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 krock
Krockodile
Registered: 12/18/19
Posts: 153
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 Interesting agar recipes from the Radical Mycology book   3
#26795122 - 06/30/20 07:56 PM (1 year, 3 months ago) |
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I recently posted some agar recipes from the Radical Mycology book in the agar envy thread and D3 suggested I should put it in a new thread so that it can be found easily with the forum search.
This is how the recipes are presented in the book:
I made a transcript for better search indexing:
Agar Media Recipes
Unless noted, all agar recipes start with 500 milliliters of water and 10 grams of agar. Never use the following antifungal ingredients in media recipes: coconut, garlic, pomegranate, cinnamon, cloves, curry, and cilantro.
COMMONLY USED MEDIA
Malt Extract Agar (ME)
• Dry, light malt (barley) extract - 10 g
Malt Yeast Extract (MYA)
• Malt - 10 mL
• Yeast - 1 mL
Malt Extract with Activated Carbon (MEAC)
• Malt - 10 g
• Activated carbon - 1 g
Malt Extract with Peptone (MEP)
• Malt - 10 g
• Peptone - 1 g
Malt Extract with Yeast and Peptone (MYAP)
• Malt- 10 g
• Yeast - 1 g
• Peptone - 1 g
Malt Extract with Yeast, Peptone, and Activated Carbon (MEPYAC)
• Malt - 10 g
• Peptone - 1 g
• Yeast - 1 g
• Activated carbon - 1 g
Malt Extract with Milo (MEM)
• Malt- 10 g
• Ground milo - 10 g
Malt Extract with Corn (MEC)
• Malt- 10 g
• Ground corn - 10 g
Malt Extract with Honey (MEH)
• Malt- 10 g
• Honey- 16 g
ATTC Medium 597
• Malt - 3.75 g
• Yeast - 0.3 g
• Peptone - 0.5 g
MYAP with Multi-vitamin (MYMP)
• Fresh ground crickets - 8 g
• Multi vitamin - 0.01 g
• Peptone - 1 g
• Yeast - 1 g
• Malt- 10 g
Add multi-vitamin after sterilization
MYAP with elements (MYPMCZ)
• Yeast - 1 g
• Peptone - 1 g
• Calcium - 0.01 g
• Magnesium - 0.01 g
• Zinc - 0.01 g
MYA with Cordyceps sinensis (CS4MY)
• Malt - 3.75 g
• Yeast - 0.3 g
• Ground Cordyceps sinensis 4 - 10 g
Potato Dextrose Agar (PDA)
• Diced and boiled potatoes - 150 g
OR Potato starch - 10 g
• Dextrose - 7 g
In 500 ml of water, boil 150 g of potatoes. Strain out the solids, bring the solution back to 500 ml with water, then add remaining ingredients. Five grams of potato flakes can be used as a substitute.
Potato Dextrose Agar (PDA)
• Dextrose or Honey/Corn syrup - 7 g or 10 ml
• Diced potatoes - 150 g
Potato Dextrose Yeast Agar (PDYA)
• Dextrose or Honey/Corn syrup - 7 g or 10 ml
• Diced potatoes - 150 g
• Yeast - 1 g
Potato, Ammonium citrate, Thiamin Agar (PAT)
Cordyceps militaris is known to fruit from this
media when used to inoculate brown rice.
• Potato broth - 100 ml
• KH2PO4 - 1 g
• MgSO4 - 0.5 g
• Peptone - 1.5 g
• Ammonium citrate - 0.5 g
• Dextrose - 15 g
• Thiamine (Bl vitamin) - 0.025 g
Potato Skin Dextrose Agar (PSDA)
• Dried potato skins - 10 g
• Dextrose - 7 g
Barley Flour Malt Extract Agar (BFMA)
• Barley flour - 40 g
• Malt extract - 2 g
• Yeast extract (optional) - 1 g
Oatmeal Agar (OA)
• Filtered, cooked oatmeal - 30 g
Cornmeal Malt Agar (CMMA)
• Cornmeal - 10 g
• Dextrose - 7 g
Dog Food Agar (DFA)
• Dried, ground dog food - 10 g
• Amaranth flour - 10 g
• Dextrose or malt extract - 2 g
Amaranth Soy Agar (ASA)
• Amaranth flour - 20 g
• Soy flour - 20 g
EntheoGenesis Agar (EA)
• Amaranth flour - 20 g
• Soy flour - 20 g
• Brown rice flour - 10 g
• Potato flour - 10 g
• Malted barley - 2 g
Moonflower's Rice Malt-Alfalfa-Brewer's Yeast Agar (MRMABYA)
Soak 1 cup of alfalfa and 2 cups of rice in 1.5 quarts of clean water for 2 hours at room temp. Stir occasionally. Filter out solids. Pour a packet of baker's yeast in water. After 30 minutes, filter out the solids. Combine liquids and add 1 tablet of crushed dolomite. Supports luxuriant mycelial growth and germinates spores.
