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OfflineBlue Helix
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Making LC cultivation lids - how and where to get the parts * 2
    #26800865 - 07/03/20 07:02 AM (3 years, 7 months ago)

I've been asked before how to make a filter lid for LCs that do not leak and work perfectly for mushroom growing.  Below is a picture of the lid, and here are the exact components I recommend:



Plastic Widemouth canning lids
https://www.walmart.com/ip/Mainstays-Large-Jar-Lids/21312095

Gaskets for Lids (a must to prevent leaking and superior to the ones in the picture in my opinion)
https://www.amazon.com/gp/product/B07TSQ9DZ1/

Another option for the gasket and lids combined: you can get the gasket and lid together here for less than either alone, but I don't know this brand personally (the others I do): 
https://www.amazon.com/Aozita-Plastic-REGULAR-Mouth-Silicone/dp/B07T1G3MNK/

Vacutainers (BD 366430) with red septums which are about ten times as good as the grey stoppers (note this price is INSANELY low and I just found it today.  I've had friends buy these at $50 for 100-count)
https://www.walmartpetrx.com/p-5289-bd-vacutainer-serum-blood-collection-tubes-10-ml-16-x-100-mm-100-pk.aspx

22um, PTFE (hydrophobic), 32mm syringe filters (at 50 for $20 this is the best deal I've seen.  Also these are very high quality.)
https://www.ebay.com/itm/32mm-PTFE-Syringe-Filter-0-22um-Pore-Size-Non-sterile-10-1000-pieces/153574497916

Price per lid with option 1 above: $2.21
Price per lid with option 2 above: $1.18

To make these, you gotta drill two holes - that's it!  Assuming you are going to use the red septums of the vacutainers as an injection port (which you should use over the separate grey ones which are a lower quality), the two drill bit sizes for the two holes you need to drill are as follows:

Required Drill Bits for Above
Red silicone septum for injection port - 1/2"
Blue hydrophobic filters listed above  - 7/32"


If you are using a hand drill, you better drill a smaller 1/8" or even 7/32" hole to drive the half-inch bit in (or it can jump on you). 

Hope that helps!


Edited by Blue Helix (10/04/20 06:48 PM)


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InvisibleThe Fresh PrintsS
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix] * 1
    #26800921 - 07/03/20 07:33 AM (3 years, 7 months ago)

How much use do you get from the vacutainer caps before theyre compromised?
Are the tubes of any use? 10ml is Probably too small for a slant, eh?


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Offlinelefty24
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Re: Making LC cultivation lids - how and where to get the parts [Re: The Fresh Prints]
    #26800930 - 07/03/20 07:38 AM (3 years, 7 months ago)

Why wouldn't self healing vial stoppers work? So you have to use the vacutainers?


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: The Fresh Prints]
    #26800935 - 07/03/20 07:39 AM (3 years, 7 months ago)

That's the thing.  If you use say a needle of 14-gauge or higher (meaning smaller), those red septums will last a hundred injections if not more.  I use a fat-ass 12-gauge stainless steel needle in them and they still last a long time if I'm careful.  Most people here use 16-gauge needles and they'll last probably 100 to 200 injections if not more with those tiny needles.  The little grey septums last maybe a tenth that.  Given how cheap those vacutainers are (I used to pay twice that price on eBay) from Walmart Pets is now, I wouldn't even bother with the grey stoppers at all.  If you don't want the tubes, just toss them and keep the septums.

PS - I shit you not that I found those red septums on sale on eBay for "mushroom use" for $12 each.  I'm not kidding.


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: lefty24]
    #26800940 - 07/03/20 07:42 AM (3 years, 7 months ago)

Quote:

lefty24 said:
Why wouldn't self healing vial stoppers work? So you have to use the vacutainers?




No, you don't have to use the test tube part of the vacutainers if you don't want.  I happen to store my LCs in the refrigerator in them, but you can throw them away.  The real treasure on those is that red septum which I guess you call a "self-healing vial stopper" if you want.  Go find those for 15 cents each now, though.  You won't find them for that.  I saw them on eBay for $12 EACH a few hours ago even.  The vacutainers are a great deal if you use the test tube part or not.


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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26801008 - 07/03/20 08:13 AM (3 years, 7 months ago)

Yeah I stop trusting the grey SHIPs after their second PC cycle. I'll go ahead and snag a pack of those. Thanks for the heads-up!


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: The Fresh Prints]
    #26801382 - 07/03/20 12:32 PM (3 years, 7 months ago)

Another option for the gasket and lids combined: you can get the gasket and lid together here for less than either alone, but I don't know this brand personally (the others I do): 
https://www.amazon.com/Aozita-Plastic-REGULAR-Mouth-Silicone/dp/B07T1G3MNK/


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26805313 - 07/05/20 01:38 PM (3 years, 7 months ago)

One other comment I should have added above: sometimes the syringe filter discs do clog after repeated use.  I don't know exactly how or why, but it does happen.  Before I put anything in the pressure cooker, I test the filter disc by blowing air with my lips through it.  Air should pass through with some resistance.  If there is no resistance or if it's totally clogged, just replace the filter disc with a new one.


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Invisiblesandman420
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26805461 - 07/05/20 03:04 PM (3 years, 7 months ago)

You like those gaskets with the tall inner rim that stick to the jar like in your first pic? I tried them and found they can come half loose when you take the lid off to drop agar in. The normal clear silicone gaskets without that flange stick to the inside of the lid and stay out of the way a lot better IMO.

edit: I might be thinking of a different brand of gasket that has that extra inner rim like in your first picture that stick to to the jar rim, I guess the county line ones dont have that. Whatever ones have that are a bit of a pain.


