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Offlinemysticalshroom975
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Zero noticable growth. Do I need to start over?
    #26801867 - 07/03/20 05:21 PM (3 years, 6 months ago)

I inoculated some mycology bags with properly hydrated millet using an spore syringes and an injection port.  I put in pretty much a whole 10 mL syringe into each bag and they were thoroughly mixed.  This was five days ago.  And for that period of time, I had it at a constant 82F.  On day 2 or 3, the bags seemed to be a little dry in the inside because the grains were falling freely when I picked them up to gently inspect for growth.  At this time, the humidity was 50%.  I put in some wetted vermiculite in the cooler where they're incubating, which quickly raised the humidity to 80%.  It should be noted that I used a 10 PSI pressure cooker, cooked two bags at 10 PSI for 3 hours, the other two at 10 PSI for 4 hours.  Each bag used a different syringe, and sourced from two different vendors.  Currently, there is zero noticable contamination and no noticable growth.  Any ideas?

Edit 1: They're cubes: transkei, cambodian, mazapatec, and cuban.
Edit 2: The bags smell like playdough, with varying degrees of strength. 


Edited by mysticalshroom975 (07/03/20 07:11 PM)


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OfflineMH5109
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26801878 - 07/03/20 05:27 PM (3 years, 6 months ago)

10ml per bag seems like alot of liquid to add to already hydrated grain. 5 days isn't that long for spores, and you can more than likely take them out of the cooler, pictures would be helpful to see the moisture in the bag. Also I'm probably not going to be the last person to say spores to grain is typically not a good idea, you would be better off using a lc or agar to clean up spores before they go to grain.


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Edited by MH5109 (07/03/20 05:30 PM)


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OfflineRoshambastic21
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26801886 - 07/03/20 05:30 PM (3 years, 6 months ago)

Spores, especially in a MS syringe, take time to germinate, and then spread. Patience is key. I like to squirt a little along the wall just so I don't go mad waiting to find out if it germinated lol

Also, don't worry too much about temp and humidity in your area, if you can sit comfortably naked in the same room as your grow, you should be good :thumbup::heart:


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InvisibleWall.E
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Re: Zero noticable growth. Do I need to start over? [Re: MH5109]
    #26801889 - 07/03/20 05:31 PM (3 years, 6 months ago)

Are they cubes? If so I'd say 82 might be too hot and delaying the growth, but I'm far from an expert. Maybe someone else with more experience could chime in


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InvisibleStrainsfordaze


Registered: 05/10/18
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26801948 - 07/03/20 06:00 PM (3 years, 6 months ago)

Spores to grains are risky because of contamination that may be present. Spores can sometimes take 2 weeks before growth appears.
Bags/jars shouldn’t be in any special cooler to increase humidity.  You don’t want the humidity to be over 60-70% Anything over will increase likeliness of contamination.
Temp 68-75F will work.
Take the bags out of the cooler. Place them somewhere room temp with normal humidity.


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: MH5109]
    #26801993 - 07/03/20 06:20 PM (3 years, 6 months ago)

Quote:

MH5109 said:
10ml per bag seems like alot of liquid to add to already hydrated grain. 5 days isn't that long for spores, and you can more than likely take them out of the cooler, pictures would be helpful to see the moisture in the bag. Also I'm probably not going to be the last person to say spores to grain is typically not a good idea, you would be better off using a lc or agar to clean up spores before they go to grain.




Yeah, I thought that a full syringe would be too much liquid too, but it was actually on accident and they didn't seem to notice the liquid.  I see zero standing water no condensation on the bags. When I tilt a bag, the grains fall freely like sand, so I thought they might be too dry.


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: Roshambastic21]
    #26801995 - 07/03/20 06:23 PM (3 years, 6 months ago)

Quote:

Roshambastic21 said:
Spores, especially in a MS syringe, take time to germinate, and then spread. Patience is key. I like to squirt a little along the wall just so I don't go mad waiting to find out if it germinated lol

Also, don't worry too much about temp and humidity in your area, if you can sit comfortably naked in the same room as your grow, you should be good :thumbup::heart:




It's actually really easy for me to maintain a constant temperature, I have a heating pad in a cooler and am using an inkbird setup.  Do you think 80% humidity is too high?


