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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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Question about old spores 1
#26798050 - 07/01/20 11:01 PM (3 years, 6 months ago) |
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I have panaeolus cyanescens prints that are 2 1/2 year's old. Should I rehydrate the spores before I put them on agar? Do you think I'll get growth without rehydrating them? For the most part they've been in the refrigerator the whole time. Thanks
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,159
Loc: rural ghetto
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Re: Question about old spores [Re: Shenmue]
#26798168 - 07/02/20 12:24 AM (3 years, 6 months ago) |
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People have been talking about forced rehydration using a rubberband and a syringe, I dunno how that works.
Never really had any germination when prints go for more than a year in room temp. But it might also be because of our very arid climate. Your spores might very well be viable because you stored it in the fridge. Id say streak some on agar and see for yourself.
--------------------
Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Question about old spores [Re: pablokabute]
#26798355 - 07/02/20 02:31 AM (3 years, 6 months ago) |
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Thats interesting. Does the rubber band go around the syringe and place pressure on the spores/syringe? 
OP, i would hydrate your spores in a syringe if i were u.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
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As far as I understand it where I've seen people talk about it is that the rubber band holds the plunger down creating pressure in the syringe. Which would then force the water in the syringe into the spores.
Logically, it makes sense. I have no idea if it works better than just normal rehydration lol. It would be interesting to measure spore size before and after and compare regular hydration vs. that syringe hydration. That also may be an awful way to test it lol
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mushpunx
Fungus Punk



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Re: Question about old spores [Re: Gan] 1
#26798764 - 07/02/20 08:09 AM (3 years, 6 months ago) |
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I've never kept my prints in the fridge
I guess we shall see man, I just put some Panaeolus Bisporus on agar this morning, the print is at least 3 1/2 years old. I just put the on agar with a loop. I didn't hydrate
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (07/02/20 08:10 AM)
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The Mycologist
Explorer

Registered: 05/06/16
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Last seen: 29 days, 4 hours
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Quote:
LogicaL Chaos said: Thats interesting. Does the rubber band go around the syringe and place pressure on the spores/syringe? 
OP, i would hydrate your spores in a syringe if i were u.
Yes thats what they say.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Snaggletoots
One pump stumpy

Registered: 06/26/20
Posts: 123
Loc: A nut among washers
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Could you cap your half full syringe and pull the plunger back, then just wedge something or tape it there , creating a negative? Would that be the same as the rubber band wrapping?
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The Mycologist
Explorer

Registered: 05/06/16
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No it would be the opposite
You want the contents to be under higher than normal pressure.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Snaggletoots
One pump stumpy

Registered: 06/26/20
Posts: 123
Loc: A nut among washers
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Positive pressure, okay thank you kindly
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Shenmue
Dark Lord of the Sith
Registered: 12/21/18
Posts: 2,514
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Thanks guys. I'll go ahead and hydrate them..
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