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Offlineagar_id
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Registered: 07/01/20
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Cubensis growth or contamination on agar?
    #26796270 - 07/01/20 05:32 AM (3 years, 6 months ago)

I have started using agar to spawn spores from a syringe for the first time. I spawned what I thought was mycelium and have transfered three times now.
However, now that I am getting ready to inoculate my grain jars with an agar wedge I am second guessing whether this is indeed cubensis or some contamination, as there are some visual differences to other agar posts that I have seen on the forum.
Would anyone be able to tell me whether this is indeed cubensis, or have I been carefully culturing and isolating a sneaky bacterial colony?
I have attached some pictures. The agar is dyed blue so it is hard to capture an image, but I have taken some against a light. The dark specks are bits of malt extract. Please let me know if I should take some different pics.


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,670
Re: Cubensis growth or contamination on agar? [Re: agar_id]
    #26796364 - 07/01/20 06:59 AM (3 years, 6 months ago)

It's a guess without light on the surface.


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Offlineagar_id
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Last seen: 3 years, 6 months
Re: Cubensis growth or contamination on agar? [Re: agar_id]
    #26796447 - 07/01/20 07:51 AM (3 years, 6 months ago)

I see, these are the best I could manage. Some have been used for transfers, which is why they have a bit of black on them from the surgical blade char.


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InvisibleLadysKnight
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Re: Cubensis growth or contamination on agar? [Re: agar_id]
    #26796457 - 07/01/20 07:56 AM (3 years, 6 months ago)

"have I been carefully culturing and isolating a sneaky bacterial colony?"

You called it


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Offlineagar_id
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Registered: 07/01/20
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Last seen: 3 years, 6 months
Re: Cubensis growth or contamination on agar? [Re: LadysKnight]
    #26796492 - 07/01/20 08:22 AM (3 years, 6 months ago)

Ah thats a real shame, I was really struggling to get anything to germinate on my plates, had some sitting around with no growth of any kind for over two weeks. So when some white growth appeared I must have seen what I wanted to see.
Thanks for the reply


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