Spore Germination Agar# 1
• Cornmeal - 10 g
• Dextrose - 1 g
• Yeast - 0.5 g
Spore Germination Agar #2
• Cornmeal - 10 g
• Dextrose - 3.5 g
• Sucrose - 5 g
• Yeast - 0.5 g
• KH2PO4 - 0.5 g
Spore Germination Agar #3
• Cornmeal - 10 g
• Malt - 0.8 g
Spore Germination Agar #4
• Cornmeal - 10 g
• Glucose - 1 g
• Sucrose - 1.5 g
• Yeast - 0.5 g
JUICE / BROTH MEDIA
VB Juice Agar (VBA)
• CaCO3 - 1.5 g
• V8 Juice - 100 mL
Beef Broth Agar (BBA)
• Beef broth - 20 mL
• Malt - 5 g
• Yeast - 1 g
Chicken Broth Agar (CBA)
• Chicken broth - 20 mL
• Malt - 5 g
• Yeast - 1 g
Butternut Squash Soup Agar (BNSA)
• Butternut squash soup - 20 mL
• Malt - 5 g
• Yeast - 1 g
Beef Yeast Agar (BYA)
• H2O - 300 mL
• Yeast - 2 g
• Beef broth - 200 mL
Malt with Activated Carbon and KOH (R7)
• Epsom salts - 0.25 g
• Activated carbon - 1.3 g
• Malt - 12.5 g
• Humus - 2.5 g
• KOH 1 % - 20 mL
CHEMICAL MEDIA
Organic Medium Agar (OMA)
• Glucose - 5 g
• Peptone - 0.5 g
• Yeast - 0.05 g
• KH2PO4 - 1 g
• MgSO4 • 7H2O 0.15 g
• OR MgSO4 - 0.075 g
Czapek's Medium
• NaNO3 - 1.5 g
• K2HPO4 - 0.5 g
• MgSO4 - 0.25 g
• KCl- 0.25 g
• FeSO4 - 0.0187 g
OR Fe SO4 • 7H2O - 0.0094 g
• Dextrose - 15 g
Used for the isolation and culture of saprobic soil
microorganisms.
ANIMAL RELATED MEDIA
Blood Worm Agar (BW)
• Blood worms - 5 g
Krill Agar (KA)
• Krill - 10 g
Fish Flake Dextrose Agar (FDA)
• Fish flakes - 3 g
• Dextrose - 10 g
Meal Worm Agar (MWA)
• Meal worms (ground) - 10 g
• Dextrose - 3 g
• Yeast - 1 g
Fishy Wax Agar (FWA)
• Bee's wax - 1 g
• Krill, tubiflex worms,
and fish flakes - 3.3 g each
MISCELLANEOUS MEDIA
Manure Agar
• Ground horse manure - 50-60 g
Boil manure in water for 10 minutes, let sit for
16-20 hours, filter out solids, bring back to 500
mL and add agar.
Compost Agar
Air-dry and then grind hot compost obtained
during its peak state. Mix 50 g of this dried
compost powder to 750 mL of water. PC for
1 hour at 15 psi. Filter twice through cheese
cloth. Bring volume up to 500 mL. Add 10 g
agar and PC as normal.
Complete Media (CM)
• Sucrose - 15 g
• Ammonium tartrate (NH4 -) - 2.5 g
• Ammonium nitrate (NH4NO3) - 0.5 g
• Magnesium sulfate heptahydrate
• (MgSO4 • 7H2O) - 0.25 g
• Sodium chloride (NaCl) - 0.5 g
• Calcium chloride (CaC1 2 ) - 0.065 g
• Yeast - 0.5 g
• Monopotassium phosphate (KH2PO4) - 0.5 g
Richard's Medium (forT. harzianum)
• Potassium nitrate - 5 g
• Monopotassium dihydrogen phosphate - 2.5 g
• Magnesium sulphate - 1.25 g
• Ferric chloride - 0.01 g
• Sucrose - 25 g
wMY Agar (For Protostelid slime molds)
• Malt extract - 0.01 g
• Yeast extract - 0.01 g
• Dibasic potassium phosphate - 0.375 g
Distilled Water Agar (DWA)
This formula should be made solely with dis-
tilled water and agar.
