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Offlineledemonhacker
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Registered: 07/04/20
Posts: 10
Last seen: 3 years, 5 months
question about sterilization process
    #26803245 - 07/04/20 09:58 AM (3 years, 6 months ago)

Hi Guys,My first post here, Not sure if it's in the right place or not,
So I got an autoclave that reaches 30 PSI,it looks like an electric all american autoclave, this is my first experience and i have been throwing a lot of jars.

I'm doubting it's maybe because I use the autoclave at 30PSI for 25 mins. Can that be the issue?




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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: question about sterilization process [Re: ledemonhacker]
    #26803258 - 07/04/20 10:06 AM (3 years, 6 months ago)

Damn, 30psi. I would shoot for 15-19 psi for 90 mins for jars, and 180-210 mins for bags.


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OfflineGastronomicus
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Re: question about sterilization process [Re: ledemonhacker]
    #26803266 - 07/04/20 10:09 AM (3 years, 6 months ago)

That is badass, congrats. However 25 minutes sounds way too short, even with the higher pressure


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Make my Funk the P Funk, I wants to get Funked up

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Offlineledemonhacker
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Re: question about sterilization process [Re: Gastronomicus]
    #26803274 - 07/04/20 10:13 AM (3 years, 6 months ago)

So leaving it at 30 psi for 40 mins(Jars) and 60 mins(Bags) would be ok?


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OfflineAtmozFear
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Re: question about sterilization process [Re: ledemonhacker]
    #26803277 - 07/04/20 10:14 AM (3 years, 6 months ago)

surprised the backup valve didn't purge  :undecided:


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: question about sterilization process [Re: AtmozFear]
    #26803288 - 07/04/20 10:20 AM (3 years, 6 months ago)

Bags will melt, and your blowout valve most likely will blowout.
Why do you want to run it that high?
15-19 is totally fine.
Sometimes mine will wander up to 21-22 but I quicker lower it back down.


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Offlineledemonhacker
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Re: question about sterilization process [Re: Strainsfordaze]
    #26803300 - 07/04/20 10:23 AM (3 years, 6 months ago)

Surprisingly I can't control the heat, It came this way, So it's fixed for 30 PSI lol


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Offlineledemonhacker
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Re: question about sterilization process [Re: ledemonhacker]
    #26804754 - 07/05/20 07:30 AM (3 years, 6 months ago)

So i tried it yesterday on 30 PSI for 55 mins and no jars broke or anything, Yet the grains went a bit burned or something. I inoculated them just for testing.
I'm guessing i'll buy a traditional pressure cooker.


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: question about sterilization process [Re: ledemonhacker]
    #26804805 - 07/05/20 08:18 AM (3 years, 6 months ago)

Quote:

SiliconGhost said:
Quote:

eve.kalika said:
This autoautoclave actually operates at closer to 30 psi, so a shorter time can be used.

Ultimately, there's only one way to find out. I can leave a blank control to ensure it's not contaminated from inadequate sterilizing.

How long after sterilizing can I keep a grain jar?




15 is the minimum. I run 45 minutes cycles at 30 PSI and 121˚C. I've not had one contam on it's on after autoclaved. My contams are usually the result of me cracking the lids to inoculate. I now work in front of a flow hood in one of the other in vitro labs down the hall.




From this thread https://www.shroomery.org/forums/showflat.php/Number/20811011#20811011


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Offlineledemonhacker
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Re: question about sterilization process [Re: redhandmat]
    #26804994 - 07/05/20 10:43 AM (3 years, 6 months ago)

That's really good to know!
I will try it on 30PSI for 45 mins and see what happens.


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