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OfflineCrashTest
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2cc’s MS Syringe
    #26801541 - 07/03/20 02:10 PM (3 years, 6 months ago)

I got 2cc’s left of my b+ MSS, what’s the best way to utilize it? Should I noc 2 BRF jars or should I try making an LC? I do not have a PC so anything I do will have to use the steam pot.


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OfflineSpirit-Crusher
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801543 - 07/03/20 02:11 PM (3 years, 6 months ago)

Put it in the fridge and wait to you buy a pressure cooker to do anything with it.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: Spirit-Crusher]
    #26801546 - 07/03/20 02:12 PM (3 years, 6 months ago)

Quote:

Spirit-Crusher said:
Put it in the fridge and wait to you buy a pressure cooker to do anything with it.




I could do that but I have more MSS coming, so I was thinking I could do something with it in the mean time


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Offlinepsycho_nauticus
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Re: 2cc’s MS Syringe [Re: CrashTest] * 2
    #26801558 - 07/03/20 02:15 PM (3 years, 6 months ago)

Learn to do agar with it. I'm telling you, it totally changed my cultivating for the best. It's worth the effort and learning.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: psycho_nauticus]
    #26801562 - 07/03/20 02:17 PM (3 years, 6 months ago)

Quote:

psycho_nauticus said:
Learn to do agar with it.




Are Petri dishes pre sterilized? Meaning I could use the steam pot to make my agar solution, pour into Petri dishes and inoc with the MSS utilizing a SAB?


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OfflineEntheoGod
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Re: 2cc’s MS Syringe [Re: psycho_nauticus]
    #26801563 - 07/03/20 02:18 PM (3 years, 6 months ago)

Look up 123 agar and pastyplates. You’ll have so much fun after you learn that and if you’ve done brf cakes before then you’ll be able to make agar easy trust us! 😀


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InvisibleRoger Clemency
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Re: 2cc’s MS Syringe [Re: CrashTest] * 1
    #26801564 - 07/03/20 02:19 PM (3 years, 6 months ago)

I'd just use it on BRF cakes or try going spores to grain just to see if you get lucky. Definitely don't use them for an LC though since it's a recipe for heart break. You need verifiably clean inoculate for an LC, spores are a huge gamble.


Yeah petri's come sterilized and liquid is a lot easier to sterilize than solids so steaming is viable for agar. I dont know how long though but i'm sure someones done it that you can look up.


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OfflineEntheoGod
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801568 - 07/03/20 02:21 PM (3 years, 6 months ago)

Quote:

CrashTest said:
Quote:

psycho_nauticus said:
Learn to do agar with it.




Are Petri dishes pre sterilized? Meaning I could use the steam pot to make my agar solution, pour into Petri dishes and inoc with the MSS utilizing a SAB?




I’m unsure about Petri dishes being sterile I use pastyplates. Yes you do all that but make sure you follow sterile tek obviously. Agar is quickly made so no stressing if you mess up a few times like I have 😅


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: EntheoGod]
    #26801611 - 07/03/20 02:40 PM (3 years, 6 months ago)

Awesome advice guys, thank you.

I don’t have the means to start agar at the moment but will do more research on that.

I got 2 spare jars so I think ima just throw em in the pot for 90 and then noc the two jars with the 2cc’s

Probably my best bet. I have another b+ MSS on the way that I think I’m gonna save for when I start agar


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Edited by CrashTest (07/03/20 02:40 PM)


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OfflineLogicaL ChaosM
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801761 - 07/03/20 04:12 PM (3 years, 6 months ago)

Just use it on some PF jars :shrug: Or use it all on 1 PF jar for faster growth.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: LogicaL Chaos]
    #26801767 - 07/03/20 04:15 PM (3 years, 6 months ago)

Quote:

LogicaL Chaos said:
Just use it on some PF jars :shrug: Or use it all on 1 PF jar for faster growth.




I used to follow the 2:1:1 ratio and this is nearly accurate however still leaves the substrate a bit beyond field capacity, so I always add a bit extra verm so that the substrate seems dry, but clumps up when I press it together. So I think using 2cc on one jar might actually work lol


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OfflineForevergreen
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801773 - 07/03/20 04:20 PM (3 years, 6 months ago)

Keep looking on craigslist for autoclaves and PCs. I got my 25x all american for $300 off craigslist. Im sure you can find one for $100 just a regular PC though.


Edited by Forevergreen (07/03/20 05:56 PM)


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: Forevergreen]
    #26801806 - 07/03/20 04:43 PM (3 years, 6 months ago)

Quote:

Forevergreen said:
Keep looking on craigslist for autoclaves and PCs. I got my 25x all american for $30£ off craigslist. Im sure you can find one for $100 just a regular PC though.




I have roommates so I can use a steam pot with a tight fitting lid and steam for 90 mins fairly inconspicuously. If I had a PC I might have to get an electric burner for “the lab”(my bedroom lol) and run my PC there. Gotta smooth it all over with the lady though lol


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Offlinepsycho_nauticus
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801812 - 07/03/20 04:46 PM (3 years, 6 months ago)

Yes, usually Petri dishes should come sterile already. There is also another option called no-pours. Find out whichever works for you. I still do no pours and never do pours and Petri dishes, and still get the same results. Everything you do with Petris and pouring, you can do with no pours: cloning, isolating, sectoring, etc.

I was very stressed and overwhelmed when learning it, but after trial and error it paid off. The amount of knowledge you attain in the meantime is worth it itself.


