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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Help me find my error in WBS prep? [Re: Sockadin]
    #26788859 - 06/28/20 04:07 AM (3 years, 7 months ago)

Quote:

Sockadin said:
The reason I said alot of people probably read this but didn't respond is because in my opinion early in the thread there was some iffy advise given to you. Dare I say bad.




Hmm isnt it better to correct that instead of just saying it like that? If I have given advice that is so bad I would love to know so that I may not say the same thing again in ignorance. Likewise if its someone else. But going through the initial posts, what I see, OP was 100% sure it was his grain prep. He acknowledged that he had tried many different teks for grains all of them failed. So I pointed out that syringe is more likely to be the culprit. Obviously OP seems to follow teks very well, and sure it could be the jars themselves or the PC etc, which was also achnoledged by repliers. But usually when troubleshooting you start from the beginning and work your way forward. Or you can start from the end and work your way backward in the process. Either way you end up with the syringe as a question mark. And thus I gave OP the same suggestions trusted cultivators in these forums have given me: AGAR.

Edit: I hope I dont come across as an asshole or anything, Sockadin. You have previously given me awesome advice that helped me. And also OP is obviously struggling like many of us here, and I would not wish to make that struggle harder. My point is that I would love to be corrected if I have given bad advice, similarly if someone else gave bad advice its good for all of us to know so we may avoid it :smile:


Edited by redhandmat (06/28/20 04:45 AM)


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
Trusted Cultivator
Re: Help me find my error in WBS prep? [Re: redhandmat]
    #26789055 - 06/28/20 07:18 AM (3 years, 7 months ago)

Not you bro i just disagree with changing the cap and needle over and over again.

I reread it and might have misunderstood what he was saying. I think now he was saying to just using the syringe cap and skipping the needle when dropping water on the germination plate.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Help me find my error in WBS prep? [Re: Sockadin]
    #26789827 - 06/28/20 01:22 PM (3 years, 7 months ago)

Quote:

Sockadin said:
Not you bro i just disagree with changing the cap and needle over and over again.

I reread it and might have misunderstood what he was saying. I think now he was saying to just using the syringe cap and skipping the needle when dropping water on the germination plate.




Ah alright! yeah Im a noob and still learning a lot so I wasnt sure. Anyways, I hope OP gets some nice results with agar soon :smile:


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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,537
Loc: Taiwan
Re: Help me find my error in WBS prep? [Re: Sockadin]
    #26790373 - 06/28/20 05:00 PM (3 years, 7 months ago)

Quote:

Sockadin said:
I reread it and might have misunderstood what he was saying. I think now he was saying to just using the syringe cap and skipping the needle when dropping water on the germination plate.



:thumbup: exactly. you don't need a needle to squirt a little drop on a plate. it's just another contamination vector if you store it with the needle still attached (which most people do because they don't have extra needles).


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OfflineEntheoGod
Entheo
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Registered: 03/06/15
Posts: 648
Last seen: 8 months, 15 days
Re: Help me find my error in WBS prep? [Re: FriedEgg]
    #26791059 - 06/28/20 10:38 PM (3 years, 7 months ago)

Hey guys still working hard at it gonna be updating everything soon with pics and the progress. Just to be brief I have nothing growing in the agar I had made after tossing the first batch. No contams or mycelium. And a few of the jars are showing something but I’m fairly sure those are too dry.

The latest batch of seeds is too early to say anything but I followed the advice of fried egg when it comes to keeping the jars inside the pressure cooker til morning then inoculating them. Again that is the last spore syringe to grain I’ll be doing but had to not waste what I already had going!

I’ll be making new agar as soon as the new plates come in the mail and I’ll be dropping a seed that’s already colonized, onto a fresh agar plate to clean that bacterial myc up I’ll likely try using a pin or some mushie tissue from the only monotub I have going at the moment. And also gonna use the last bits of some syringes I have but I’ll put those on agar as well to try getting something clean and if nothing else, at least I learned something :smile: any more advice would be great as well as if you think 4 or 5 days is a long time seeing nothing on agar. No contams ,just nothing and I can see the spores that were placed on it. The agar looks a bit sweaty tbh. I’ll post pics soon as I get some more progress made towards clean spawn!


