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Jbrady5555
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What is happening in this jar? 1
#26793288 - 06/29/20 09:15 PM (3 years, 6 months ago) |
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What is the really thick thing going on in this jar? I’ve seen a few spots like this in some of my jars. Are they being left too long in the jar? Thanks for any info.
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Jbrady5555
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Re: What is happening in this jar? [Re: Jbrady5555]
#26793818 - 06/30/20 06:27 AM (3 years, 6 months ago) |
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Bump
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26793901 - 06/30/20 07:26 AM (3 years, 6 months ago) |
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verticillium infection, dry bubbles are forming
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AtmozFear
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Re: What is happening in this jar? [Re: sandman420]
#26793978 - 06/30/20 08:07 AM (3 years, 6 months ago) |
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really? I thought it was a pin.
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sandman420
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Re: What is happening in this jar? [Re: AtmozFear]
#26793985 - 06/30/20 08:10 AM (3 years, 6 months ago) |
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That's pretty much what the dry bubbles are. You can really see the funky matted look of the mycelium and how the "pin" has no head.
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AtmozFear
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Re: What is happening in this jar? [Re: sandman420]
#26793990 - 06/30/20 08:11 AM (3 years, 6 months ago) |
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oh ok
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Pastywhyte
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Re: What is happening in this jar? [Re: AtmozFear] 1
#26793996 - 06/30/20 08:17 AM (3 years, 6 months ago) |
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That isn’t Lecanicillium fungicola (which is the actual name for the mold that causes “dry bubble,” Verticillium no longer applies) which is very rare invitro. I would suspect it’s a cube pin which likely formed as a response to contam. Cubes will rarely pin in grain if the media is axenic. It’s such a poor pinning surface that the colony needs to be desperate to form pins on grain invitro.
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794001 - 06/30/20 08:26 AM (3 years, 6 months ago) |
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It absolutely is. This is what it would look like outside of the jar.
you can see the mycelium even has the same matted look. Vert is only rare invitro if it is naturally occurring. It is 10000% common invitro because it is a mycoparasite so if you start a culture that has it it in the og print it will be in the entire life cycle of the culture. And vert is not an incorrect name. It was only just started to be classified the new name after learning it fits a different type but it is still commonly called vert.
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Re: What is happening in this jar? [Re: sandman420]
#26794013 - 06/30/20 08:33 AM (3 years, 6 months ago) |
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Quote:
sandman420 said: It absolutely is. This is what it would look like outside of the jar.
you can see the mycelium even has the same matted look. Vert is only rare invitro if it is naturally occuring. It is 10000% common invitro because it is a mycoparasite so if you start a culture that has it it in the og print it will be in the entire life cycle of the culture.
You might want to learn the actual name of the mold you keep referring to. Verticillium is a genus of mold that effects plants.
While you’re not completely wrong that Lecanicillium can infect prints, it rarely is an issue. It doesn’t seem to like to germinate without the presence of mushroom mycelium and if the mushrooms spores germ first it’s easy to get them ahead. Lecanicillium doesn’t like lower temps so keep your plates under 75 and the mushrooms will outgrow it fast.
If you shoot spores into grain jars you’re a hack stuck in 2007 and should expect to have mold.
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794019 - 06/30/20 08:41 AM (3 years, 6 months ago) |
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If you take a clone from a fruit that has vert you will have vert invitro.
If you make a syringe or a plate from a print of a cap that had vert you will have vert invitro.
Saying vert is rare invitro is nonsense. It's a mycoparasite.
It is only considered to occur after casing in commercial farming because they know what to look for in the obvious signs before they spawn it while home hobbyists do not.
verticillium fungicola was the name of the organism and that is what everyone calls it and thats what the op has. Commonly just called vert in the mushroom community thank you very much.