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: Wall.E]
    #26801999 - 07/03/20 06:24 PM (3 years, 6 months ago)

Quote:

Myc_Hunt said:
Are they cubes? If so I'd say 82 might be too hot and delaying the growth, but I'm far from an expert. Maybe someone else with more experience could chime in




They're cubes: transkei, cambodian, mazapatec, and cuban.


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OfflineMH5109
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26802005 - 07/03/20 06:26 PM (3 years, 6 months ago)

If the grains aren't clumping together and you don't have a bunch of water at the bottom then they are probably pretty good on moisture. 82 is a little warm but its summer time and it won't stop them from growing, they are a tropical species so 80s are ok not necessarily ideal but will more than likely work out. If your spores weren't to dirty.


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: Strainsfordaze]
    #26802012 - 07/03/20 06:29 PM (3 years, 6 months ago)

Quote:

Strainsfordaze said:
Spores to grains are risky because of contamination that may be present. Spores can sometimes take 2 weeks before growth appears.
Bags/jars shouldn’t be in any special cooler to increase humidity.  You don’t want the humidity to be over 60-70% Anything over will increase likeliness of contamination.
Temp 68-75F will work.
Take the bags out of the cooler. Place them somewhere room temp with normal humidity.




Ok, I can take out the vermiculite and that will reduce the humidity to 50%.  What would the ideal temperature be.  I have an inkbird setup so I can set it to anything.  I like the cooler because I've been doing a lot of cleaning and the vacuum stirs thing up quite a bit.  I feel like that wouldn't help reduce contamination.  What are your thoughts?


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: MH5109]
    #26802017 - 07/03/20 06:32 PM (3 years, 6 months ago)

Quote:

MH5109 said:
If the grains aren't clumping together and you don't have a bunch of water at the bottom then they are probably pretty good on moisture. 82 is a little warm but its summer time and it won't stop them from growing, they are a tropical species so 80s are ok not necessarily ideal but will more than likely work out. If your spores weren't to dirty.




What would you say the ideal temperature is for this stage of the process?  I can easily set it to anything and the temperature stays pretty stable, plus or minus 1 degree F.  I got 82 from the gordotek tutorial.


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OfflineMH5109
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26802027 - 07/03/20 06:37 PM (3 years, 6 months ago)

I personally like 70-75f. Im running high 70s to low 80s right now and it seems like the heat is going to continue, now I remember why I don't normally grow in the summer.


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Edited by MH5109 (07/03/20 06:40 PM)


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: MH5109]
    #26802037 - 07/03/20 06:42 PM (3 years, 6 months ago)

Quote:

MH5109 said:
I personally like 70-75f. Im running high 70s to low 80s right now and it seems like the heat is going to continue, now I remember why I don't normally grow in the summer.




Hmm, I always thought that low 80's resulted in faster colinzation times.  I can cool it down to 75.


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OfflineMH5109
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Re: Zero noticable growth. Do I need to start over? [Re: mysticalshroom975]
    #26802045 - 07/03/20 06:47 PM (3 years, 6 months ago)

It will but low 80s will also benefit unfavorable contams as well. Iv always had best luck with 70-75. Your bags will be slightly warmer a couple degrees as well as the mycelium metabolizes the grain it will generate heat, so 82° plus 2-3 degrees is getting up to an unfavorable temp ime.


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Offlinemysticalshroom975
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Re: Zero noticable growth. Do I need to start over? [Re: MH5109]
    #26802110 - 07/03/20 07:13 PM (3 years, 6 months ago)

Update: I just smelled the bags by pushing some air out of the air filter and they smell like playdough, with varying degrees of strength.  Is this a sign of contamination?


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