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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Second round of isolating on agar, thoughts?
    #26802054 - 07/03/20 06:52 PM (3 years, 6 months ago)

Hey all,

I'm attaching a few plates from my second round of transfers, trying to isolate a strong performing APE. It's my first time with APE and I'm still learning how to maximize my performance with agar, so I'm looking for any suggestions on how to keep progressing and getting better.

I had about 10 plates this round, but these 5 seemed to be the best performing. Not sure if I should let them grow more, do some transfers, or what. One thing I have noticed is that the mycelium gets "fuzzy".. is this something I should worry about, or something I can dial into to reduce?

Any advice is super appreciated, thanks!

Note*: Assume 12 o'clock on the plates are the plate number.



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InvisibleGoatrider
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Registered: 04/08/20
Posts: 4,396
Loc: Germany Flag
Re: Second round of isolating on agar, thoughts? [Re: Dr. Sporesly]
    #26802171 - 07/03/20 07:49 PM (3 years, 6 months ago)

Looking fine!

A true isolate is much work and lots of transfers,
and no warranty for fruiting.

You may transfer once more, for there are different sectors on each plate,
but since it's from multispore, i'd inoculate now.

Make a good clone then and start over.

There are some with rhizo growth, some tomentose, and also combined.
It doesn't matter, since it's healthy.

That's the size i prefer to use for inoculation, perhaps 2 days more.
                                  :cookiemonster:


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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Second round of isolating on agar, thoughts? [Re: Goatrider]
    #26803005 - 07/04/20 08:07 AM (3 years, 6 months ago)

Quote:

Goatrider said:
Looking fine!

A true isolate is much work and lots of transfers,
and no warranty for fruiting.

You may transfer once more, for there are different sectors on each plate,
but since it's from multispore, i'd inoculate now.

Make a good clone then and start over.

There are some with rhizo growth, some tomentose, and also combined.
It doesn't matter, since it's healthy.

That's the size i prefer to use for inoculation, perhaps 2 days more.
                                  :cookiemonster:





Many thanks!

If I were to continue transferring one or two plates (and quadrants) for isolation with the goal to grow a high performing variety, are there any that you would suggest?

It's taken me a while to get this far with the plates and would like to continue perfecting them but also want to try my first APE grow as well.


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InvisibleGoatrider
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Registered: 04/08/20
Posts: 4,396
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Re: Second round of isolating on agar, thoughts? [Re: Dr. Sporesly]
    #26803026 - 07/04/20 08:22 AM (3 years, 6 months ago)

Is that still from multispore, as you say variety?
If so, then it doesn't matter.
You want a strain, so you have to grow out.
With multispore, you just do transfers til you get clean myc.
Then put to grains and grow out.
You'll have strains then, and take a good one to clone.
Put it on agar, and transfer strong sectors.


--------------------


Edited by Goatrider (07/04/20 08:27 AM)


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Second round of isolating on agar, thoughts? [Re: Dr. Sporesly]
    #26803059 - 07/04/20 08:40 AM (3 years, 6 months ago)

Quote:

Dr. Sporesly said:

If I were to continue transferring one or two plates (and quadrants) for isolation with the goal to grow a high performing variety, are there any that you would suggest?

It's taken me a while to get this far with the plates and would like to continue perfecting them but also want to try my first APE grow as well.




Depends how you define high performing. Are you talking potency or fast growing, or something else?

Usually for most growers, we transfer from the fastest growing part of the myc on the agar. On agar we can only see fast growth, health and cleanness, as well as tomentose or rhisomorphic growth. So we try to select the best parts to transfer, but we know nothing about potency, fruiting capability and potential speed on other mediums than agar.

Then once we fruit it we usually try to clone mushrooms from clusters that are large and hopefully potent, so that the next gen we grow will have more clusters and bigger fruits etc. Other growers are interested of other characteristics so they clone those instead.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Second round of isolating on agar, thoughts? [Re: redhandmat]
    #26803060 - 07/04/20 08:40 AM (3 years, 6 months ago)

Also, what is the recipe of your agar?


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InvisibleGoatrider
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Posts: 4,396
Loc: Germany Flag
Re: Second round of isolating on agar, thoughts? [Re: redhandmat]
    #26803080 - 07/04/20 08:46 AM (3 years, 6 months ago)

I just remember...
Wasn't there a thread with guys telling APE's do better by multispore?


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OfflineDr. Sporesly
Papa Smurf
Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Second round of isolating on agar, thoughts? [Re: redhandmat]
    #26808265 - 07/07/20 04:24 AM (3 years, 6 months ago)

Quote:

Goatrider said:
Is that still from multispore, as you say variety?
If so, then it doesn't matter.
You want a strain, so you have to grow out.
With multispore, you just do transfers til you get clean myc.
Then put to grains and grow out.
You'll have strains then, and take a good one to clone.
Put it on agar, and transfer strong sectors.




Correct they’re multispore from a reputable vendor suggested by Shroomery. I took the syringe to agar and after a ton of bad plates got one.


Quote:

redhandmat said:
Also, what is the recipe of your agar?




Been using MEA plates, so: 8-10g dried malt extract and 8-10g agar agar with 500ml water.

Going to do a final round of transfers today and I’ll just grab the fastest growing myc I can see. Thanks everyone!


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