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Edited by sandman420 (07/05/20 03:31 PM)


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: sandman420]
    #26805599 - 07/05/20 04:31 PM (3 years, 7 months ago)

Quote:

sandman420 said:
You like those gaskets with the tall inner rim that stick to the jar like in your first pic? I tried them and found they can come half loose when you take the lid off to drop agar in. The normal clear silicone gaskets without that flange stick to the inside of the lid and stay out of the way a lot better IMO.

edit: I might be thinking of a different brand of gasket that has that extra inner rim like in your first picture that stick to to the jar rim, I guess the county line ones dont have that. Whatever ones have that are a bit of a pain.





Sorry, I had the wrong link, and I've corrected it (thank you for pointing this out).  These are the gaskets I use:

https://www.amazon.com/gp/product/B01LWX1BCC/

This type I have not noticed falling in.  Maybe those others would.


Edited by Blue Helix (07/05/20 04:40 PM)


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InvisibleL0rdk4in
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26821980 - 07/13/20 09:55 PM (3 years, 7 months ago)

The amazon page for the lids say not suitable for pressure cooking. Is that true?


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: L0rdk4in]
    #26837090 - 07/21/20 09:01 PM (3 years, 6 months ago)

Quote:

L0rdk4in said:
The amazon page for the lids say not suitable for pressure cooking. Is that true?




I'm actually not sure.  I use the white Mainstays lids like those at Walmart.  They do not warp in the pressure cooker:

https://www.walmart.com/ip/Mainstays-Large-Jar-Lids/21312095

I'll change the link.


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InvisibleL0rdk4in
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26839436 - 07/22/20 10:20 PM (3 years, 6 months ago)

I just got my order of the amazon linked ones in. I will try them this weekend and let you know. If not Ill grab those.


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InvisibleKernale
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26839502 - 07/22/20 11:02 PM (3 years, 6 months ago)

Im not sure if these are exactly the same ports, but they are for the same purpose.

https://www.sciplus.com/lg-orange-stopper-50288-p

20 for $2.50

No tubes, if that matters to you, but it's about half the price.


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InvisiblePastywhyteMDiscord
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Re: Making LC cultivation lids - how and where to get the parts [Re: Kernale]
    #26839601 - 07/23/20 12:03 AM (3 years, 6 months ago)

Nice post. I like a good LC lid, often they are useful for a few things. I love LC too but that’s a different story.


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: Kernale]
    #26839634 - 07/23/20 12:30 AM (3 years, 6 months ago)

Quote:

Kernale said:
Im not sure if these are exactly the same ports, but they are for the same purpose.

https://www.sciplus.com/lg-orange-stopper-50288-p

20 for $2.50

No tubes, if that matters to you, but it's about half the price.




That's a really good price for those.  I wonder if they are stronger than the ones that are on the vacutainers?  And shipping looks to be only $7.50 no matter if you get 20 or 100.  For that price, I should get a hundred or so and find out!  Does anyone have any experience with these and big needles (say 14 gauge or fatter)?


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: Pastywhyte]
    #26839638 - 07/23/20 12:32 AM (3 years, 6 months ago)

Quote:

Pastywhyte said:
Nice post. I like a good LC lid, often they are useful for a few things. I love LC too but that’s a different story.




Yeah, for example, one thing I like to do is load up a quart jar with 10ml syringes with needles and needle caps on and sterilize the while jar of them.  Then when I want one, I just open the jar, quickly remove one, and close it back fast.  You can do a lot of stuff like that with a good LC lid.


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InvisibleKernale
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26839678 - 07/23/20 01:06 AM (3 years, 6 months ago)

I've found that site has pretty slow shipping, but other than that they are great. They have a few other useful things for this hobby if you look hard enough. You might get a few laughs too; the guy that writes the item descriptions is hilarious.

I haven't actually used them so I can't speak to their quality compared to other ports I've used. But I assume they are the real deal, legit medical surplus, probably high quality material.


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Offlinemushpunx
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26839713 - 07/23/20 02:03 AM (3 years, 6 months ago)

Nice thread! I recently made some of these up to give a try. I used the "autoclave safe" plastic lids you can get off cultivation sites (I think I got mine off FP). I've given these a really good shaking with water in the jar and haven't found them to leak at all without having to use a silicone gasket.

I have some of the heavy duty rubber injection ports but I haven't set them in yet.


Have you ever had a contaminant introduced from pulling syringes up thru the injection port? Do you just sanitize it with ISO first?

Thanks!



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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: mushpunx]
    #26839813 - 07/23/20 05:12 AM (3 years, 6 months ago)

Quote:

mushpunx said:
Nice thread! I recently made some of these up to give a try. I used the "autoclave safe" plastic lids you can get off cultivation sites (I think I got mine off FP). I've given these a really good shaking with water in the jar and haven't found them to leak at all without having to use a silicone gasket.

I have some of the heavy duty rubber injection ports but I haven't set them in yet.


Have you ever had a contaminant introduced from pulling syringes up thru the injection port? Do you just sanitize it with ISO first?

Thanks!






I tend to fill the dimple on top of the injection port with a spray of ISO.  Then when the needle is going in, it passes through it.  When you remove it, it passes through it.  I've never had contamination spread via the route.


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