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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Spore syringe ==> LME Agar Recipe?
#26801918 - 07/03/20 05:43 PM (3 years, 7 months ago) |
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I've used the same recipe of LME agar to inoculate with spore syringes 4 times, and I've only seen one germination point on 1 plate out of 10-20.
500ml water 20g agar 15g LME
I'm assuming this is too hard. Would using like 18g agar soften it up enough? Should I increase weight of LME also?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26801924 - 07/03/20 05:47 PM (3 years, 7 months ago) |
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Your recipe is for 1000ml I would cut that in half.
500ml water 10g agar 7-10grams malt
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Shit sorry I looked at the wrong part of my notes. Yeah I've been doing 10g agar and 8g LME per 500ml media bottle.
If I drop it to 9g agar and increase LME to 9g or 10g, maybe that's good for 20 plates to noc w/ spores?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26801956 - 07/03/20 06:03 PM (3 years, 7 months ago) |
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Sure, 1g + or - won’t be a deal breaker. How long have you waiting for your spores to germinate? It can take 2-3 weeks if on the slower side.
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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I've done one drop in the center, several drops, then used an inoculation loop, and each time I get a tiny germination after like 2 weeks, and then nothing else after like a month or more. Not even contamination growth.
I'll tweak it to 9g agar 10g LME for inoculation plates, and pour others with 10g agar 10g LME for transfers. We'll see...
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26801989 - 07/03/20 06:19 PM (3 years, 7 months ago) |
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Seems like the spores are the problem.
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Maybe. But they're from a legit source, and I've tried with 3 different syringes of 3 different varieties. I'll try the Costa Rica syringe I havnt used yet for 10 plates and see what happens
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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802030 - 07/03/20 06:39 PM (3 years, 7 months ago) |
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How long are you pressure cooking your agar?
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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20min @ 15psi
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26802072 - 07/03/20 06:56 PM (3 years, 7 months ago) |
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Also been taking syringes out of the fridge to warm up and shaking the shit out of them before each squirt
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26802073 - 07/03/20 06:57 PM (3 years, 7 months ago) |
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Seems like your doing everything by the book. Let us know how it goes on these next ones.
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802121 - 07/03/20 07:17 PM (3 years, 7 months ago) |
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Quote:
Shenmue said: I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.
Learn something new everyday. Pre-made agar on etsy nice. Sometimes you just want to pay for the convenience (although agar is easy enough for most). Anyone know of a Canadian source for these pre-made ones? Amazon has plain ones, but i love the color on the etsy ones. Shipping is a killer from US.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: maxmush]
#26802216 - 07/03/20 08:17 PM (3 years, 7 months ago) |
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Are you streaking your plates?
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 7 months, 17 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: Gan]
#26802225 - 07/03/20 08:24 PM (3 years, 7 months ago) |
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Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.
--------------------
this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest]
#26802234 - 07/03/20 08:29 PM (3 years, 7 months ago) |
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Quote:
CrashTest said: Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.
Ooooof that's steep. Why not try no-pour agar?
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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest] 1
#26802291 - 07/03/20 08:57 PM (3 years, 7 months ago) |
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Quote:
CrashTest said: Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.
Dude they're only 35$ for 20 on etsy with free shipping. You got ripped off.
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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802293 - 07/03/20 08:59 PM (3 years, 7 months ago) |
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802325 - 07/03/20 09:28 PM (3 years, 7 months ago) |
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Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.
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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs] 1
#26802413 - 07/03/20 10:19 PM (3 years, 7 months ago) |
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Quote:
AlpineHighs said: Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.
I don't have to. I have a good job so I have the money to blow on mycology slaves lol. I'll watch a movie and masturbate while a college kid does it for me ...
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