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OfflineCatsLoveHouseMusic
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Just did first G2G. Im alot more comfortable with agar to grain
    #26795825 - 06/30/20 10:35 PM (3 years, 7 months ago)

Granted it was my first time, well see how it goes. Im a freak about santizing everything, multiple times, wearing my N95 mask, etc... I just felt agar felt safer? Its just super easy IMO. I did 1 Jar to 5 receiving jars, just to make it go faster... Make sure you have a tall SAB, isopropyl under the rim really good.


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OfflineAeser
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26795894 - 06/30/20 11:08 PM (3 years, 7 months ago)

Good luck! Agar and G2G is a big upgrade. Changed my whole mushy world after doing lame BRFs.


--------------------
Anxiety is when your stuck in the future.
Depression is when your stuck in the past.
Happiness is when your stuck in the present.


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InvisibleStipe-n CapMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26795975 - 07/01/20 12:05 AM (3 years, 7 months ago)

Quote:

CatsLoveHouseMusic said:
Granted it was my first time, well see how it goes. Im a freak about santizing everything, multiple times, wearing my N95 mask, etc... I just felt agar felt safer? Its just super easy IMO. I did 1 Jar to 5 receiving jars, just to make it go faster... Make sure you have a tall SAB, isopropyl under the rim really good.




Damn, those 5 jars are going to finish fast. I do 1:10 and they're done in 7-8 days.


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OfflineCatsLoveHouseMusic
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Stipe-n Cap]
    #26796005 - 07/01/20 12:25 AM (3 years, 7 months ago)

I wanted them to finish fast, I will then do like 7-8 jars next time and see how it goes. It was my first time so I was thinkijg maby if I messed up somehow the contam would show quicker. These jars were inoculated with 5th transfer agar too... hopefully they turn out clean.


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InvisibleStipe-n CapMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26796016 - 07/01/20 12:33 AM (3 years, 7 months ago)

It's pretty easy to do I'm sure that you're fine. As long as you don't pass anything other than the mouth of your master over the receiving jar it will work out.


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Offlineorange771
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Stipe-n Cap]
    #26796149 - 07/01/20 03:11 AM (3 years, 7 months ago)

My experience has been that in my SAB, with a lot less precautions than you take all of my agar to grain works out great. I've literally never had one fail on me but I have good sterile procedures. My G2G from A2G masters will occasionally throw some contams my way, but I feel it's due to the fact that I don't go the extra mile with air sanitizers, showering before and doing SAB work naked like some of you guys. If you can pull off A2G you understand the principles of a SAB and you will be fine.


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OfflineA.k.aM
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: orange771]
    #26796474 - 07/01/20 08:05 AM (3 years, 7 months ago)

I had problems getting the last half of the grain out cuz the jar would hit the roof so I took a styrofoam block from an amazon box and cut it so that i can lean the receiving jar on it which opens up enough space to pour the whole jar out.

Plus frees up a hand.


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LAGM2020


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InvisiblePastywhyteMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: A.k.a]
    #26796578 - 07/01/20 09:16 AM (3 years, 7 months ago)

I like to use pint jars as masters when I do g2g. 1 pint to 6 quarts is no different than a quart to 12 quarts. However the pint jar is easier to handle and only needing to have six jars going per master makes it easier to maneuver in a tight SAB or if you have a small flowhood.


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OfflineCatsLoveHouseMusic
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Pastywhyte]
    #26797387 - 07/01/20 04:23 PM (3 years, 7 months ago)

Good call on using pints. I had trouble hitting the roof of my SAB too, as the poster above said. Need a taller SAB, but I only wasted a small amount of grain/mycelium. In all reality, all of this is very simple and straight forward. It just feels difficult the first time and Im so lazy after work. I have too much agar and grains going lol...


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OfflineNobodycare
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26798014 - 07/01/20 10:41 PM (3 years, 7 months ago)

Ive never done agar but i find g2G to be super easy and never had an issue despite being somewhat inexperienced? I do 1 qt master to 7 qt jars. Way faster than syringe to grain. People hate on MS but all my grows have been pretty consistently fire.


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InvisibleStipe-n CapMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Nobodycare]
    #26798036 - 07/01/20 10:52 PM (3 years, 7 months ago)

People don't hate on MS, they caution against spores to grain and for good reason.


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OfflineLogicaL ChaosM
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26798352 - 07/02/20 02:29 AM (3 years, 7 months ago)

I would argue that G2G is one of the easiest procedures in the hobby! :cheers: Enjoy!


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OfflineCatsLoveHouseMusic
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: LogicaL Chaos]
    #26798822 - 07/02/20 08:41 AM (3 years, 7 months ago)

Everything has been pretty simple up to this point, including g2g. I just worry that my precautions or whatever werent enough. I only did 5 jars from the 1 master and they are pretty far along already. Even if there was enough bacteria to contam a jar, since the myc is growing so fast would that make is “less susceptible” to being contamed and thrown out?

If these 5 jars colonize without any contam, Ill have ao many grain jars colonized soon.


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OfflineNobodycare
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26798836 - 07/02/20 08:47 AM (3 years, 7 months ago)

I have had some bacterial issues. Moreso when I started bc of my WBS moisture content. Ive worked to correct this but still do every now and then but theyve always grown fine and not gotten contamned.


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OfflineCatsLoveHouseMusic
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Nobodycare]
    #26798850 - 07/02/20 08:51 AM (3 years, 7 months ago)

For sure. I havnt had much issue with my grain jars as of yet. Were your jars too wet? I had my first set of jars that were too wet, I let em sit for 3 days and shook them a few times in between.


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OfflineNobodycare
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: CatsLoveHouseMusic]
    #26798859 - 07/02/20 08:56 AM (3 years, 7 months ago)

Yeah...I think the issue was too much water on the outside surfaces of the seed. I wasnt letting them dry long enough before loading. Now I let them cool/dry for about 6 hours and it seems to have helped. Think I got some slight bacteria in my current jars that are almost done. I always thought it was rhizo but people corrected me and said its bacterial.


Edited by Nobodycare (07/02/20 08:57 AM)


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OfflineNobodycare
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Nobodycare]
    #26798873 - 07/02/20 09:04 AM (3 years, 7 months ago)

Yeah, here is one of those current jars I was talking about. Those string like "fingers" are supposed to indicate bacteria. Ive had worse that grew just fine so I'm not too worried.



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InvisiblePastywhyteMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Nobodycare]
    #26798884 - 07/02/20 09:10 AM (3 years, 7 months ago)

Quote:

Nobodycare said:
Yeah, here is one of those current jars I was talking about. Those string like "fingers" are supposed to indicate bacteria. Ive had worse that grew just fine so I'm not too worried.






Those are just rhizomorphic growth. See how they’re growing in an organized manner? That’s fine. It’s when they’re splayed out every which way that you suspect contams.


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OfflineNobodycare
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Pastywhyte]
    #26798929 - 07/02/20 09:46 AM (3 years, 7 months ago)

I see what you mean. These are the ones i was told were bacterial. They look almost the same to me. These are just way more pronounced and the strings are longer. Even these jars grew just fine. Glad to hear its actually rhizo this time.



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InvisiblePastywhyteMDiscord
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Re: Just did first G2G. Im alot more comfortable with agar to grain [Re: Nobodycare]
    #26798957 - 07/02/20 10:05 AM (3 years, 7 months ago)

Yes those are much more suspect. It’s a fine detail but a practiced eye can pick it out. Also I wouldn’t ever hesitate to spawn jars like those. I just might not use them to G2G.


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