Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures, Substrate Bags   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Cultivation Supplies, Injection Grain Bag

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Spore syringe ==> LME Agar Recipe?
    #26801918 - 07/03/20 05:43 PM (3 years, 7 months ago)

I've used the same recipe of LME agar to inoculate with spore syringes 4 times, and I've only seen one germination point on 1 plate out of 10-20.

500ml water
20g agar
15g LME

I'm assuming this is too hard. Would using like 18g agar soften it up enough? Should I increase weight of LME also?


Extras: Filter Print Post Top
InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801924 - 07/03/20 05:47 PM (3 years, 7 months ago)

Your recipe is for 1000ml I would cut that in half.

500ml water
10g agar
7-10grams malt


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801930 - 07/03/20 05:51 PM (3 years, 7 months ago)

Shit sorry I looked at the wrong part of my notes. Yeah I've been doing 10g agar and 8g LME per 500ml media bottle.

If I drop it to 9g agar and increase LME to 9g or 10g, maybe that's good for 20 plates to noc w/ spores?


Extras: Filter Print Post Top
InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801956 - 07/03/20 06:03 PM (3 years, 7 months ago)

Sure, 1g + or - won’t be a deal breaker.
How long have you waiting for your spores to germinate?
It can take 2-3 weeks if on the slower side.


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801967 - 07/03/20 06:10 PM (3 years, 7 months ago)

I've done one drop in the center, several drops, then used an inoculation loop, and each time I get a tiny germination after like 2 weeks, and then nothing else after like a month or more. Not even contamination growth.

I'll tweak it to 9g agar 10g LME for inoculation plates, and pour others with 10g agar 10g LME for transfers. We'll see...


Extras: Filter Print Post Top
InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801989 - 07/03/20 06:19 PM (3 years, 7 months ago)

Seems like the spores are the problem.


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801998 - 07/03/20 06:24 PM (3 years, 7 months ago)

Maybe. But they're from a legit source, and I've tried with 3 different syringes of 3 different varieties. I'll try the Costa Rica syringe I havnt used yet for 10 plates and see what happens


Extras: Filter Print Post Top
InvisibleShenmue
Dark Lord of the Sith 
Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26802004 - 07/03/20 06:26 PM (3 years, 7 months ago)

I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.


Extras: Filter Print Post Top
InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802030 - 07/03/20 06:39 PM (3 years, 7 months ago)

How long are you pressure cooking your agar?


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26802044 - 07/03/20 06:46 PM (3 years, 7 months ago)

20min @ 15psi


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26802072 - 07/03/20 06:56 PM (3 years, 7 months ago)

Also been taking syringes out of the fridge to warm up and shaking the shit out of them before each squirt


Extras: Filter Print Post Top
InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26802073 - 07/03/20 06:57 PM (3 years, 7 months ago)

Seems like your doing everything by the book.
Let us know how it goes on these next ones.


Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802121 - 07/03/20 07:17 PM (3 years, 7 months ago)

Quote:

Shenmue said:
I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.




Learn something new everyday. Pre-made agar on etsy nice. Sometimes you just want to pay for the convenience (although agar is easy enough for most). Anyone know of a Canadian source for these pre-made ones? Amazon has plain ones, but i love the color on the etsy ones. Shipping is a killer from US.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
Re: Spore syringe ==> LME Agar Recipe? [Re: maxmush]
    #26802216 - 07/03/20 08:17 PM (3 years, 7 months ago)

Are you streaking your plates?


--------------------



Extras: Filter Print Post Top
OfflineCrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 7 months, 17 days
Re: Spore syringe ==> LME Agar Recipe? [Re: Gan]
    #26802225 - 07/03/20 08:24 PM (3 years, 7 months ago)

Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.


--------------------
:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:



Extras: Filter Print Post Top
OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest]
    #26802234 - 07/03/20 08:29 PM (3 years, 7 months ago)

Quote:

CrashTest said:
Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.




Ooooof that's steep. Why not try no-pour agar?


--------------------



Extras: Filter Print Post Top
InvisibleShenmue
Dark Lord of the Sith 
Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest] * 1
    #26802291 - 07/03/20 08:57 PM (3 years, 7 months ago)

Quote:

CrashTest said:
Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.




Dude they're only 35$ for 20 on etsy with free shipping. You got ripped off.


Extras: Filter Print Post Top
InvisibleShenmue
Dark Lord of the Sith 
Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802293 - 07/03/20 08:59 PM (3 years, 7 months ago)



Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802325 - 07/03/20 09:28 PM (3 years, 7 months ago)

Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.


Extras: Filter Print Post Top
InvisibleShenmue
Dark Lord of the Sith 
Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs] * 1
    #26802413 - 07/03/20 10:19 PM (3 years, 7 months ago)

Quote:

AlpineHighs said:
Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.




I don't have to. I have a good job so I have the money to blow on mycology slaves lol. I'll watch a movie and masturbate while a college kid does it for me :yesnod: ...


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Liquid Cultures, Substrate Bags   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Cultivation Supplies, Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Agar recipe work well for germinating these five spores? KingCobWeb 9,957 8 02/24/03 11:48 PM
by Anno
* Simple Agar recipe please? ATWAR 44,525 18 12/14/20 01:25 PM
by bigfootscreepyuncl
* spore life and inoculating agar JonnyOnTheSpot 1,133 1 01/31/02 05:45 PM
by djveiox
* Interesting agar recipes from the Radical Mycology book
( 1 2 all )
krock 12,625 23 08/05/23 01:21 PM
by balisonggrow
* agar recipe arabmobster 2,881 4 04/06/03 08:08 PM
by arabmobster
* spore syring is in trouble? mulan 14,150 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,685 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,957 2 06/01/00 08:17 PM
by 3Mshroom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,567 topic views. 38 members, 165 guests and 56 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 14 queries.