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AlpineHighs
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Sour oat jars ==> Spawn???
#26799144 - 07/02/20 11:56 AM (3 years, 6 months ago) |
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I was planning on spawning 12 MS oat quarts to bulk today, but when I shook and opened them there is a strong sour smell. I only see a couple very small discolored portions in the grain though.
I have coir/verm ready to go right now for X2 54qt tubs. I also just removed from the fridge 12 fully colonized clone/pin clone jars that had been in there about 6 hours. I opened one and detected no sour smell.
I've been frantically reading threads on pros/cons of spawning bacterial grain, and I'm at a loss of how to proceed...
Is it even remotely worth trying to fruit the sour MS jars? Or should I not risk contaminating my small basement apartment? I have access to a separate storage area that could potentially be home for 2 dirty monotubs?? Can I let my 12 clean clone jars warm up for a couple hours and spawn them to the substrate I've prepped? Can I spawn the clean jars while they're still cold? Should I toss all this substrate and make new sub in couple days when the jars are fully room temp?
Big bummer Damage control advice is appreciated
Edited by AlpineHighs (07/02/20 11:58 AM)
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AtmozFear
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799180 - 07/02/20 12:10 PM (3 years, 6 months ago) |
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all i know is bacteria smells much worse than mold or any other fungi... that said, sour isn't good.
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Roger Clemency
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799181 - 07/02/20 12:10 PM (3 years, 6 months ago) |
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If it's feasible where you live you could spawn them in an outdoor patch. I've had bacterial jars F'n explode outside. I just dug a 3-4 inch deep square around tub size, mixed untreated hpoo and verm with the spawn and watered, left a little tarp over it for a while until it started pinning. Coir or whatever would work just as well i'm sure.
You could try top fruiting them.
I'd do the outside and save the bulk sub for the clean jars but it's really up to you and depends how bad the jars actually are.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Crackatoa
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799194 - 07/02/20 12:15 PM (3 years, 6 months ago) |
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Without seeing the jars it's hard to say. There's a good chance you can get a fruit or two from bacterial spawn. Outdoor would be great or top fruit. If it was trich I would say to throw away. Yeah, you can let them warm up or cold it'll be fine. Putting spawn to hot substrate is a no go. If you're using coir as substrate. It can last for weeks. I always keep a brick ready to go in my bucket. I use it every few days but it has sat for weeks
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AlpineHighs
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Re: Sour oat jars ==> Spawn??? [Re: Crackatoa]
#26799251 - 07/02/20 12:36 PM (3 years, 6 months ago) |
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Quote:
Roger Clemency said: If it's feasible where you live you could spawn them in an outdoor patch. I've had bacterial jars F'n explode outside. I just dug a 3-4 inch deep square around tub size, mixed untreated hpoo and verm with the spawn and watered, left a little tarp over it for a while until it started pinning. Coir or whatever would work just as well i'm sure.
You could try top fruiting them.
I'd do the outside and save the bulk sub for the clean jars but it's really up to you and depends how bad the jars actually are.
I definitely cant fruit them outside. My land lady would flip
 Didnt shake one of them. Tough to get a good pic, but its a blue spot on top of the grains. Unsure if its trich, but the shaken jars are way pungent sour.
Since I shook all of them and cant top case them today, I think I'm gunna use one or two backup monotubs, mix all this dirty spawn with substrate I was going to use for one unBODified tub, and stash them in the segregated storage area of the basement I'm in. But if you guys can confirm its trich on that grain, should I toss it all ASAP?
I'll plan to spawn my clean jars in a couple hours after they warm up. I had the 12 MS quarts in my fridge for > a week over vacation, and I dont want to chance the possibility that the fridge is the source of this contam.
Dangit
Edited by AlpineHighs (07/02/20 12:37 PM)
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AlpineHighs
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799561 - 07/02/20 02:40 PM (3 years, 6 months ago) |
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False alarm. All 45 jars = fucked. Back to the board of drawing
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Roger Clemency
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799623 - 07/02/20 03:08 PM (3 years, 6 months ago) |
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When you say MS do you mean a spore syringe?
MS is multispore, which could refer to a spore syringe but also just spores germinated on agar, cleaned up, and then used to nocc something up. A clone or monoculture would not be considered MS.
If you havent been cleaning up on agar first you should make that the start of your next grow. I'm almost hesitant to give that advice to people now only because I'm saying it like 10 times a day as if it's a cure all but it really is step #1 in my opinion.