Sea Water Agar (SWA)
• H2O - 125 mL
• Agar - 10 g
• Sea water - 375 mL
• Beef extract - 5 g
• Peptone - 5 g
Dissolve the beef extract and peptone in warmed HP. Adjust the pH to 7.8 and boil for 10 minutes. Readjust pH to 7.3 and add agar.
Honey Agar (HON)
• Honey- 16 g
Beet Root Agar (BRA)
• Beet root supplement - 3 g
• Malt - 10 g
Peaches Malt Agar (PMA)
• Canned peaches mash - 10 g
• Dextrose - 7 g
Spirulina Agar (SPA)
• Spirulina supplement - 3 g
• Malt - 10 g
Nori Agar (NorA)
• Nori- 3 g
• Malt - 10 g
Brine Agar (BA)
• Brine - 10 g
Bean Soup Agar (BSA)
• H2O-400 mL
• Agar - 10 g
• Bean soup - 100 mL
Carrot Malt Agar (CMA)
• Carrot mash - 20 g
• Malt - 5 g
Sunflower Seed Agar (SFS)
• Ground sunflower seeds - 10 g
Almond Agar (AA)
• Ground almonds - 10 g
Edited by krock (07/01/20 06:15 AM)
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 tripdawg420
low life with no life
Registered: 02/03/09
Posts: 6,967
Loc: illinois
Last seen: 23 hours, 11 minutes
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 Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#26795264 - 06/30/20 08:55 PM (1 year, 3 months ago) |
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 Tight Lunchbox
Drunk cat
Registered: 11/06/16
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Re: Interesting agar recipes from the Radical Mycology book [Re: tripdawg420]
#26795520 - 06/30/20 10:35 PM (1 year, 3 months ago) |
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Thanks, OP.
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"it's all a joke between mom contractions and coffin fittings"
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mushpunx
Fungus Punk
Registered: 04/20/14
Posts: 13,391
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Re: Interesting agar recipes from the Radical Mycology book [Re: Tight Lunchbox]
#26796037 - 07/01/20 02:53 AM (1 year, 3 months ago) |
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This is pretty cool. My memory is shot and I frequently find myself looking up how much MEA to use per litre 
The germination blends are interesting. Sometimes spores are so stubborn, I'm willing to try anything
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Amateur Mycologists United
AMU Q&A
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sandman420
Saint PP
Registered: 06/17/04
Posts: 3,379
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Re: Interesting agar recipes from the Radical Mycology book [Re: mushpunx]
#26796349 - 07/01/20 08:46 AM (1 year, 3 months ago) |
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Imagine your wife catching you boiling 50 grams of horse shit on the stove lmao.
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Have no fear of perfection, you'll never reach it.
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krock
Krockodile
Registered: 12/18/19
Posts: 153
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Re: Interesting agar recipes from the Radical Mycology book [Re: sandman420]
#26796412 - 07/01/20 09:24 AM (1 year, 3 months ago) |
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Quote:
Imagine your wife catching you boiling 50 grams of horse shit on the stove lmao.
LOL!!
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Jus
Stranger
Registered: 09/27/19
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Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#26796427 - 07/01/20 09:38 AM (1 year, 3 months ago) |
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Wow, some of these are so much work compared to others.
That butternut squash soup agar sounds delicious though.
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amy437737
Ranger
Registered: 05/01/21
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Loc: LA
Last seen: 2 months, 28 days
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Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#27293016 - 05/03/21 11:06 PM (5 months, 12 days ago) |
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Thank you so much for this post. I am researching agar now to do a test and this was so helpful.
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-Amy
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Benson
The Kidd ⭐
Registered: 09/29/20
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Re: Interesting agar recipes from the Radical Mycology book [Re: amy437737]
#27293022 - 05/03/21 11:09 PM (5 months, 12 days ago) |
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Dope, thanks for transcribing it too 
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amy437737
Ranger
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Loc: LA
Last seen: 2 months, 28 days
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Re: Interesting agar recipes from the Radical Mycology book [Re: Benson]
#27294954 - 05/05/21 04:49 AM (5 months, 11 days ago) |
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I made a few of these including the horse manure agar. Testing seven different syringes on LME, PDA (PDA is a Philly GT recipe), BRF, a mix of extras, HorsePooH20 and GrainSoakWater agar.
