Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
OfflineYoyoma
Stranger
Registered: 07/24/18
Posts: 34
Last seen: 3 years, 5 months
Is this normal growth??
    #26802565 - 07/04/20 12:44 AM (3 years, 6 months ago)

Help me identify if this is normal growth for these plates so far. I'm a noob doing agar for the first time. I will list what I did and my mistakes and the photos following. Please let me know if anything is the results are my mistakes.

-June 28 innc. (5 days)
-Used 4 pre-poured agar plates
-Used 2 different spore syringes (B+ plates 1/2, Golden T plates 3/4)
-SAB
-Did not shake the syringes prior
-Flame sterilized needle only before plate 1 and 3
-Did not cool the needle in agar before squirting
-Squirted multiple drops because I was nervous
-Did NOT go back and streak with loop

edit: ignore the black markings (reflection)


PLATE 1 seems like nothing is going on




PLATE 2 seems like Mycelium growth




PLATE 3 not sure what's going on here




PLATE 4 also not sure




Edited by Yoyoma (07/04/20 12:58 AM)


Extras: Filter Print Post Top
OfflineGastronomicus
3-0-G
Male User Gallery


Registered: 03/31/05
Posts: 9,727
Last seen: 2 hours, 55 minutes
Re: Is this normal growth?? [Re: Yoyoma]
    #26802613 - 07/04/20 01:12 AM (3 years, 6 months ago)

Plate 1 is contaminated unless those specks are sediment in the agar, plate 3 and 4 are totally fucked.

Plate 2 looks like myc but it's also super wispy and disorganized and might be mold. Take a transfer to a clean plate and wait and see.


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


Extras: Filter Print Post Top
OfflineYoyoma
Stranger
Registered: 07/24/18
Posts: 34
Last seen: 3 years, 5 months
Re: Is this normal growth?? [Re: Gastronomicus]
    #26802616 - 07/04/20 01:16 AM (3 years, 6 months ago)

pretty sure it's specs in the agar on plate 1

should I go ahead and transfer plate 2 already? Any small edge??

what on earth is going on with 3 and 4??


Extras: Filter Print Post Top
OfflineSoupyGeorge
Hazy Daze since First Grade
Male


Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
Re: Is this normal growth?? [Re: Yoyoma]
    #26802620 - 07/04/20 01:19 AM (3 years, 6 months ago)

Spores ball up in the syringes. Shaking is a must to break the spore clusters up. It's possible your first plate didn't get any spores. Wait a few days to make sure though.

I did not cool my needle either and my plates colonized just fine. Your second plate indeed looks like Mycelium.

The two last plates are both contaminated with bacteria, that's the puddle like substance you see. Possibly from the syringe itself considering they are the only two with that contamination.

It is best to only use one single drop from a very well shaken syringe and either streak it with an inoculation loop or let it roll in a straight line from on side of the dish to the other and set it down not disturbing it for a while. Both methods have worked for me.


--------------------
Mellow heads will prevail.

You look like everyone else because the T.V. tells you to.
I look like everyone else so that i don't stand out to the man.
We are not the same.


Extras: Filter Print Post Top
OfflineYoyoma
Stranger
Registered: 07/24/18
Posts: 34
Last seen: 3 years, 5 months
Re: Is this normal growth?? [Re: SoupyGeorge]
    #26802655 - 07/04/20 01:43 AM (3 years, 6 months ago)

Thank you for the info!

Yes, I was nervous so I did forget a few steps. I did not realize I wouldn't be able to see the drops in the agar. I also only had 4 agar plates. I won't be able to make more either until a week from now, so any more inoculations or transfer of plate 2 will have to wait.

Disappointed and a little surprised about plate 3/4 as I thought my technique had improved from the previous plates lol. But as you stated it must have been from the syringe. Is this just a matter of getting a good single drop spread?


Extras: Filter Print Post Top
Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Is this normal growth?? [Re: Yoyoma]
    #26802668 - 07/04/20 01:53 AM (3 years, 6 months ago)

From the plate that looks like myc, you should take transfers (T1) as soon as you can. very small from the best looking area.


Extras: Filter Print Post Top
OfflineYoyoma
Stranger
Registered: 07/24/18
Posts: 34
Last seen: 3 years, 5 months
Re: Is this normal growth?? [Re: redhandmat]
    #26802684 - 07/04/20 02:02 AM (3 years, 6 months ago)

Would I ever take it from the middle or only from the edges?


Extras: Filter Print Post Top
Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Is this normal growth?? [Re: Yoyoma]
    #26802696 - 07/04/20 02:20 AM (3 years, 6 months ago)

Quote:

Yoyoma said:
Would I ever take it from the middle or only from the edges?




The edge is where you want to take your transfers.

In your future plates try to get clear agar with no sediments so that you are truly in the know of what is what. Check teks on how to get it like that. What I personally do is I have clear LME in powder form, add it with the agar (right amount of both ofc), add the right amount of water (I like adding it boiling hot but that's not necessary probably). Then I use a mixer or manually mix it really well while its boiling. The results are clear agar plates that you can see through (like glass).


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Re: Normal growths? neverwhere 1,027 4 05/30/00 04:10 AM
by Vorpal
* Growth slow...Any ideas what to do? Pics!!!! savageharvester 2,459 10 05/27/02 09:56 AM
by indicaz
* mycelium growth (time line)? Jsonfield 3,314 6 11/22/03 12:16 AM
by Cloud9
* White mold/contamination or perfectly normal? PIC omegatron 2,728 3 03/03/02 03:07 PM
by Seuss
* Wild Bird Seed... no growth after 6 days.. HidingInPlainSight 2,085 16 04/07/03 03:48 PM
by athena
* No growth detected! Help! Dionysus 1,304 11 06/12/03 08:30 PM
by Dionysus
* malt/dextrose sediment in LC with stir plate davesj1 2,454 15 07/03/06 10:07 PM
by Omnicracker
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
129 topic views. 23 members, 197 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.