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Offline3some
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Registered: 04/20/20
Posts: 278
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Spore print to LME agar failure
    #26802487 - 07/03/20 11:52 PM (3 years, 7 months ago)

Fresh spore to agar


Dried spores to agar


Absolute failure. Would antibiotic be the answer? Or some other selective media?


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Ask me about growing yeast, mold and bacteria. I'm very experienced. :tongue2:

The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners


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InvisibleTheDoobskerS
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Registered: 05/22/20
Posts: 799
Re: Spore print to LME agar failure [Re: 3some]
    #26802521 - 07/04/20 12:12 AM (3 years, 7 months ago)

Antibiotic or selective media is a concept I'm familiar with but haven't read anything about it in relation to mycology.

I'm sure people use it, but my point is, I don't think it's where you should be looking.

What's the story of these spores? It seems like maybe you've just had contams be introduced in many different steps along the path from mushroom cap to agar growth.


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Offline3some
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Re: Spore print to LME agar failure [Re: TheDoobsker]
    #26802531 - 07/04/20 12:18 AM (3 years, 7 months ago)

The fresh ones are wild subs fresh off the forest floor. So I expected contaminations. Just thought that some myc will appear as well but they didn't.

The dried one are galindoi sent by a friend. Same thing. Expected contaminations AND some mycelium growth as well.

Any advice on how to make it work?


--------------------
Ask me about growing yeast, mold and bacteria. I'm very experienced. :tongue2:

The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners


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InvisibleTheDoobskerS
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Registered: 05/22/20
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Re: Spore print to LME agar failure [Re: 3some]
    #26802554 - 07/04/20 12:36 AM (3 years, 7 months ago)

I have no experience with growing from wild mushrooms, but I think you'd be better off cloning instead of trying to grow from the spores. Reason being, you clone from the inside where there are much less contams.

For the dried print, you could try streaking it onto the plate if you aren't already doing that? That's more of a chance that myc will show up somewhere that you can transfer from.


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Offline3some
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Re: Spore print to LME agar failure [Re: TheDoobsker]
    #26802576 - 07/04/20 12:50 AM (3 years, 7 months ago)

Quote:

TheDoobsker said:
I have no experience with growing from wild mushrooms, but I think you'd be better off cloning instead of trying to grow from the spores. Reason being, you clone from the inside where there are much less contams.

For the dried print, you could try streaking it onto the plate if you aren't already doing that? That's more of a chance that myc will show up somewhere that you can transfer from.




I'll try to do that. Thank you.

What I'm doing at the moment is dipping the swab into LME LC and hope that it will grow. And when I see anything that looks like myc, I'll fish it out and put it back onto agar.


--------------------
Ask me about growing yeast, mold and bacteria. I'm very experienced. :tongue2:

The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners


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OfflineForevergreen
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Registered: 06/23/20
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Last seen: 3 years, 5 months
Re: Spore print to LME agar failure [Re: 3some]
    #26802603 - 07/04/20 01:09 AM (3 years, 7 months ago)

Quote:

3some said:
The fresh ones are wild subs fresh off the forest floor. So I expected contaminations. Just thought that some myc will appear as well but they didn't.

The dried one are galindoi sent by a friend. Same thing. Expected contaminations AND some mycelium growth as well.

Any advice on how to make it work?




Im also a newbie, havent grown anything yet. From what ive read, you definitely want to add that antibacterial agent to your agar when messing with wild mushrooms. Its usually for cloning, but im sure wild spores are the same concept, you got some crazy contams in those spores and nothing anyone can do about it, they're wild.


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InvisibleTheDoobskerS
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Re: Spore print to LME agar failure [Re: 3some]
    #26802612 - 07/04/20 01:12 AM (3 years, 7 months ago)

Aaah yeah that sounds pretty dirty. Think of it this way: your prints most definitely have contaminants all over them. Most prints do. If you streak them onto agar, you might have a few streaked sections where there were little contams, and the mycelium outcompeted and grew well. This is where you would transfer from to clean it up. But if you take that same dirty print and dip it into a LC solution, you just introduced all of those contams into the same media bottle and mixed it all together.
Maybe this is a legit method I just haven't come across, but it just sounds unnecessarily risky/dirty.

Also, maybe this is just something I'm not familiar with too, but what's up with that swab paper? Spore are most commonly printed onto foil.


These prints you have may just be a lost cause, but if you live in an area where you can find some wild shrooms jsut go grab more and clone some :wink::thumbup:


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Offline3some
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Re: Spore print to LME agar failure [Re: TheDoobsker]
    #26802624 - 07/04/20 01:23 AM (3 years, 7 months ago)

Quote:

TheDoobsker said:
Aaah yeah that sounds pretty dirty. Think of it this way: your prints most definitely have contaminants all over them. Most prints do. If you streak them onto agar, you might have a few streaked sections where there were little contams, and the mycelium outcompeted and grew well. This is where you would transfer from to clean it up. But if you take that same dirty print and dip it into a LC solution, you just introduced all of those contams into the same media bottle and mixed it all together.
Maybe this is a legit method I just haven't come across, but it just sounds unnecessarily risky/dirty.

Also, maybe this is just something I'm not familiar with too, but what's up with that swab paper? Spore are most commonly printed onto foil.


These prints you have may just be a lost cause, but if you live in an area where you can find some wild shrooms jsut go grab more and clone some :wink::thumbup:




I did make cardboard tacos from the stem butts. The genius that discovered this cardboard tek deserves a Nobel Peace Prize. Works like a charm.

This is just to practice germinating from spores while I have them. Learning opportunity. I even transfered some myc from the cardboard onto agar. Looks like failure so far.

I printed my spores on baking paper. Easier to see color.


--------------------
Ask me about growing yeast, mold and bacteria. I'm very experienced. :tongue2:

The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Spore print to LME agar failure [Re: 3some]
    #26802664 - 07/04/20 01:50 AM (3 years, 7 months ago)

Quote:

3some said:
What I'm doing at the moment is dipping the swab into LME LC and hope that it will grow. And when I see anything that looks like myc, I'll fish it out and put it back onto agar.




Yeah dude, feels like you are killing your chances of getting clean myc because of this. LC is already difficult to get clean and keep clean, let alone when you knowingly dip contams in it and give them an environment to grow before taking them to agar. Antibiotics can help but they dont do anything against molds, only bacteria. And it looks like you have mold too.

What you could try is to do a bunch of plates, and remember where you streaked. Maybe even use a swab and put the whole cut off swab on a plate and see what happens. Just try a bunch of things, work in a SAB/FH and keep things as tidy and clean as you can to decrease chances of contamination. Im sure one of these plates will give you something to transfer and work with.


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