Sterilized in an All American and poured in a SAB.
I'm a noob. Got no idea what I am doing.
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-Amy
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fungilove
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Re: Interesting agar recipes from the Radical Mycology book [Re: amy437737]
#27364637 - 06/27/21 12:41 AM (3 months, 20 days ago) |
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I'm wondering what came of this test?
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B H O
Registered: 03/17/13
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Re: Interesting agar recipes from the Radical Mycology book [Re: fungilove]
#27364640 - 06/27/21 12:47 AM (3 months, 20 days ago) |
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Quote:
fungilove said:
I'm wondering what came of this test?
Right!? It’s been a month and no feedback?
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kanemush
Grumpy
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Re: Interesting agar recipes from the Radical Mycology book [Re: B H O]
#27364661 - 06/27/21 01:12 AM (3 months, 20 days ago) |
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interesting I think I may try some of these out thank you for posting.
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multifractal
Student
Registered: 05/09/15
Posts: 816
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Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#27365739 - 06/28/21 02:15 AM (3 months, 19 days ago) |
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lol brine agar? these are cool. I mixed up agar using the cornmeal/dextrose/yeast spore germination recipe today. I'll see how it goes.
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Edited by multifractal (06/28/21 02:16 AM)
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doubleD
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Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27365839 - 06/28/21 06:35 AM (3 months, 19 days ago) |
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damn, good luck amy! thats a lot to keep track of
i may try something here if i hear good things about a recipe, but i usually use what i got. about 12.5g agar for 25 plates and then a couple gs of lme and a splash of nutritional yeast, and sometimes tea as a water substitute
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fungusul
Fungus Kingdom
Registered: 07/16/20
Posts: 299
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Re: Interesting agar recipes from the Radical Mycology book [Re: doubleD]
#27369419 - 06/30/21 08:29 PM (3 months, 16 days ago) |
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Thanks OP! 
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0ptiquest
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Re: Interesting agar recipes from the Radical Mycology book [Re: fungusul]
#27397980 - 07/22/21 02:37 PM (2 months, 25 days ago) |
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Someone have tried the spore germination recipes?
Will try some dextrose in my next plates because i have it.
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multifractal
Student
Registered: 05/09/15
Posts: 816
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Re: Interesting agar recipes from the Radical Mycology book [Re: 0ptiquest]
#27398192 - 07/22/21 06:13 PM (2 months, 25 days ago) |
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Yes! I've tried the cornmeal germination recipe to great success; I can't stop recommending it to people. I haven't conducted any formal experiments but just anecdotally germination seemed to occur far more quickly. I made 3 germination plates with subtropicalis using a modification to the recipe in this book and 3 with my usual BRF/yeast recipe. The three cornmeal dishes all showed signs of germination within just 2 days while the quickest of the 3 BRF plates germinated in 4 days and the slowest in 8! The same speed was noticed for some APE and a 1 year old mexicana print. Also anecdotally, they (the cornmeal plates) seem a little more prone to contamination but that might just be a coincidence.
I used the following:
10 g cornmeal
1 g dry active yeast
1/4 teaspoon light karo syrup
6 g agar powder
to 500 ml of water.
I'd love for some others to try this out and share their experiences.
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Edited by multifractal (07/22/21 08:14 PM)
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sandman420
Saint PP
Registered: 06/17/04
Posts: 3,379
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Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27398316 - 07/22/21 07:50 PM (2 months, 25 days ago) |
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The faster cornmeal shows more contams? That makes sense if it works better for germinating.
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Have no fear of perfection, you'll never reach it.
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multifractal
Student
Registered: 05/09/15
Posts: 816
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Re: Interesting agar recipes from the Radical Mycology book [Re: sandman420]
#27398343 - 07/22/21 08:18 PM (2 months, 25 days ago) |
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Yeah, the ones that showed contamination had a far greater variety of contaminant organisms than I usually see when a plate goes bad. The contaminations also showed themselves within the first 12 hours and i saw no additional ones after that.
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Edited by multifractal (07/22/21 08:19 PM)
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