Edited by sandman420 (06/30/20 08:48 AM)
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Re: What is happening in this jar? [Re: sandman420] 1
#26794028 - 06/30/20 08:47 AM (3 years, 6 months ago) |
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I suspect the OP has bacterial grain that pinned invitro. No where does he state what he used for inoculate or where the original material came from. For you to assume that he has Lecanicillium invitro is a huge assumption. I have seen more than my share of Lecanicillium so I’m pretty familiar with it. The OP’s jar looks bacterial with an invitro pin forming.
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794033 - 06/30/20 08:49 AM (3 years, 6 months ago) |
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It's not such a huge assumption when the literal dry bubble is staring you right in the face. That is no normal pin there is no head. That thing at the tip is just a piece of uncolonized grain.
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Pastywhyte
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Re: What is happening in this jar? [Re: sandman420]
#26794038 - 06/30/20 08:53 AM (3 years, 6 months ago) |
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You’ve never seen a pin without a cap? Shit man you got a lot of growing to do. Either way I don’t think anyone is capable of definitely saying what that is or isn’t. A single pic with no background info on the inoculate used, temps, etc isn’t going to be super definitive.
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794041 - 06/30/20 08:58 AM (3 years, 6 months ago) |
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IME Lecanicillium “dry bubble” looks dry and are more yellow/tan in cubes.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794058 - 06/30/20 09:08 AM (3 years, 6 months ago) |
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Thanks for all the responses. I inoculated with agar wedges from a 4th transfer plate.
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26794065 - 06/30/20 09:12 AM (3 years, 6 months ago) |
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Do you have any pics of the plate? Was it a clone? If so do you suspect that grow had a contam? Any pics from it?
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794076 - 06/30/20 09:20 AM (3 years, 6 months ago) |
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i think you know that if it was bacterial there would be large parts of un-colonized grain in the jar. yes I have seen pins without heads when very small primordia stage. But that is much too large to have 0 head. Much like me.
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Re: What is happening in this jar? [Re: sandman420]
#26794089 - 06/30/20 09:28 AM (3 years, 6 months ago) |
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Lol just because the bacteria isn’t pooling in the jar doesn’t mean it’s not there. In fact nearly every grain jar should be assumed to at least have the possibility of surviving bacteria.
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794101 - 06/30/20 09:35 AM (3 years, 6 months ago) |
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I think maybe we can at least agree that that there jar aint right
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Pastywhyte
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Re: What is happening in this jar? [Re: sandman420]
#26794107 - 06/30/20 09:39 AM (3 years, 6 months ago) |
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Yes it’s definitely not right. However if I’m correct he could leave the grain in the jar, case and top fruit if he wanted a print or some produce with minimal risk. If you’re right the whole thing should be trashed and every culture and print he has from that line needs to be triaged. He will need to either clean the fuck out of his whole house or quit growing for six months (six months is the average lifespan of Lecanicillium fungicola spores).
That all said, I strongly agree that he not spawn or expand that jar.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794419 - 06/30/20 12:17 PM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Yes it’s definitely not right. However if I’m correct he could leave the grain in the jar, case and top fruit if he wanted a print or some produce with minimal risk. If you’re right the whole thing should be trashed and every culture and print he has from that line needs to be triaged. He will need to either clean the fuck out of his whole house or quit growing for six months (six months is the average lifespan of Lecanicillium fungicola spores).
That all said, I strongly agree that he not spawn or expand that jar.
Wow we need to talk about this lol. I’m on vacation so I haven’t been very responsive sorry. I would like to speak with you guys later. I’ve got new jars now that are also getting that thick mat look.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26794489 - 06/30/20 12:49 PM (3 years, 6 months ago) |
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The short of it is AHEM Lecanicillium aka Verticillium Dry Bubble is a parasite type "infection" that spreads really sticky spores and can become a reocurring problem and infects the prints of crops and any clones taken from it
Quote:
Unfortunately, this disease can rapidly escalate into an epidemic and then it takes weeks to gradually reduce the spore load on the farm to get the disease back under control. This reduction in the spore load around a farm and in the houses is a very important component of control. How to reduce this spore load is the problem. First, the vector(s) that is spreading the spores must be identified and stopped. From the time of first symptom development the grower must look back to who or what was in contact with the crop about 10-14 days earlier; with warmer room temperatures or higher humidity, that time is shortened to about 7-10 days. Most often, dry bubbles spotted before the end of first break indicate that the crop was infested by something other than harvesting personnel or their equipment.