Spores straight to grain does work, even fairly often for some people, but its like walking through a cluttered garage in the dark. Sometimes you make it through fine, other times you smash into something halfway..or worse right before the end. Agar be the light.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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AlpineHighs
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Nah, agar is the best advice
I stumbled through my first attempt at mycology in February inoculating rye grain with a PE spore syringe, and somehow harvested and have been enjoying like +100g dry from a bush league monotub. It took 4 months start to fruit, and I was working on PF B+ in the meantime. Successfully put B+ clones and pins to agar, transferred until they were gorgeous cultures. Also got a bunch of clean spore plates transferred from the same B+ syringe. Inoculated 45 jars of rye and oats with clean B+ mycelium, and was seeing awesome colonization.
Then, I had to move into the basement last month. Upstairs is a super hot climate, basement is constant 60 degrees. Spent > week filling all zones with filthy air while cleaning. Half my jars were covered in pine pollen from a window my former roommate left open. Then I went on vacation, put 1/2 jars in a filthy fridge to chill while the other 1/2 finished colonizing. Was pumped to spawn today, and was slapped in the face with sour puss
I'm currently a bit too drunk and bummed to venture legit guesses, but maybe multiple temp variations + overwhelming filthy air + overly saturated grains + whatever the fuck else fucked all my spawn
Who knows
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Roger Clemency
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26799726 - 07/02/20 03:55 PM (3 years, 6 months ago) |
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Damn man that sucks. Check out Munchauzen's blenderless LI TEK. Maybe try that with a handful of jars to see if it works for you. If you have a clean agar plate now you could have more spawn ready in 10-14 days.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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AlpineHighs
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Yeah that LI TEK sounds pretty legit. Luckily I have some PE plates from my first grow but they're all originals. Gunna pour a shitload of plates tonight and start transferring.
What's your method for spore syringe to agar? I've tried the basic one droplet method, several drops, and used an inoculation loop, but I've only germinated a tiny spot on 1 plate out of 10-20 each time. Hoping to improve my technique. Or maybe it's an agar recipe thing?
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panne cyanne
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26801859 - 07/03/20 05:15 PM (3 years, 6 months ago) |
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every time i hit a sour one i just trash em. there is no winning agianst bacteria.
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CrashTest
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26801860 - 07/03/20 05:16 PM (3 years, 6 months ago) |
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Quote:
AlpineHighs said: Yeah that LI TEK sounds pretty legit. Luckily I have some PE plates from my first grow but they're all originals. Gunna pour a shitload of plates tonight and start transferring.
What's your method for spore syringe to agar? I've tried the basic one droplet method, several drops, and used an inoculation loop, but I've only germinated a tiny spot on 1 plate out of 10-20 each time. Hoping to improve my technique. Or maybe it's an agar recipe thing?
Also in for this info because I want to start agar with a b+ MSS
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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Roger Clemency
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Re: Sour oat jars ==> Spawn??? [Re: CrashTest]
#26802187 - 07/03/20 07:56 PM (3 years, 6 months ago) |
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I'm always trying to steer people away from syringes unless it's for BRF jars. I've had trouble with every one i've tried to germinate on agar and I see the same thing with a lot of people posting here.
One idea that can work if you're set on using that syringe is to inoculate a little jar with some sterilized grain in it. Something small and easy to reach into with tweezers. Spores that can't get started on agar seem to be able to at least germinate and colonize some grains, and you can then pluck that grain out and put it on a plate and clean it from there.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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CrashTest
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Quote:
Roger Clemency said: I'm always trying to steer people away from syringes unless it's for BRF jars. I've had trouble with every one i've tried to germinate on agar and I see the same thing with a lot of people posting here.
One idea that can work if you're set on using that syringe is to inoculate a little jar with some sterilized grain in it. Something small and easy to reach into with tweezers. Spores that can't get started on agar seem to be able to at least germinate and colonize some grains, and you can then pluck that grain out and put it on a plate and clean it from there.
If I have a spore print from a fruited brf cake, how do you transfer that into agar ? Just scrape the spores with a sterile tool and transfer to agar?
--------------------
this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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Crackatoa
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Re: Sour oat jars ==> Spawn??? [Re: CrashTest] 1
#26802218 - 07/03/20 08:19 PM (3 years, 6 months ago) |
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Pretty much, I use a sterile swab to transfer.
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AlpineHighs
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Great idea. Next time (hopefully soon) I'm PCing grain jars I'll put some in a 1/2 pint mason from PF tek and squirt some spores in there
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AlpineHighs
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Just had a thought...
I've been pouring plates and inoculating with spores in the same session. I'd say I wait 45ish min or so for the plates to solidify. If mycelium dies after a while around 86F degrees, could spores be affected at or above this temp too? I pour agar around 117F from a hot water bath, and it takes over an hour to reach that temp out of the PC. Probably takes quite a while for freshly poured plates to get below 86F...?
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Crackatoa
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Re: Sour oat jars ==> Spawn??? [Re: AlpineHighs]
#26802440 - 07/03/20 10:45 PM (3 years, 6 months ago) |
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Could be an issue. I wait overnight or s couple days for any contamination to show it's ugly face
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