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Jus
Stranger
Registered: 09/27/19
Posts: 44
Last seen: 8 months, 6 days
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Jus a first time grow log
#26791296 - 06/29/20 03:36 AM (3 years, 8 months ago) |
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We start our journey months ago, hunting for libs in the peaks of Derbyshire after a substantial drive. "Fuck this," he thinks to himself, "let's grow shrooms!"
Cue months of research and combing through threads, reading TEKs and plotting my grow.
I decided to start with a spore syringe on agar, transfer until clean, use a wedge to colonise grain, then go to shoeboxes and grain to grain transfers.
We start terribly with several botched agar recipes, lots of contamination, and no Golden Teacher spores germinating.
Some cool bacteria though. Wrong hobby, but happy little accidents and all that.
I read up that spore prints may be easier, and at this point I'm willing to accept I've either permanently contaminated my spore vial, I'm not getting any/enough spores in the solution on the end of my inoculation loop, or some other combination of lack of experience, skill and luck.
I order an Albino A+ print, and successfully germinate spores.
And after several days, some nasty spots on some plates:
A few days before that last picture it was to transfer. As is the running theme I fuck this up royally the first time:
As you can see, the agar was clumping when I poured. I'd made a smaller batch and let it cool down too long whilst prating about with the SAB.
I don't even know how it got so contaminated, maybe that curse I said upon it when I realised I'd fucked up has manifested.
The cunks I'd taken are starting to heal:
Either way, I try again and it goes very well:
(Apart from one or two dropped sections...)
Next up, I'll be making control grain jars and drop in a blank agar wedge, wait and see if I make a jar of contamination. That'll give me time to keep transferring until I get a safe plate, which I can then keep going for some mono cultures whilst making some random genetics shoeboxes.
Stay tuned.
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Rathilien
Weed Reclamation Unit
Registered: 06/17/20
Posts: 108
Loc: Melbourne
Last seen: 11 months, 11 days
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Re: Jus a first time grow log [Re: Jus]
#26791309 - 06/29/20 03:47 AM (3 years, 8 months ago) |
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Quote:
Jus said: I don't even know how it got so contaminated, maybe that curse I said upon it when I realised I'd fucked up has manifested.
LOL As a new (about to begin) grower myself, I have a feeling this is going to resonate with me real soon.
Keep fighting the good fight!
-------------------- **Aussies PM Me if You're Having Trouble Finding Spores!** Follow my progress at my journal Edible Weeds I wouldnโt say Iโm a tree-hugging hippy, but I do like to give those thick-ass trees a solid slap on their trunks as I walk by.
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jcm4620
Stranger
Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 month
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Re: Jus a first time grow log [Re: Rathilien]
#26791334 - 06/29/20 04:10 AM (3 years, 8 months ago) |
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syringes suck usually for plates they can be very messy. i bet u will have better results with a print. are u in the states?? if so i can send u one. but also that is some clear ass fucking agar man๐๐ also good job on doing the research before just diving in and doing it as you go. and good move on using agar right from the get go. i have no doubt your first grow will be a good one. ๐๐ welcome to shroomery man
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Jus
Stranger
Registered: 09/27/19
Posts: 44
Last seen: 8 months, 6 days
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Re: Jus a first time grow log [Re: jcm4620]
#26791345 - 06/29/20 04:26 AM (3 years, 8 months ago) |
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I'm UK based, but thanks for the offer! I'm counting down the posts until I can do some trading with the prints from this grow.
My agar technique has come a long way from the first few pics.
I started with BRF agar and food colouring that was heat sensitive, so I had butt loads of sediment Now I use LME agar which I filter before pressure cooking. Poured correctly it produces a nice clear agar
It looks great under the microscope.
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jcm4620
Stranger
Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 month
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Re: Jus a first time grow log [Re: Jus]
#26791396 - 06/29/20 05:45 AM (3 years, 8 months ago) |
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nice. id send u a print but it seems like everytime i have tried to send a print to someone in the eu they have never gotten them. i think they are being taken in customs or somethin cuz i have tried several times to send prints to the uk germany and france and every single one never has never made it to the destination. so now i just dont bother for that reason only not cuz i have somethin against ya or anything lol but the good news is there is ppl on here that are also in the eu that u can trade with
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Jus
Stranger
Registered: 09/27/19
Posts: 44
Last seen: 8 months, 6 days
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Re: Jus a first time grow log [Re: jcm4620]
#26794114 - 06/30/20 09:42 AM (3 years, 8 months ago) |
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No worries, friend, when I get some nice prints I'll try and send you some to see if it works the other way around.
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