So you don't want me to be right about it. It's gross. And at this stage the vector would be simply growing with previously infected spores/clones, in addition to the damage you are doing to your growroom by spreading even more spores by continuing to grow it. So youd likely end up having to trace that line of clone back and destroy any prints that were made from it and etc. It would be an issue. You can see how it can get out of hand.
Edited by sandman420 (06/30/20 12:54 PM)
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Pastywhyte
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Re: What is happening in this jar? [Re: sandman420]
#26794524 - 06/30/20 01:08 PM (3 years, 6 months ago) |
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Lol okay man, you can copy paste as many 20 year old posts as you like.
I’m not saying OP doesn’t have a Lecanicillium issue, I’m saying it’s not visible in the picture. But the grain is most obviously bacterial, the pressed uncolonized grains are the major indicator.
Even if that was a “dry bubble” in the jar, it would mean the colony is pinning. Pinning invitro in cereal grain IME is most often seen as a response to bacteria.
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794532 - 06/30/20 01:12 PM (3 years, 6 months ago) |
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I was talking to the OP not you, just saying what it would be like if he DID have the infection. Relax friend. No bad joojoo. I was literally in agreement with your previous post about how if I was right he had a big problem.
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Re: What is happening in this jar? [Re: sandman420]
#26794558 - 06/30/20 01:24 PM (3 years, 6 months ago) |
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It’s a huge problem, white molds are the worst. I struggled with it for years. Worse than trich. Fortunately it’s not actually super common and as bodhi recently demonstrated, most people who worry they have it usually are looking at pseudomonas instead.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26794567 - 06/30/20 01:28 PM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: It’s a huge problem, white molds are the worst. I struggled with it for years. Worse than trich. Fortunately it’s not actually super common and as bodhi recently demonstrated, most people who worry they have it usually are looking at pseudomonas instead.
Hey if it can help y’all figure out just how bad my problem is, this is a pic from one of three shoeboxes all from the same jars as the one we have been talking about. I know there isn’t many mushies growing but a lot of side pins as well. Probably didn’t take care of my surface good since I was traveling.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26794590 - 06/30/20 01:38 PM (3 years, 6 months ago) |
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That's pretty sad looking foooor sure.
Could just be some bacteria. Heres some signs of vert to look for though as the crop ages:
Exploded stems bubbles aka blobs slimy brown or grey patch in center of cap downturned growth
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26794606 - 06/30/20 01:44 PM (3 years, 6 months ago) |
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The growth pattern is weak and looks bacterial for sure. But the pins do not look infected with white molds; Lecanicillium infection causes the fruits to slump, the base bowes out, the stipe starts to shatter or peel, and the caps turn greyish. Some pins will fail completely and turn into a cap less dry looking ball or bubble. Those pins look like they are battling something though, probably trich that sets in from the bacterial spawn. I like to call pins like those “trich pins.”
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26795032 - 06/30/20 05:08 PM (3 years, 6 months ago) |
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So I’m not so bummed that my first shoeboxes don’t seem to be turning out. I’m bummed because I see no difference in the other 12 jars I have going now. You guys seem to think these few pins of mine are battling something. If a full fruit produces, is it safe to consume? That’s the kind of thing I guess I really need to be learning now. Safety first.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26795063 - 06/30/20 05:24 PM (3 years, 6 months ago) |
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Yes it would be safe for nearly anyone to consume even in my worst case scenario. Some people can be sensitive to bacteria and molds so it's better if you dehydrate it under 150+f heat to be on the safest side and to go easier on your stomach.