Edited by EntheoGod (06/28/20 10:40 PM)


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OfflineEntheoGod
Entheo
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Registered: 03/06/15
Posts: 648
Last seen: 8 months, 15 days
Re: Help me find my error in WBS prep? [Re: EntheoGod]
    #26794709 - 06/30/20 02:33 PM (3 years, 7 months ago)

I have a quick question and thought it best to share it on this post as opposed to making an entirely separate one.

Ive been seeing different agar pressure cooking times being thrown around and would like to get a sort of consensus or at least someone to tell me if I did it too long. I pressure cook the PastyPlates at 15psi for 45 minutes. I am noticing literally no growth in the latest agar plates I made. Not even a contamination. You can clearly see the spores yet nothing seems to have changed since day 1. Its been around about 5 or 6 days since inoculating them with as little spore solution as possible.

If I am being impatient I have no problem waiting at all. Just wanted to check and see if I should scrap those and make fresh agar so I could pressure cook for a shorter time. Not thinking that would be the problem but I thought it best to ask and get some opinions.

From my perspective, I think I am doing it properly and nothing should be wrong (other than potentially bad or dead spores) I have no idea why every spore in a syringe could be dead so I dont think that would really be a problem either.

For all I know, I have done everything right and just need to wait for results. Regardless, I will be making 8 more pasty plates today and was hoping I could still get a consensus on how long to pressure cook them. I see on his tek it says 40-45 minutes and then I see other TC's saying 20 minutes is all that is needed because any more could cook the sugars inside the agar. :eek: All these different times and different information is a bit confusing and makes me question whether I am doing it right to begin with..

I feel that I have to reiterate that I am not really being impatient so much as I am unaware of what to expect around what time frames really. I am willing to wait as long as needed. I keep reading a variety of different responses to older posts that say several different things. Some say I should wait 20 days some say if you dont see growth on agar within a week to scrap it. 

(gonna be using colonized grains or pins to inoculate the agar I plan on making soon.)

Thanks a ton for your help!


Edited by EntheoGod (06/30/20 02:38 PM)


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Offlinecheckmatsis
morphicvibrantdarkness
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Registered: 07/04/19
Posts: 105
Last seen: 2 months, 28 days
Re: Help me find my error in WBS prep? [Re: EntheoGod]
    #26795034 - 06/30/20 05:08 PM (3 years, 7 months ago)

Not sure if you've already seen this post, but:

https://www.shroomery.org/forums/showflat.php/Number/17052521


"Spores can take up to 3 weeks to germinate...even on agar." - PussyFart


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OfflineEntheoGod
Entheo
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Registered: 03/06/15
Posts: 648
Last seen: 8 months, 15 days
Re: Help me find my error in WBS prep? [Re: checkmatsis]
    #26795270 - 06/30/20 06:58 PM (3 years, 7 months ago)

Yea, I absolutely said I understand that it can take a while for anything to germinate. Where I am new to agar I trust a majority of what I read on here when its posted by TC's but the varying degrees of information can be pretty tedious to sift through. I find it difficult to understand whether its a bad thing if it takes more than a week to germinate or whether that's common for agar or spores in general when I come across multiple different answers to the same question. I do understand that everyone has different levels of success so the experience is subjective to a degree. But what would be considered the typical germination times for spore solutions put to agar? Like if you were to ask me when you should see growth on something like a spore syringe put on grains, I would, subjectively, say that if you don't see growth of some sort within 4 days then something is up. But again that's completely subjective and I know that its a fact that spores can take much longer to germinate. They CAN but is it a good sign if they do?

I hope I don't sound too high to have replied yet 🤣


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Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 8 hours, 58 minutes
Re: Help me find my error in WBS prep? [Re: EntheoGod]
    #26795331 - 06/30/20 07:21 PM (3 years, 7 months ago)

im sorry i didnt read all this whats your problem


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineEntheoGod
Entheo
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Registered: 03/06/15
Posts: 648
Last seen: 8 months, 15 days
Re: Help me find my error in WBS prep? [Re: tripdawg420]
    #26795394 - 06/30/20 07:38 PM (3 years, 7 months ago)

Ima make a different thread in a few days purely about my agar but for now I’d say this thread is pretty done. :smile: thanks for everyone’s input and help I look forward to the help in the future 😂.


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