |
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That's really good to know! I will try it on 30PSI for 45 mins and see what happens.
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