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Jbrady5555
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Re: What is happening in this jar? [Re: sandman420]
#26795484 - 06/30/20 08:14 PM (3 years, 6 months ago) |
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Quote:
sandman420 said: Yes it would be safe for nearly anyone to consume even in my worst case scenario. Some people can be sensitive to bacteria and molds so it's better if you dehydrate it under 150+f heat to be on the safest side and to go easier on your stomach.
So here are my next 4 jars. They are colonizing twice as fast as the jars and boxes we have been discussing. Everything I have has come from one MS syringe but I’m like 6 transfers deep and all my agar seems really clean. If I do 6 transfers I may lose one to two. I even had some agar plates pin and I transferred the pin. Those look really good on agar.
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26795707 - 06/30/20 09:50 PM (3 years, 6 months ago) |
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Those jars look like the reason I regretted ever using oats. My apologies to those who followed my poor example.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26795916 - 06/30/20 11:20 PM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Those jars look like the reason I regretted ever using oats. My apologies to those who followed my poor example.
Please elaborate. And what do you use now?
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26796020 - 07/01/20 12:35 AM (3 years, 6 months ago) |
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I like rye and wheat. They are an easy prep and low CFU populations IME. WBS is a less forgiving prep and more expensive but, it’s easily sourced and has great inoculation power.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26796319 - 07/01/20 06:11 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: I like rye and wheat. They are an easy prep and low CFU populations IME. WBS is a less forgiving prep and more expensive but, it’s easily sourced and has great inoculation power.
So should I even make shoeboxes with those jars? I’ll look into rye or wheat next.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26796346 - 07/01/20 06:40 AM (3 years, 6 months ago) |
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Hell no dont make boxes with those. You just going to get a few stragglers that aren't even worth the 25 cents to dehydrate them.
I just wanted to put a good word out there for millet too. It is by far the cleanest grain that is available at my feed store, and super tiny size makes it the superior spawn. It can be hard to work with in jars though, hard to shake it gets way more dense than any other grain when colonized. Gotta add gypsum for jars for sure. A lot better for bags.
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Jbrady5555
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Re: What is happening in this jar? [Re: sandman420]
#26796354 - 07/01/20 06:50 AM (3 years, 6 months ago) |
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Quote:
sandman420 said: Hell no dont make boxes with those. You just going to get a few stragglers that aren't even worth the 25 cents to dehydrate them.
I just wanted to put a good word out there for millet too. It is by far the cleanest grain that is available at my feed store, and super tiny size makes it the superior spawn. It can be hard to work with in jars though, hard to shake it gets way more dense than any other grain when colonized. Gotta add gypsum for jars for sure. A lot better for bags.
Well crap. So if all my jars are coming out this way from a few different agar plates is it safe to say that I shouldn’t use any of my other ready to go agar plates. I’ve got 5 plates in the fridge grown out to about 75 percent of the surface area. Some of them were grown from agar pins.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26796359 - 07/01/20 06:54 AM (3 years, 6 months ago) |
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well not necessarily. Depends on the vector and the organsism and seems to be at least debatable. The plates could be clean and it could be grain prep or something.
But my opinion is that you have the parasite this just doesn't look like bacteria to me. Usually bacterial jars make a lot of heat and the get really wet looking from condensation, and there is typically some definite lines of demarcation where the mycelium makes a defining edge against the bacteria that I am not seeing. A pretty reliable test for bacteria is to give the jar a whiff ans see if it stinks. That's my opinion here not really interested in arguing about it or anything anymore I've said my piece.
Edited by sandman420 (07/01/20 07:01 AM)
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Jbrady5555
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Re: What is happening in this jar? [Re: sandman420]
#26796371 - 07/01/20 07:01 AM (3 years, 6 months ago) |
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Quote:
sandman420 said: well not necessarily. Depends on the vector and the organsism and seems to be at least debatable. The plates could be clean and it could be grain prep or something.
