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OfflineAlpineHighs
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Registered: 04/22/20
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Spore syringe ==> LME Agar Recipe?
    #26801918 - 07/03/20 05:43 PM (3 years, 6 months ago)

I've used the same recipe of LME agar to inoculate with spore syringes 4 times, and I've only seen one germination point on 1 plate out of 10-20.

500ml water
20g agar
15g LME

I'm assuming this is too hard. Would using like 18g agar soften it up enough? Should I increase weight of LME also?


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801924 - 07/03/20 05:47 PM (3 years, 6 months ago)

Your recipe is for 1000ml I would cut that in half.

500ml water
10g agar
7-10grams malt


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OfflineAlpineHighs
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Registered: 04/22/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801930 - 07/03/20 05:51 PM (3 years, 6 months ago)

Shit sorry I looked at the wrong part of my notes. Yeah I've been doing 10g agar and 8g LME per 500ml media bottle.

If I drop it to 9g agar and increase LME to 9g or 10g, maybe that's good for 20 plates to noc w/ spores?


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801956 - 07/03/20 06:03 PM (3 years, 6 months ago)

Sure, 1g + or - won’t be a deal breaker.
How long have you waiting for your spores to germinate?
It can take 2-3 weeks if on the slower side.


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801967 - 07/03/20 06:10 PM (3 years, 6 months ago)

I've done one drop in the center, several drops, then used an inoculation loop, and each time I get a tiny germination after like 2 weeks, and then nothing else after like a month or more. Not even contamination growth.

I'll tweak it to 9g agar 10g LME for inoculation plates, and pour others with 10g agar 10g LME for transfers. We'll see...


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26801989 - 07/03/20 06:19 PM (3 years, 6 months ago)

Seems like the spores are the problem.


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26801998 - 07/03/20 06:24 PM (3 years, 6 months ago)

Maybe. But they're from a legit source, and I've tried with 3 different syringes of 3 different varieties. I'll try the Costa Rica syringe I havnt used yet for 10 plates and see what happens


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InvisibleShenmue
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Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26802004 - 07/03/20 06:26 PM (3 years, 6 months ago)

I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802030 - 07/03/20 06:39 PM (3 years, 6 months ago)

How long are you pressure cooking your agar?


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26802044 - 07/03/20 06:46 PM (3 years, 6 months ago)

20min @ 15psi


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26802072 - 07/03/20 06:56 PM (3 years, 6 months ago)

Also been taking syringes out of the fridge to warm up and shaking the shit out of them before each squirt


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26802073 - 07/03/20 06:57 PM (3 years, 6 months ago)

Seems like your doing everything by the book.
Let us know how it goes on these next ones.


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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802121 - 07/03/20 07:17 PM (3 years, 6 months ago)

Quote:

Shenmue said:
I've been buying agar Dishes Pre-poured on etsy . It's more expensive but it's so nice to have everything done from the very beginning. If I was constantly growing mushrooms all year long I would definitely do it myself because it saves money but spending $35 bucks a year is nothing to me.




Learn something new everyday. Pre-made agar on etsy nice. Sometimes you just want to pay for the convenience (although agar is easy enough for most). Anyone know of a Canadian source for these pre-made ones? Amazon has plain ones, but i love the color on the etsy ones. Shipping is a killer from US.


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OfflineGan
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Re: Spore syringe ==> LME Agar Recipe? [Re: maxmush]
    #26802216 - 07/03/20 08:17 PM (3 years, 6 months ago)

Are you streaking your plates?


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OfflineCrashTest
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Registered: 06/21/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Gan]
    #26802225 - 07/03/20 08:24 PM (3 years, 6 months ago)

Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.


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:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:



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OfflineGan
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Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest]
    #26802234 - 07/03/20 08:29 PM (3 years, 6 months ago)

Quote:

CrashTest said:
Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.




Ooooof that's steep. Why not try no-pour agar?


