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OfflineJbrady5555
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What is happening in this jar? * 1
    #26793288 - 06/29/20 11:15 PM (8 days, 56 minutes ago)





What is the really thick thing going on in this jar? I’ve seen a few spots like this in some of my jars. Are they being left too long in the jar? Thanks for any info.


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OfflineJbrady5555
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Re: What is happening in this jar? [Re: Jbrady5555]
    #26793818 - 06/30/20 08:27 AM (7 days, 15 hours ago)

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Invisiblesandman420
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Re: What is happening in this jar? [Re: Jbrady5555]
    #26793901 - 06/30/20 09:26 AM (7 days, 14 hours ago)

verticillium infection, dry bubbles are forming


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OfflineAtmozFear
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Re: What is happening in this jar? [Re: sandman420]
    #26793978 - 06/30/20 10:07 AM (7 days, 14 hours ago)

really?  I thought it was a pin.


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Invisiblesandman420
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Re: What is happening in this jar? [Re: AtmozFear]
    #26793985 - 06/30/20 10:10 AM (7 days, 14 hours ago)

That's pretty much what the dry bubbles are. You can really see the funky matted look of the mycelium and how the "pin" has no head.


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Re: What is happening in this jar? [Re: sandman420]
    #26793990 - 06/30/20 10:11 AM (7 days, 13 hours ago)

oh ok  :takingnotes:


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Re: What is happening in this jar? [Re: AtmozFear] * 1
    #26793996 - 06/30/20 10:17 AM (7 days, 13 hours ago)

That isn’t Lecanicillium fungicola (which is the actual name for the mold that causes “dry bubble,” Verticillium no longer applies) which is very rare invitro. I would suspect it’s a cube pin which likely formed as a response to contam. Cubes will rarely pin in grain if the media is axenic. It’s such a poor pinning surface that the colony needs to be desperate to form pins on grain invitro.


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Invisiblesandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794001 - 06/30/20 10:26 AM (7 days, 13 hours ago)

It absolutely is. This is what it would look like outside of the jar.

you can see the mycelium even has the same matted look. Vert is only rare invitro if it is naturally occurring. It is 10000% common invitro because it is a mycoparasite so if you start a culture that has it it in the og print it will be in the entire life cycle of the culture. And vert is not an incorrect name. It was only just started to be classified the new name after learning it fits a different type but it is still commonly called vert.


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Re: What is happening in this jar? [Re: sandman420]
    #26794013 - 06/30/20 10:33 AM (7 days, 13 hours ago)

Quote:

sandman420 said:
It absolutely is. This is what it would look like outside of the jar.

you can see the mycelium even has the same matted look. Vert is only rare invitro if it is naturally occuring. It is 10000% common invitro because it is a mycoparasite so if you start a culture that has it it in the og print it will be in the entire life cycle of the culture.




You might want to learn the actual name of the mold you keep referring to. Verticillium is a genus of mold that effects plants.

While you’re not completely wrong that Lecanicillium can infect prints, it rarely is an issue. It doesn’t seem to like to germinate without the presence of mushroom mycelium and if the mushrooms spores germ first it’s easy to get them ahead. Lecanicillium doesn’t like lower temps so keep your plates under 75 and the mushrooms will outgrow it fast.

If you shoot spores into grain jars you’re a hack stuck in 2007 and should expect to have mold.


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Invisiblesandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794019 - 06/30/20 10:41 AM (7 days, 13 hours ago)

If you take a clone from a fruit that has vert you will have vert invitro.

If you make a syringe or a plate from a print of a cap that had vert you will have vert invitro.

Saying vert is rare invitro is nonsense. It's a mycoparasite.

It is only considered to occur after casing in commercial farming because they know what to look for in the obvious signs before they spawn it while home hobbyists do not.

verticillium fungicola was the name of the organism and that is what everyone calls it and thats what the op has. Commonly just called vert in the mushroom community thank you very much.


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Edited by sandman420 (06/30/20 10:48 AM)


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Re: What is happening in this jar? [Re: sandman420] * 1
    #26794028 - 06/30/20 10:47 AM (7 days, 13 hours ago)

I suspect the OP has bacterial grain that pinned invitro. No where does he state what he used for inoculate or where the original material came from. For you to assume that he has Lecanicillium invitro is a huge assumption. I have seen more than my share of Lecanicillium so I’m pretty familiar with it. The OP’s jar looks bacterial with an invitro pin forming.


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Invisiblesandman420
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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794033 - 06/30/20 10:49 AM (7 days, 13 hours ago)

It's not such a huge assumption when the literal dry bubble is staring you right in the face. That is no normal pin there is no head. That thing at the tip is just a piece of uncolonized grain.


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Re: What is happening in this jar? [Re: sandman420]
    #26794038 - 06/30/20 10:53 AM (7 days, 13 hours ago)

You’ve never seen a pin without a cap? Shit man you got a lot of growing to do. Either way I don’t think anyone is capable of definitely saying what that is or isn’t. A single pic with no background info on the inoculate used, temps, etc isn’t going to be super definitive.


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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794041 - 06/30/20 10:58 AM (7 days, 13 hours ago)

IME Lecanicillium “dry bubble” looks dry and are more yellow/tan in cubes.



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OfflineJbrady5555
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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794058 - 06/30/20 11:08 AM (7 days, 13 hours ago)

Thanks for all the responses. I inoculated with agar wedges from a 4th transfer plate.


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Re: What is happening in this jar? [Re: Jbrady5555]
    #26794065 - 06/30/20 11:12 AM (7 days, 12 hours ago)

Do you have any pics of the plate? Was it a clone? If so do you suspect that grow had a contam? Any pics from it?


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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794076 - 06/30/20 11:20 AM (7 days, 12 hours ago)

i think you know that if it was bacterial there would be large parts of un-colonized grain in the jar. yes I have seen pins without heads when very small primordia stage. But that is much too large to have 0 head. Much like me.


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Re: What is happening in this jar? [Re: sandman420]
    #26794089 - 06/30/20 11:28 AM (7 days, 12 hours ago)

Lol just because the bacteria isn’t pooling in the jar doesn’t mean it’s not there. In fact nearly every grain jar should be assumed to at least have the possibility of surviving bacteria.


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Re: What is happening in this jar? [Re: Pastywhyte]
    #26794101 - 06/30/20 11:35 AM (7 days, 12 hours ago)

I think maybe we can at least agree that that there jar aint right


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Re: What is happening in this jar? [Re: sandman420]
    #26794107 - 06/30/20 11:39 AM (7 days, 12 hours ago)

Yes it’s definitely not right. However if I’m correct he could leave the grain in the jar, case and top fruit if he wanted a print or some produce with minimal risk. If you’re right the whole thing should be trashed and every culture and print he has from that line needs to be triaged. He will need to either clean the fuck out of his whole house or quit growing for six months (six months is the average lifespan of Lecanicillium fungicola spores).

That all said, I strongly agree that he not spawn or expand that jar.


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