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OfflineForevergreen
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Condensation on dishes
    #26800536 - 07/02/20 11:58 PM (3 years, 7 months ago)

Last time I placed a few qts of hot water on top of my dishes and it made no difference. This time I stacked them and you can clearly see the condensation



I waited until 120F to pour. Im moving to smaller agar glasses dont worry.

15 gr agar
12 lme
750 ml water. . I boil the mix and pour our the extra after I pour into vodka bottle with hole drilled and filter disc cut to size.


I'll not wrap until dry like last time, which led to every dish getting contammed. Wtf bruh


Edited by Forevergreen (07/03/20 12:02 AM)


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Offlinemushpunx
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Re: Condensation on dishes [Re: Forevergreen]
    #26800601 - 07/03/20 01:32 AM (3 years, 7 months ago)

What do you mean until dry? Until the condensation went away?
Definitely wrap or put into a fresh (sterile) zip lock until you use them.

If you are still getting condensation pour at a little bit cooler than you are already. I wait till it's gonna gel if I wait any longer. You can't waste any time though or it will start pouring glops out.

A little condensation is alright tho.


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OfflineForevergreen
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Re: Condensation on dishes [Re: mushpunx]
    #26800603 - 07/03/20 01:36 AM (3 years, 7 months ago)

I've waited until the point where it was chunky and there was still condensation. Im at a loss for words.

I've tossed over 100 dishes and not one fucking speck of mycelium. I already know its my fault..


Edited by Forevergreen (07/03/20 01:39 AM)


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InvisibleGoatrider
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Re: Condensation on dishes [Re: Forevergreen] * 2
    #26800684 - 07/03/20 04:06 AM (3 years, 7 months ago)

Am i looking wrong, or are there many dishes nearly poured to the rim?
Half the way up would be enough.

I pour also at about 120°F and get a bit condensation.
Then i leave them unwrapped in the SAB for a few days, holes covered up.
Most of it is gone then.
If i don`t need them immediately, i wrap them,
condensation will still be absorbed by agar.

Perhaps you have some heavy temperature swings?
If they get this environment, there`s condensation nonstop.


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Condensation on dishes [Re: Forevergreen]
    #26800815 - 07/03/20 06:36 AM (3 years, 7 months ago)

Some condensation is normal. Pour your plates, let them cool close to room temp, then inoculate. When you inoculate your plates wrap them up immediately after your done. It will usually only be the top couple plates that end up with condensation. Your pour temp is close to where you want it. You want it as cool as you can pour it without it gelling up. I think you are expecting them to pour without any condensation. That won’t happen because of the temp differences. Most of the condensation will subside though.


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OnlineBrian Jones
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Re: Condensation on dishes [Re: Forevergreen]
    #26800846 - 07/03/20 06:54 AM (3 years, 7 months ago)

Condensation did not cause contamination.

I pour at 117 (I think that's Bodhi's tek) and I still get some. He recommends pouring from the bottom of stacks of 10. That reduces most of it. I couldn't do 10 but I'm up to 9. I'm trying to find glasses the right size with flat bottoms. Water that is almost boiling is more effective than hot tap.


--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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InvisibleF. 3
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Re: Condensation on dishes [Re: Brian Jones]
    #26800878 - 07/03/20 07:11 AM (3 years, 7 months ago)

Up with what these guys are saying, there's a ton of agar in your plates, practice to make thin layers.
I had that the first times I did agar and it sucked but it will go away if you gonna keep it away from huge amb temperature swings.
SpitballJedi's write ups about agar you should look in to as well as Bod's.
good luck :peace:


Edited by F. 3 (07/03/20 07:11 AM)


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OfflineJus
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Re: Condensation on dishes [Re: Goatrider]
    #26800889 - 07/03/20 07:18 AM (3 years, 7 months ago)

Quote:

Goatrider said:
Am i looking wrong, or are there many dishes nearly poured to the rim?
Half the way up would be enough.




This, I basically pour the thinnest layer I can for transfer plates.

I get a touch of condensation of a few plates, but it's manageable:



The more agar there is, the more water there is to evaporate and condensate on the colder dish lid.


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InvisibleLotKid
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Re: Condensation on dishes [Re: F. 3]
    #26800893 - 07/03/20 07:19 AM (3 years, 7 months ago)

Why make the agar thin? Thin agar drys out fast. Thicker agar doesnt dry out as fast. I pour my plates pretty hot so the agar isnt chunky. I get plenty of condinsation when i pour them but the condesation goes away after awhile of sitting on the shelf in the lab, waiting to be used.


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InvisibleStrainsfordaze


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Re: Condensation on dishes [Re: LotKid]
    #26800904 - 07/03/20 07:23 AM (3 years, 7 months ago)

:whathesaid:
I’ve never worried about the thickness of the agar.
I pour slightly warmer 120-125f to get a more fluid pour.
Condensation always goes away.