But my opinion is that you have the parasite this just doesn't look like bacteria to me. Usually bacterial jars make a lot of heat and the get really wet looking from condensation and there is some definite lines of demarcation where the mycelium makes a defining edge against the bacteria that I am not seeing.
Okay well I sure do appreciate the help. Later today in this thread I think I’ll sacrifice one of my agar plates and put some pics in this thread to let you guys have a look. I’ll show the oat brand I’m using and maybe y’all a bit about my grain prep. I’ve made two batches af jars. Both with a 2hr pressure cook. One was bods oat prep tek. The second was a easier prep without boiling. Just an overnight soak. But always 2 hrs at 15psi.
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Jbrady5555
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Re: What is happening in this jar? [Re: Jbrady5555]
#26796374 - 07/01/20 07:02 AM (3 years, 6 months ago) |
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I smell everything and it all smells like mushrooms. If I don’t use these jars what about casing the top of the jars just to see what happens? I’m all about trying different things to learn right now.
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sandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
#26796393 - 07/01/20 07:11 AM (3 years, 6 months ago) |
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Casing the jars to see what happens sounds like a fine plan. If they were bacterial and you spawned them the spawn usually just doesn't recover and ever grow so that would be bad. At least this way it's still consolidated and COULD fruit.
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Zakkery



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Re: What is happening in this jar? [Re: Jbrady5555]
#26796398 - 07/01/20 07:14 AM (3 years, 6 months ago) |
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Just stopping by to admire the constructive discourse in this thread. Good show boys.
Good luck to you OP, I'll be observing and learning.
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Pastywhyte
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Re: What is happening in this jar? [Re: Zakkery]
#26796483 - 07/01/20 08:12 AM (3 years, 6 months ago) |
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You can case and top fruit bacterial spawn. It’s not the best yield but it can still give you something if you have nothing. Here is an example of bacterial spawn that I just cases and top fruited directly. By not breaking up and exposing the colony to open air you limit how much damage mold is able to do.

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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26796814 - 07/01/20 11:27 AM (3 years, 6 months ago) |
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Awesome! Thank you all again. How thick of a casing layer do I want to use? 1/2”? All I have is coir. Leave open or some kind of lid? Darkness or light?
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Jbrady5555
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Re: What is happening in this jar? [Re: Jbrady5555]
#26798726 - 07/02/20 07:34 AM (3 years, 6 months ago) |
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Bumping just to get so guidance on how to case and fruit directly from my quart jars
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26798731 - 07/02/20 07:38 AM (3 years, 6 months ago) |
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Easiest way for most people is to remove lid, put a half inch of coir/verm on the top of the grains, then put a ziploc baggy over the jar mouth. Poke a hole or two in the bag for extra air flow if needed and mist if it gets dry. Should see a few fruits come up.
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SlyAgaric
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Re: What is happening in this jar? [Re: Pastywhyte]
#26798931 - 07/02/20 09:46 AM (3 years, 6 months ago) |
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Looks like a friendly shroom trying to grow to me
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26799588 - 07/02/20 02:49 PM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Easiest way for most people is to remove lid, put a half inch of coir/verm on the top of the grains, then put a ziploc baggy over the jar mouth. Poke a hole or two in the bag for extra air flow if needed and mist if it gets dry. Should see a few fruits come up.
Cool thanks
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Jbrady5555
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808579 - 07/07/20 09:13 AM (3 years, 6 months ago) |
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So here is my next few jars, they don’t seem to have that matted cinnamon roll icing look. Do they seem to be right or do you folks still think I have a problem?
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808582 - 07/07/20 09:17 AM (3 years, 6 months ago) |
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Quote:
Jbrady5555 said:

So here is my next few jars, they don’t seem to have that matted cinnamon roll icing look. Do they seem to be right or do you folks still think I have a problem?
