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Offlinemushroomchaser
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Registered: 07/02/20
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First timer looking for feedback on my process
    #26799643 - 07/02/20 03:15 PM (3 years, 7 months ago)

Hey everyone, total newb here. Just started my first grow a couple days ago using the PF Tek method and wanted to post about it to get some feedback from all you experts. I’m slightly worried about contamination, so if y’all could let me know how you think I did based on the info below, I would really appreciate it!

I also brew beer, so I have an industrial grade sanitizer that I was able to use to clean all my equipment (mixing bowl, jars, lids, measuring cup, etc.) I used Clorox wipes on the counter space, sprayed an air sanitizer, and used a mask and rubber gloves during everything (although I don’t think I sanitized the gloves, oops). My substrate consists of 2/3 cup vermiculite, 1/4 cup water, and 1/4 cup brown rice flour per half pint jar. I packed it down very slightly just on the top before placing the 1/2 inch of dry verm on top (after wiping the top part of the jar with rubbing alcohol). I wrapped the jars with two sheets of aluminum foil each, but did not sanitize the foil. I don’t have a pressure cooker, so I just put the jars in a couple of large covered pots with paper towel on the bottom and several inches of water. Boiled them for a little longer than an hour and then let them sit overnight.

The next morning, I took them out and found that four of them got wet on the inside (I’ll definitely tape the holes next time!) I assume these jars are now worthless, but I still inoculated them just for the hell of it to see what happens. I injected about 1cc of B+ spore solution per jar into the wet substrate, equally divided between the four holes, sterilizing the needle with a basic lighter and rubbing alcohol in between each jar. I only shook the syringe once before inoculating my first jar. About half way through the syringe, I noticed some of the solution was leaking out of a crack in the side whenever I moved the plunger down. Not wanting to waste any of it, I (perhaps foolishly) pushed the drops that fell on the lids into the holes (with gloves on of course). This is where I’m most worried about contamination since I did not sanitize the syringe itself, and the spore solution definitely dripped down the side of it. I did sanitize the lid though. I’m also wondering whether getting the dry verm layer wet with spore solution is ok?

I covered all the holes with microtape, and the jars are now sitting in an 80 degree room without direct light. I’m keeping the four wet jars away from the good ones just in case. It’s only been 2.5 days since inoculation, but of course I’m nervous and impatient lol. No signs of any growth of anything yet, which I know is normal. But fuck, that syringe leak is making me nervous lol. What are people’s thoughts?


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OfflineEdmunter
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Registered: 05/01/13
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Re: First timer looking for feedback on my process [Re: mushroomchaser]
    #26799674 - 07/02/20 03:29 PM (3 years, 7 months ago)

The important thing is to follow the TEK to the T and make sure all inoculation points MUST be at least sanitised but proper practice is to flame the syringe, wipe the inoculation point  but best imo to put microtape on that b4 sterilising inject through that and add another layer :shrug:


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