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OfflineJus
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Re: Condensation on dishes [Re: LotKid]
    #26800942 - 07/03/20 07:43 AM (3 years, 7 months ago)

I like to use only as much as I need, and for transfers I've only needed them for a few days.

For storage, yeah, more agar.


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InvisibleF. 3
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Re: Condensation on dishes [Re: LotKid]
    #26801059 - 07/03/20 08:48 AM (3 years, 7 months ago)

Quote:

LotKid said:
Why make the agar thin? Thin agar drys out fast. Thicker agar doesnt dry out as fast. I pour my plates pretty hot so the agar isnt chunky. I get plenty of condinsation when i pour them but the condesation goes away after awhile of sitting on the shelf in the lab, waiting to be used.




Thickness holds heat in. if you want to work with it straight away. and its nice not to have loads of condensation, or need not wait a day or two for it.
planning ahead is the way tho.


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InvisibleLotKid
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Re: Condensation on dishes [Re: F. 3]
    #26801080 - 07/03/20 09:05 AM (3 years, 7 months ago)

Planning ahead... :rockon:


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Offlinemushpunx
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Re: Condensation on dishes [Re: LotKid]
    #26801384 - 07/03/20 12:32 PM (3 years, 7 months ago)

OP you might want to switch to no pours so you can at least get some work done while you figure this out.

Your plates look kinda small already but maybe it's the view, what size are they?

Even if you are getting lots of condensation it shouldn't cause contamination.

I wonder if it's your plates, have they all been from the same source? Did you check the sleeve for leaks, open them only in the SAB, etc?


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OfflineForevergreen
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Re: Condensation on dishes [Re: mushpunx]
    #26801530 - 07/03/20 02:04 PM (3 years, 7 months ago)

I just woke up. Holy shit, tons of responses.

Okay i need to use smaller bottles because its hard as fuck to pour these 60mm dishes without over pouring. I got 60mm because it was cheap for the 500 case. Yes they are in sleeves that seem good. I wipe down the sleeve with ISO before opening in the SAB. I have had 100mm with ridged edges and wish I got those instead. These dishes do suck and I just wanna get thru em.

The condensation seems to have gone and i will inoculate in a few hours.

I appreciate all the responses and after getting super fuckin drunk last night I decided I need to just save up for a flow hood. Ive wiped down all the walls in the house and stepped my cleaning game up 10 levels since i started growing, but its still not enough. Thanks for dealing with me being over frustrated.

Also its hard as fuck to see in my SAB. ive talked about it with someone else. I need to make it higher and get used to looking through the holes or just make eyeholes (rick and morty lol)


Edited by Forevergreen (07/03/20 03:05 PM)


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OnlineBrian Jones
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Re: Condensation on dishes [Re: Forevergreen]
    #26801641 - 07/03/20 03:02 PM (3 years, 7 months ago)

Yeah, I'm thinking about making a bigger SAB and putting in a plexiglass window.

500ml media bottles are easier to work with. Fill up to 400ml and you can pour about 20 100mm dishes. The bigger dishes cost more but I think they're worth it. They're easier to pour into and easier to stack and pour from the bottom of the stack and work up. They give you more surface area to inoculate grain jars. You want to use the plates before the culture reaches the edges.

Bodhi's tek is very useful.


--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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InvisibleLotKid
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Re: Condensation on dishes [Re: Brian Jones]
    #26804871 - 07/05/20 09:25 AM (3 years, 7 months ago)



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Offlineredhandmat
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Re: Condensation on dishes [Re: LotKid]
    #26804877 - 07/05/20 09:30 AM (3 years, 7 months ago)

I always put a large cup with boiling water on top of my plates after I pour them. Also thin agar layer, and I wait for the agar to cool down before pouring.


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Offlinemushpunx
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Re: Condensation on dishes [Re: LotKid]
    #26805152 - 07/05/20 12:15 PM (3 years, 7 months ago)

Quote:

LotKid said:
https://www.eiscolabs.com/products/ch0356set4?_pos=22&_sid=bb65ddce7&_ss=r

I like these way better than media bottles.




How do you use that? Do you have a cover for the top?

I use media bottles and they're alright but I also have Erlenmeyer flasks with threaded caps.

The Erlenmeyer flasks are nice, you don't have to pour the water as deep in the sterilizer and they are a little easier for me to hold.


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OfflineAtmozFear
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Re: Condensation on dishes [Re: mushpunx]
    #26805285 - 07/05/20 01:20 PM (3 years, 7 months ago)

they are propylene so you just put them in the PC with foil, and keep the pitcher covered with the foil, except for the spout, while in the SAB.


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