The other jars were bacterial, these look like straight mold to me.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26808682 - 07/07/20 09:51 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said:
Quote:
Jbrady5555 said:

So here is my next few jars, they don’t seem to have that matted cinnamon roll icing look. Do they seem to be right or do you folks still think I have a problem?
The other jars were bacterial, these look like straight mold to me.
Haha well that sucks!
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808712 - 07/07/20 10:13 AM (3 years, 6 months ago) |
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What are you using to inoculate? Those jars just looks sketch af to me.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26808723 - 07/07/20 10:19 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: What are you using to inoculate? Those jars just looks sketch af to me.
Made a LI using a agar puck that was from 4 transfers deep. Looked clean but I’m new, haven’t seen a whole lot.
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808727 - 07/07/20 10:20 AM (3 years, 6 months ago) |
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Got any pics of the agar?
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26808744 - 07/07/20 10:28 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Got any pics of the agar?

Unfortunately no. If I remember right that jar ended up way overgrown though. All my myc looks the same though, dense growth. This pic was from a few transfers ago when I posted about the contamination in this pic.
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808779 - 07/07/20 10:45 AM (3 years, 6 months ago) |
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Well I might try top fruiting those jars as well but I wouldn’t spawn em. I would try and change things up; better lids (metal two piece are clumsy and lead to bad handling), try a different grain (I never liked oats, most of my worst failures were with oats), and avoid liquid inoculates until you get everything else dialed in.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26808834 - 07/07/20 11:17 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Well I might try top fruiting those jars as well but I wouldn’t spawn em. I would try and change things up; better lids (metal two piece are clumsy and lead to bad handling), try a different grain (I never liked oats, most of my worst failures were with oats), and avoid liquid inoculates until you get everything else dialed in.
Eh I already spawned them right to a shoebox, posted the pic later. They did pass the smell test at least. I bought some Pennington’s wild bird seed for finch’s that’s had no sunflower seeds. I’ll try it next with some clean myc that I have in the fridge waiting. When I make that post I’ll be sure to include a agar pic. Now I need to look up a good WBS tek.
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Jbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
#26808840 - 07/07/20 11:20 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: and avoid liquid inoculates until you get everything else dialed in.
What would you suggest, just using agar wedges put directly into the grain jars? I did this with my first few jars and it was terribly slow. That said I did only use a wedge the size of an agar transfer wedge. Inexperience...
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Pastywhyte
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Re: What is happening in this jar? [Re: Jbrady5555]
#26808898 - 07/07/20 11:45 AM (3 years, 6 months ago) |
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I use this. But more wedges is always better than less. Also I like to use pint jars as masters and them G2G them to six quarts or so. They colonize way faster than quarts and are easier to wield in a SAB.
https://www.shroomery.org/forums/showflat.php/Number/22601023
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sandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
#26809017 - 07/07/20 12:47 PM (3 years, 6 months ago) |
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I saw a mushroom farm that was using something similar to d3monic's agar hole punches to inoculate bags. They just used the 1/4" punch to make a ton of circles on the plate and lifted a bunch of the little circles into the bag and shake. I have no idea where I saw it but it was a cool video, I think it was a Hispanic looking lady if I remember right. Just another way you could transfer a plate to grain.
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Pastywhyte
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Re: What is happening in this jar? [Re: sandman420]
#26809032 - 07/07/20 12:54 PM (3 years, 6 months ago) |
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Lots of ways to get the job done.
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Culliganman360
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Re: What is happening in this jar? [Re: Pastywhyte]
#26816340 - 07/11/20 12:46 AM (3 years, 6 months ago) |
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been running into some bad mold problems.. good info in this thread👍 Thanks playas
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The shroomy 1
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Both of you are awesome! OP must just be like...
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AMU Q&A thread.
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