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OfflineLogicaL ChaosM
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Re: 2cc’s MS Syringe [Re: CrashTest] * 1
    #26801834 - 07/03/20 05:00 PM (3 years, 6 months ago)

Quote:

CrashTest said:
Quote:

LogicaL Chaos said:
Just use it on some PF jars :shrug: Or use it all on 1 PF jar for faster growth.




I used to follow the 2:1:1 ratio and this is nearly accurate however still leaves the substrate a bit beyond field capacity, so I always add a bit extra verm so that the substrate seems dry, but clumps up when I press it together. So I think using 2cc on one jar might actually work lol




I use 2/3 cup verm, 1/4 cup water, 1/4 cup BRF. Comes out a bit in the drier side which is nice if u wanna add more spore water :thumbup:


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: LogicaL Chaos]
    #26801842 - 07/03/20 05:04 PM (3 years, 6 months ago)

Quote:

LogicaL Chaos said:
Quote:

CrashTest said:
Quote:

LogicaL Chaos said:
Just use it on some PF jars :shrug: Or use it all on 1 PF jar for faster growth.




I used to follow the 2:1:1 ratio and this is nearly accurate however still leaves the substrate a bit beyond field capacity, so I always add a bit extra verm so that the substrate seems dry, but clumps up when I press it together. So I think using 2cc on one jar might actually work lol




I use 2/3 cup verm, 1/4 cup water, 1/4 cup BRF. Comes out a bit in the drier side which is nice if u wanna add more spore water :thumbup:




Yeah I inoculated my first jars in 10 years on 6/21 and you can see that I made them too wet, because I followed the 2:1:1 to a T.

Good progression on colonization though



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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801847 - 07/03/20 05:06 PM (3 years, 6 months ago)

Here are jars that I inoculated on 6/30, these are not recent pics but they’re already starting to colonize.



But as you can see my substrate is a much better consistency


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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801848 - 07/03/20 05:07 PM (3 years, 6 months ago)

you could do alot with 2ccs if you had agar and an instapot.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: panne cyanne]
    #26801852 - 07/03/20 05:09 PM (3 years, 6 months ago)

I do have an instapot, well it’s my roommates but I could certainly steal it for a day and put it in my bedroom lol.

I think instapot only goes to 10-12 psi? How much longer would I have to sterilize my agar solution?


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OfflineLogicaL ChaosM
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26801862 - 07/03/20 05:19 PM (3 years, 6 months ago)

Quote:

CrashTest said:
Here are jars that I inoculated on 6/30, these are not recent pics but they’re already starting to colonize.



But as you can see my substrate is a much better consistency




Yeah, thats what it should look like, sorta like damp sand.


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InvisibleRoger Clemency
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Re: 2cc’s MS Syringe [Re: LogicaL Chaos] * 1
    #26802172 - 07/03/20 07:49 PM (3 years, 6 months ago)

I'd probably do 30-35 min for agar in an instapot.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: Roger Clemency]
    #26802204 - 07/03/20 08:08 PM (3 years, 6 months ago)

Quote:

Roger Clemency said:
I'd probably do 30-35 min for agar in an instapot.





Awesome thanks dude, might be able to get a quicker start on agar than I thought. Right now I have 10 brf jars at various stages(4 each Noc’d 6/21, 6/24, and 6/30). Two of the first four noc’d on 6/21 were contaminated but the other two remaining still look great and maybe 2 weeks before I birth them. I dumped and soaked these 2 jars in 50/50 bleach water for 24h then cleaned with dish soap. Reloaded with more brf and put on the steam pot for 90 mins. Will let these cool overnight and utilize the last 2cc of my MSS tomorrow.  The other 8 jars I noc’d are all looking great so far, excited to hopefully take a few spore prints :smile:


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Offlinelefty24
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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26803640 - 07/04/20 02:00 PM (3 years, 6 months ago)

I use 3ml syringes for 12 jars all the time.

And you can easily stretch 2ml to 10 jars.

1ml per jar is demented.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: lefty24]
    #26803715 - 07/04/20 02:34 PM (3 years, 6 months ago)

Quote:

lefty24 said:
I use 3ml syringes for 12 jars all the time.

And you can easily stretch 2ml to 10 jars.

1ml per jar is demented.




Used it on two jars this morning :cool:


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Re: 2cc’s MS Syringe [Re: CrashTest]
    #26803869 - 07/04/20 04:03 PM (3 years, 6 months ago)


I make these little BRF pucks from the end of a MSS. Once it colonizes I carefully cur it in 4s and noc up quarts.


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OfflineCrashTest
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Re: 2cc’s MS Syringe [Re: Doyledozo]
    #26803956 - 07/04/20 04:55 PM (3 years, 6 months ago)

Quote:

Doyledozo said:

I make these little BRF pucks from the end of a MSS. Once it colonizes I carefully cur it in 4s and noc up quarts.




That’s a great idea!


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OfflineDoyledozo
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Re: 2cc’s MS Syringe [Re: CrashTest] * 1
    #26803967 - 07/04/20 05:08 PM (3 years, 6 months ago)

In that pic is my very first ATL culture.seeing as though I most likely won't fruit them it ensures I can stretch the syringe out. 2 drops will eventually do as many as 4 quarts. I just flame the needle good and don't open it until the BRF is done.
I've also used these for agar then injected sterile LC broth.


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