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InvisibleShenmue
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Re: Spore syringe ==> LME Agar Recipe? [Re: CrashTest] * 1
    #26802291 - 07/03/20 08:57 PM (3 years, 6 months ago)

Quote:

CrashTest said:
Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.




Dude they're only 35$ for 20 on etsy with free shipping. You got ripped off.


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InvisibleShenmue
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Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802293 - 07/03/20 08:59 PM (3 years, 6 months ago)



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OfflineAlpineHighs
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Registered: 04/22/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802325 - 07/03/20 09:28 PM (3 years, 6 months ago)

Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.


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InvisibleShenmue
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Registered: 12/21/18
Posts: 2,514
Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs] * 1
    #26802413 - 07/03/20 10:19 PM (3 years, 6 months ago)

Quote:

AlpineHighs said:
Invest in agar supplies. I got everything needed on ebay for like $120, including a case of 500 petri dishes.




I don't have to. I have a good job so I have the money to blow on mycology slaves lol. I'll watch a movie and masturbate while a college kid does it for me :yesnod: ...


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OfflineAlpineHighs
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Registered: 04/22/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802417 - 07/03/20 10:25 PM (3 years, 6 months ago)

Haha. Lucky man


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OfflineCrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 29 days
Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802721 - 07/04/20 02:47 AM (3 years, 6 months ago)

Quote:

Shenmue said:
Quote:

CrashTest said:
Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.




Dude they're only 35$ for 20 on etsy with free shipping. You got ripped off.




First time looking for them and only one supplier popped up. I’m just gonna cancel that order and buy these lol


--------------------
:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:



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OfflineCrashTest
Stay Learning


Registered: 06/21/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
    #26802728 - 07/04/20 02:53 AM (3 years, 6 months ago)



--------------------
:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:



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OfflineAlpineHighs
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Registered: 04/22/20
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Re: Spore syringe ==> LME Agar Recipe? [Re: Strainsfordaze]
    #26839214 - 07/22/20 08:24 PM (3 years, 6 months ago)

Well, looks like another failed attempt at MSS to agar. I inoculated 10 plates with a Costa Rica syringe on 7/3.
Only saw 2 tiny growths after ~10 days of what looked like mycelium, and transferred it to 4 new plates on 7/16.

Here's the status today...



Is that even mycelium? Is it worth continuing to transfer, or would I just be transferring contams?


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OfflineGan
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26839289 - 07/22/20 08:55 PM (3 years, 6 months ago)

It's questionable. What's the strength of your nutes in your agar? Also, do you have pics of your previous germination plates you took those transfers from?


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InvisiblemushboyMDiscord
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26839306 - 07/22/20 08:59 PM (3 years, 6 months ago)

Quote:

AlpineHighs said:
Is that even mycelium?




not cube thats for sure. i think thats toast howd your germ plate look?


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: Gan]
    #26839308 - 07/22/20 09:00 PM (3 years, 6 months ago)

10g agar / 9g LME / 500ml H2O

Don't have pics of the 2 originals, but the two growths were weak, about the size of a nickle


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InvisiblemushboyMDiscord
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26839309 - 07/22/20 09:01 PM (3 years, 6 months ago)

bummer try again
:depressing:


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OfflineAlpineHighs
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Re: Spore syringe ==> LME Agar Recipe? [Re: mushboy]
    #26839311 - 07/22/20 09:03 PM (3 years, 6 months ago)

Dammit. Thanks


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OfflineGan
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
    #26839435 - 07/22/20 10:19 PM (3 years, 6 months ago)

Just a tip that helped me out when I was starting. Keep your germ plate (or any plates you transferred from) for a few days, if you have the room. When I was new, a lot of times I thought I was transferring clean myc. But I'd look back at the donor plate and it would become obvious what was truly clean myc I grabbed and what was a kinda similar looking mold.

Definitely not necessary. Especially if you dont have the room. But it helped me for the first couple months to learn to spot healthy myc and contams. It was especially useful for building an eye for those pesky contams that are hidden within the myc.


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